DRAGANIC Ivana, Slavoljub LEKIC, Tatjana BRANKOVIC, Goran TODOROVIC

Fatty acids and tocopherol content in sunflower seeds affected by accelerated ageing and priming with antioxidant solutions

Effects of accelerated ageing on the contents of both, fatty acids and tocopherol in sunflower seeds, and the influence of priming with antioxidant solutions on the tocopherol content were observed in the present study. Accelerated ageing did not affect contents of linoleic (C18:2), oleic (C18:1), stearic C18:0) and palmitic (C16:0) acids. It was determined that the contents of α-, β- and γ-tocopherol decreased in seeds subjected to accelerated ageing and in seeds primed prior to accelerated ageing. Priming with antioxidant solutions prior to accelerated ageing differently affected the contents of α-, β- and γ-tocopherol in seeds. Furthermore, seeds primed with a solution of a lower concentration of tocopherol and simultaneously primed with a solution of all three antioxidant substances (ascorbic acid, tocopherol and glutathione) had a higher content of α-tocopherol after accelerated ageing in relation to seeds which were not primed. Seeds primed with solutions of ascorbic acid, tocopherol (higher concentration) and glutathione had a lower content of α-tocopherol than seeds which were not primed prior to accelerated ageing. The total content of β- and γ-tocopherol in primed seeds were significantly lower (p ≥ 0.05) than in seeds which were not primed prior to accelerated ageing, except in seeds simultaneously primed with the solution of all three antioxidant substances.

