Comparative study of α-amylase activity in three Cyprinid species of different feeding habits from Southern Iraq
Bu çalışma, Garma Nehrinde (Basrah, Güney Irak) yaşayan üç Sazangil türünün ait mide özütündeki α-amilaz aktivitesine ait karşılaştırmalı verileri göstermektedir. Bu türler, Bunni Barbus sharpeyi (otobur), pullu sazan Cyprinus carpio (etotobur) ve shilik Aspius vorax (etobur). Çalışma aynı zamanda Garma Nehrinden toplanan ile havuzda yetiştirilmiş pullu sazanların enzim aktivitesi arasındaki farklılığı da araştırmaktadır. Özgül α-amilaz aktivitesi, araştırılan üç türün beslenme alışkanlıklarından belirgin bir şekilde etkilenmiştir. α-amilaz aktivitesine ait değerler, otobur bunni’de ortalama protein 1,84 U $mg^ {-1}$ olmuştur. Etotobur pullu sazanınkiyle karşılaştırıldığında anlamlı bir üstünlük (P
Güney Irak’ta farklı beslenme alışkanlıkları olan üç Sazangil türünde α-amilaz aktivitesi üzerine karşılaştırmalı çalışma
This work provides a comparative data of α-amylase activity in the gut extract of three Cyprinid species inhabiting Garma River (Basrah Governorate, Southern Iraq). Those species are bunny Barbus sharpeyi (herbivorous), common carp Cyprinus carpio (omnivorous), and shilik Aspius vorax (carnivorous). The study also investigate the discrepancy between the activity of the enzyme in common carp collected from Garma river with those reared in ponds. The specific activity of α- amylase has apparently been influenced by feeding habits of the three studied species. Values of α-amylase activity averaged 1.84 U $mg^ {-1}$ protein in the herbivorous bunny. It showed a significant (P<0.01) superiority to that of the omnivorous common carp (1.33 U $mg^ {-1}$ protein) which, in turn, showed a significant (P<0.01) superiority to that of the carnivorous shilik (0.76 U $mg^ {-1}$ protein). Omnivorous common carp which was collected from fish ponds showed a significantly (P<0.01) higher value of α-amylase specific activity which reached 1.92 U $mg^ {-1}$ protein when compared with the value recorded in common carp collected from Garma River which reached 1.33 U $mg^ {-1}$ protein.
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