Ayşegül ERDOĞAN, Zeliha DEMİREL, Meltem Conk DALAY, Ahmet E. EROĞLU

Fucoxanthin Content of Cylindrotheca Closterium and Its Oxidative Stress Mediated Enhancement

Fukoksantinin diatomlar tarafından üretimi düşük maliyet, kolaylık ve çeşitlilik nedeniyle alternatif bir araştırma alanı haline gelmiştir. Bu çalışmada, Cylindrotheca closterium'da bulunan fukoksantin miktarı ve kültür koşullarına uygulanan oksidatif stresin, bu içeriği nasıl arttırdığı araştırılmıştır. Bu amaçla,ekstraksiyon parametreleri optimize edilmiş ve en yüksek fukoksantin derişimi ( 6,58 mg g ) 40 oC''de ve 15,0 dakika içinde elde edilmiştir. Daha sonra, fukoksantin miktarı, NaOCl ve Fe2+ varlığında 10,15 mg g - 1 a kadar ulaşmıştır. Bu çalışma ile ilk kez bir diatomun kültür koşullarına uygulanan oksitafis stres ile fukoksantin miktarındaki değişim gözlenmiştir. Bu nedenle; söz konusu çalışma, sonuçları ve mekanizmalar hakkında yapılan değerlendirmeler ile fukoksantinin diğer diatomlarda da zenginleştirilmesi açısından bir referans olabilir.

Cylindrotheca closterium'un Fukoksantin İçeriği ve Oksidatif Stres ile Arttırılması

Production of fucoxanthin by diatoms has become an alternative research area due to their low cost, convenience and diversity. The fucoxanthin content of Cylindrotheca closterium and its enhancement by altering the cultivation conditions via oxidative stress were investigated in this study. For this purpose, the extraction parameters were optimized and the highest fucoxanthin concentration (6.58 mg g-1) was achieved within 15.0 minutes at 40 oC. Then, this yield reached to 10.15 mg g-1in the presence of NaOCl and Fe2+. It is worth noting that, this is the first time that the effect of oxidative stress on fucoxanthin production in diatom has been studied according to our knowledge. Therefore, the results of this study and the discussion about the mechanisms can be a reference for the enrichment of fucoxanthin from other diatoms.

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