Isolation of different genotypes in 'Başçıftlik Beyazı' potato landrace using SSR markers

Isolation of different genotypes in 'Başçıftlik Beyazı' potato landrace using SSR markers

Potato is an important crop in Turkey. Along with modern cultivars, there are some potato landrace cultivars grown mainly for their superior taste. ‘Başçiftlik Beyazı’ is such a landrace cultivar grown in the Mid-Black Sea region of Turkey and used as baked potato. Since this cultivar is not registered, certified seed is not available. Therefore, tuber production suffers from serious yield and quality losses mainly due to viral diseases. The aim of the present study was to determine a main genotype and different genotypes within the cultivar using 16 SSR markers. SSR analyses were conducted over 191 ‘Başçiftlik Beyazi’ plants. Nine of the 16 SSR markers were polymorphic. The data showed that landrace cultivar ‘Başçiftlik Beyazi’ consisted of 23 different genotypes. The same marker profile was present in 150 of the 191 plants. SSR analyses conducted on some modern and landrace cultivars grown in the region showed that the different genotypes were not the remnants of the previous years’ potato crop. This variation could be directly used in potato breeding. SSR marker data also indicated that three local cultivars with different names were actually the same.

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  • Feingold, S., J. Lloyd, N. Norero, M. Bonierbale, J. Lorenzen. 2005. Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.) Theoretical Applied Genetics 111:456-466.
  • Ghislain, M., D.M. Spooner, F. Rodriguez, F. Villamon, J. Nunez, C. Vasquez, R. Waugh, M. Bonierbale. 2004. Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical Applied Genetics 108:881-890.
  • Ispizua, V.N., I.R. Guma, S. Feingold, A.M. Clausen. 2007. Genetic diversity of potato landraces from Northwestern Argentina assessed with simple sequence repeats (SSRs). Genetics Resources and Crop Evolution 54:1833-1848.
  • Milbourne, D., R. Meyer, J.E. Bradshaw, E. Baird, N. Bonar, J. Provan, W. Powell, R. Waugh. 1997. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular Breeding 3:127-136.
  • Milbourne, D., R. Meyer, A.J. Collins, L.D. Ramsay, C. Gebhardt, R. Waugh. 1998. Isolation, characterisation and mapping of simple sequence repeat loci in potato. Molecular and General Genetics 259:233-245.
  • Moisan-Thiery, M., S. Marhadour, M.C. Kerlan, N. Dessenne, M. Perramant, T. Gokelaere, Y. Le Hingrat. 2005. Potato cultivar identification using simple sequence repeats markers (SSR). Potato Research 48:191-200.
  • Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583-590.
  • Rafalski, J.A., J.M. Vogel, M. Morgante, W. Powell, C. Andre, S.V. Tingey. 1996. Generating and using DNA markers in plants. In: Nonmammalian genomic analysis (B. Birren, E. Lai, Eds.), A practical guide. Academic Press, San Diego, pp 75-134.
  • Reid, A., E.M. Kerr. 2007. A rapid simple sequence repeat (SSR)-based identification method for potato cultivars. Plant Genetic Resources 5:7-13.
  • Ruiz de Galarreta, J.I., L. Barandalla, R. Lorenzo, J. Gonzalez, D.J. Rios, E. Ritter. 2007. Microsatellite variation in potato landraces from the island of La Palma. Spanish Journal of Agricultural Research 5:186-192.
  • Yeh, F.C., R.C. Yang, T.B.J. Boyle, Z.H.Ye, J.X. Mao. 1997. POPGENE the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada (program available at http://www.ualberta.ca/fyeh/).
  • Yildirim, A., N. Kandemir, Ö.Ateş Sönmezoğlu, T.L. Güleç. 2009. Transferability of microsatellite markers among cool season cereals. Biotechnology & Biotechnological Equipment” 23:3:1299-1302.
  • Zimmerer, K.S., D.S. Douches. 1991. Geographical approaches to crop conservation: the partitioning of genetic diversity in Andean potatoes. Economical Botany 45:2:176-189.