PDF

___

Bailly, C., A. Benamar, F. Corbineau, D. Côme, 1998. Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiol. Plant. 104: 646-652.
Bailly, C., R. Bogatek-Leszczynska, D. Côme, F.Corbineau, 2002. Changes in activities of antioxidant enzymes and lipoxygenaze during growth of sunflower seedlings from seeds of different vigour. Seed Sci. Res. 12: 47-55.
Bartosz, G., 1997. Oxidative stress in plants. Acta Physiol. Plant. 19: 47-64.
Branković, T., 2005. Isolation of tocopherols from vegetable oils using optimized saponification procedure in aim to determine them by liquid chromatography. Master thesis. Belgrade (in serbian).
Corbineau, F., C. Gay-Mathieu, D. Vinel, D. Côme, 2002. Decrease in sunflower (Helianthus annuus) seed viability caused by high temperature as related to energy metabolism, membrane damage and lipid composition. Physiol. Plant. 116: 489–496.
Demurin, Y., 1993. Genetic variability of tocopherol composition in sunflower seeds. Helia. 16: 59-62.
Fielding, J.L., A. Goldsworthy, 1980. Tocopherol levels and ageing in wheat grains. Ann. Bot. 46: 453-456.
Fryer, M.J., 1992. The antioxidant effects of thylakoid vitamin E (α-tocopherol). Plant Cell Environ. 15: 381-392.
Fukuzawa, K., A. Tokumura, S. Ouchi, H. Tsukatani, 1982. Antioxidant activities of tocopherols on Fe2+-ascorbate-induced lipid peroxidation in lechitin liposomes. Lipids. 17: 511-513.
Gidrol, X., H. Serghini, A. Noubhani, B. Mocouot, P. Mazliak, 1989. Biochemical changes induced by accelerated aging in sunflower seeds. I. Lipid peroxidation and membrane damage. Physiol. Plant. 76: 591–597.
Halliwell, B.H., J.M.C. Gutteridge, 2007. Free radicals in biology and medicine. Oxford, UK: Oxford University Press.
Harrington, J.F., 1972. Seed storage and longetivity. In: Kozlowski, T.T (edr), Seed Biology. Vol. III. Academic Press, New York, London, pp. 145-245.
Leprince, O., G.A.F. Hendry, B.D. McKersie, 1993. The mechanisms of dessication tolerance in developing seeds. Seed Sci. Res. 3: 231-246.
Lin, S.S., R.S. Pearce, 1990. Changes in lipids of bean seeds (Phaseolus vulgaris) and corn caryopses (Zea mays) aged in contrasting environments. Ann. Bot. 65: 451-456.
Мerritt, D.J., T. Senaratna, D.H. Touchell, K.W. Dixon, K. Sivasithamparam, 2003. Seed ageing of four Western Australian species in relation to storage environment and seed antioxidant activity. Seed Sci. Res. 13: 155-165.
Noctor, G., C.H. Foyer, 1998. Ascorbate and glutathion: keeping active oxygen species under control. Ann. Rev. Plant Physiol. Plant Mol. Bio. 49: 249-279.
Ponquett, R.T., M.T. Smith, G. Ross 1992. Lipid autoxidation and seed ageing: putative relationships between seed longevity and lipid stability. Seed Sci. Res. 2: 51-54.
Priestley, D.A., A.C. Leopold, 1979. Absence of lipid oxidation during accelerated aging of soybean seeds. Plant Physiol. 63: 726-729.
Priestley, D.A., A.C. Leopold, 1983. Lipid changes during natural aging of soybean seeds. Physiol. Plant. 59: 467–470.
Priestley, D.A., M.B. McBride, A.C. Leopold, 1980. Tocopherol and organic free radical levels in soybean seeds during natural and accelerated aging. Plant Physiol. 66: 715-719.
Pukacka, S., P.J.C. Kuiper, 1988. Phospholipid composition and fatty acid peroxidation during ageing of Acer plantanoides seeds. Physiol. Plant. 72: 89-93.
Pukacka, S., 1991. Changes in membrane lipid components and antioxidant levels during natural ageing of seeds of Acer platanoides. Physiol. Plant. 82: 306–310.
Pukacka, S., E. Ratajczak, 2007. Age-related biochemical changes during storage of beech (Fagus sylvatica L.) seeds. Seed Sci. Res. 17: 45-53.
Ratajczak, E., S. Pukacka, 2005. Decrease in beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition. Acta Physiol. Plant. 27: 3-12.
Sattler, S.E., L.U. Gilliland, M. Magallanes-Lundback, M. Pollard, D. DellaPenna, 2004. Vitamin E is Essential for Seed Longevity and for Preventing Lipid Peroxidation during Germination. Plant Cell, 16: 1419-1432.
Seal, C.E., R. Zammit, P. Scott, T.J. Flowers, I. Kranner, 2010a. Glutathione half-cell reduction potential and α-tocopherol as viability markers during the prolonged storage of Suaeda maritima seeds. Seed Sci. Res. 20: 47-53.
Seal, C.E., R. Zammit, P. Scott, D.O. Nyamongo, M.I. Daws, I. Kranner, 2010b. Glutathione half-cell reduction potential as a seed viability marker of the potential oilseed crop Vernonia galamensis. Ind. Crop. Prod. 32: 687-691.
Senaratna, T., J.F. Gusse, B.D. McKersie, 1988. Age-induced changes in cellular membranes of imbibed soybean seed axes. Physiol. Plant. 73: 85–91.
Sharma, K.D., 1977 Biochemical changes in stored oil seeds. Indian J. Agric. Res. 11: 137-141.
Socrates, A., С.А. Kaloyereas, W. Mann, J.C. Miller, 1961. Experiments in preserving and revitalizing pine, onion, and okra seeds. Economic Bot. 15: 213-217.
Tammela, P., P. Salo-Vänänen, I. Laakso, A. Hopia, H. Vuorela, M. Nygren, 2005. Tocopherols, tocotrienols and fatty acids as indicators of natural ageing in Pinus sylvestris seeds. Scand. J. For. Res. 20: 378-384.
Vollhardt, K.P., N.E. Schore, 2004. Organic Chemistry: structure and function. Fourth Edition. New York:W.H. Freeman and Company.
Zacheo, G., A.R. Cappello, L.M. Perrone, G.V. Gnoni, 1998. Analysis of factors influencing lipid oxidation of almond seeds during accelerated ageing. LWT – Food Sci. Technol. 31: 6-9.
Zalewski K., D. Widejko, R.J. Górecki, 2000. The influence of CO2, temperature and α-tocopherol on phospholipid changes in embryonic axes of field bean seeds during storage. Acta Soc. Bot. Pol 69: 123-126.