Junwei WANG, Hongzhan LIU, Gaisheng ZHANG, Junsheng WANG, Qingsong BA, Yulong SONG, Pengfei ZHANG, Shoucai MA, Na NIU, Long CHEN, Huixue CHE

THE RELATIONSHIP BETWEEN MALE STERILITY AND MEMBRANE LIPID PEROXIDATION AND ANTIOXIDANT ENZYMES IN WHEAT (Triticum aestivum L.)

Malondialdehyde (MDA), which is thought to be important inducible factor of cell apoptosis if excessively accumulated in cells, is often regarded as a widely used marker of oxidative lipid injury whose concentration varies in response to biotic and abiotic stress. To elucidate the metabolic mechanism of ROS production and scavenging of the male sterility, normal fertility and male sterility wheat, including genetic and physiologically male sterility, by comparing MDA content and antioxidant enzymes changes in the sterile and fertile leaves, spikes, florets, rachillaes, ovaries and anthers at different developmental anther stages, were employed for studying the relationship between the male sterility and membrane lipid peroxidation, antioxidant enzymes. The results showed that tissue of male sterility had roughly higher content of MDA than corresponding fertile plants, and ovary performance was not obvious but most obvious in anther. Simultaneously, the higher activities of antioxidant enzymes were measured in the anthers of male sterility plant, in which an increase of ROS in anthers of abortion stage had an inducible effect on the antioxidant enzymes. MDA content and scavenging-enzyme activities had difference in the sterile and fertile leaves, spikes, florets, rachillaes at different anther stages, indicating that different tissues of ROS metabolism had noticeable difference in male sterility and normal wheat. It can be considered that the disorder of scavenging-enzyme activities and accumulation of MDA in the male organ, and supplier of nutrition blades were causing a further substance metabolism disorder, energy loss, leading to the occurrence of male sterility.

PDF

___

Ba, Q.S., G.S. Zhang, J.S. Wang, H.X. Che, H.Z. Liu, N. Niu, S.C. Ma, J.W. Wang. 2013.Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.). Can J Plant Sci. 93:1-7.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci Res.14:93-107.
Banga, S., K. Labana, S.K. Banga. 1984. Male sterility in Indian mustard (Brassica juncea (L.) Coss.)--a biochemical characterization. Theor Appl Genet. 67:515-519.
Bhaskaran, J. and R. Panneerselvam. 2013. Accelerated Reactive Oxygen Scavenging System and Membrane Integrity of Two Panicum Species Varying in Salt Tolerance. Cell Biochem Biophys.3:885-892.
Darkó, É., H. Ambrus, É. Stefanovits-Bányai, J. Fodor, F. Bakos, B. Barnabás. 2004. Aluminium toxicity, Al tolerance and oxidative stress in an Al-sensitive wheat genotype and in Altolerant lines developed by in vitro microspore selection. Plant Sci. 166:583-591.
Deng, M. H., J.F. Wen, J.L. Huo, H.S. Zhu, X.Z. Dai, Z.Q. Zhang, H. Zhou, X.X. Zou. 2012. Relationship of metabolism of reactive oxygen species with cytoplasmic male sterility in pepper (_____ i> Capsicum annuum_____/i> L.). Scientia Hortic.134:232-236.
Dou, X.Y., K.Z. Yang, Y. Zhang, W. Wang, X.L. Liu, L.Q. Chen, X.Q. Zhang, D. Ye. 2011.WBC27, an Adenosine Tri- phosphate-binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis. J Integr Plant Biol. 53:74-88.
Draganic, I., S. Lekic, T. Brankovic, G. Todorovic. 2011. Fatty acids and tocopherol content in sunflower seeds affected by accelerated ageing and priming with antioxidant solutions. Turk J Field Crops. 16, 100-104.
Fridovich I. 1978. The biology of oxygen radicals. Science. 201:875-880.
Gill, S.S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch. 48:909-930 . Hodges, D.M., J.M. DeLong, C.F. Forney, R.K. Prange. 1999. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta. 207:604-611.
Jia Z., W.H. Lin, H.B. Luo, L.Z. Sun, C. Liu. 2009. Comparison the activities of peroxidase, catalase, superoxide dismutase and peroxidase zymogram in C-cytoplasmic male sterility line on maize. Journal of Maize Sciences 6:014.
Jiang, M. and J. Zhang. 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol. 42:1265-1273.
Jiang, P.D., X.Q. Zhang, Y.G. Zhu, W. Zhu, H.Y. Xie, X.D. Wang. 2007. Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration. Plant Cell Rep. 26:1627-1634.
Li, S.Q., C.X. Wan, J. Kong, Z.J. Zhang, S.Y. Li, Y.G. Zhu. 2004. Programmed cell death during microgenesis in a Honglian CMS line of rice is correlated with oxidative stress in mitochondria. Funct Plant Biol. 31:369-376.
Liu, Z.N., X.L. Yu, F.Z. Wang, S. Hu, Y.P. Liu, G. Lu. 2012. Physiological, biochemical, and molecular characterization of a new female sterile mutant in turnip. Plant Growth Regul. 68:239-248.
Li, M.H., X.Y. Zhang, Y.F. Fan, Y.K. Li, Y.L. CAO. 2008. A study on activated oxygen metabolism in the male sterile plants of lycium barbarum. Acta Agriculturae Universitatis Jiangxiensis 5:009.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405-410.
Ou, L.J., Z.Q. Zhang, X.Z. Dai, X.X. Zou. 2013. Photooxidation Tolerance Characters of a New Purple Pepper. PloS One. 8:e63593.
Polidoros, A.N. and J.G. Scandalios. 1999. Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiol Plantarum. 106:112-120.
Selote, D.S. and R. Khanna-Chopra 2004. Drought-induced spikelet sterility is associated with an inefficient antioxidant defence in rice panicles. Physiol Plantarum. 121:462-471.
Smith, A.M., K. Denyer, C.R. Martin. 1995. What controls the amount and structure of starch in storage organs? Plant Physiol. 107:673.
Sunkar, R., D. Bartels, H.H. Kirch. 2003. Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. Plant J. 35:452-464.
Tripathi, D., S. Mehta, N. Rao. 1983. Soluble proteins and isoenzymes from seeds of diverse male steriles of sorghum,(Sorghum bicolor (L.) Moench). Theor Appl Genet. 64:325-331.
Wan, C.X., S.Q. Li, L. Wen, J. Kong, K. Wang, Y.G. Zhu. 2007. Damage of oxidative stress on mitochondria during microspores development in Honglian CMS line of rice. Plant Cell Rep. 26:373-382.
Wang, J.S., G.S. Zhang, L. Yuan, M.Z. Zhang, N. Niu, S.C. Ma, J.X. Ye. 2009. Metabolism of reactive oxygen species of physiological male-sterile anther induced by chemical hybrid agent in wheat. Acta Botanica Boreali-Occidentalia Sinica 7:013.
Wang Z., Y.X. Zhang, Z.B. Huang, L. Huang. 2008. Antioxidative response of metal-accumulator and nonaccumulator plants under cadmium stress. Plant Soil. 310:137-149.
Warmke, H.E., S.L.J. Lee. 1978. Pollen abortion in T cytoplasmic male-sterile corn (Zea mays): a suggested mechanism. Science. 200:561-563.
Winterbourn, C.C., R. Hawkins, M. Brian, R. Carrell. 1975. The estimation of red cell superoxide dismutase activity. J Lab Clin Med. 85:337-341.
Fatma, A. T., Lker. Emre, T. Muzaffer. 2010. A study to incorporate high protein content from tetraploid wheat (T. turgidum dicoccoides) wheat (T. aestivum vulgare). Turk J Field Crops. 15(1): 69-72