Emre İLKER, Muzaffer TOSUN, Fatma Aykut TONK

A sutudy to incorporate high protein content from tetraploid wheat (T. turgidum dicoccoides) to hexaploid wheat (T. aestivum vulgare)

Grain protein content of wheat is important for bread making and pasta quality. Wild tetraploid wheat (Triticum turgidum L. var. dicoccoides) has some genes for high protein content as a promising source. Three winter wheat cultivars of Colorado (Halt, Yuma, Akron) and one experimental line (CO99508) and one accession of T. dicoccoides were crossed to obtain backcrosses. The objective of this study was to determine higher protein content, SDSsedimentation and hardiness values in backcrosses T. aestivum x T. dicoccoides. Analysis of variance showed that highly significant differences were found among parents and backcrosses. The minimum and maximum values of grain protein content of all backcross populations changed from low protein parents (bread wheat cultivars) to high protein parent (T. dicoccoides). This increase in grain protein content of backcrosses is most likely due to the transferring of the high protein genes from the T. dicoccoides to the hexaploid cultivars. Although T. dicoccoides has high grain protein content, bread making quality of T. dicoccoides has lower than bread wheat cultivars. This situation has also been observed in all backcross populations. However, based on the results of this study, it could be concluded that high protein genes in the wild tetraploid wheat (T. dicoccoides) could possibly be transferred to the bread wheat cultivars.

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Avivi, L., 1978. High grain protein content in wild tetraploid wheat Triticum dicoccoides Korn. In: Ramanujan S. (ed.), 5th Int. Wheat Genet. Symp., pp. 372-380. New Delhi, India.
Belderok, B., 2000. Developments in bread-making processes. In: Belderok, B., Mesdag, J., Donner D.A. (eds), Bread Making Quality of Wheat: A Century of Breeding in Europe. ISBN 0-7923-6383-3. Kluwer Academic Publishers, Netherlands.
Chee, P.W., E.M. Elias, J.A. Anderson, S.F. Kianian, 2001. Evaluation of a high protein QTL from Triticum turgidum L. var. dicoccoides in an adapted durum wheat background. Crop Sci. 41:295-301.
Gonzalez-Hernandez, J.L., E.M. Elias, S.F. Kianian, 2004. Mapping genes for grain protein concentration and grain yield on chromosome 5B of Triticum turgidum (L.) var. dicoccoides. Euphytica 139:217-225.
Graybosch, R.A., 2000. Improved quality of 1RS wheats via genetics and breeding. In: P.R. Shewry P.R., Tatham A.R. (eds), Gluten 2000. 7th Int. Workshop on Gluten Proteins, 2-6 April 2000, Inst. Arable Crops Res. Bristol, UK.
Groos, C., N. Robert, E. Bervas, G. Charmet, 2003. Genetic analysis of grain protein-content, grain yield and thousand-kernel weight in bread wheat. Theor. Appl. Genet. 106:1032-1040.
Joppa, L.R., R.G. Cantrell, 1990. Choromosomal location of genes for grain protein content in wild tetraploid wheat. Crop Sci. 30:1059-1064.
Joppa, L.R., C. Du, G.E. Hart, G.A. Hareland, 1997. Mapping QTL for grain protein in tetrploid wheat (Triticum turgidum L.) using a population of recombinant inbred chromosome lines. Crop Sci. 37:1586-1589.
Khan, I.A., J.D. Procunier, D.G. Humpreys, G. Tranquilli, A.R. Schlatter, S. Marcucci-Poltri, R. Frohberg, J. Dubcovsky, 2000. Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Sci. 40:518-524.
Kunert, A., A.A. Naz, O. Dedeck, K. Pillen, J. Leon, 2007. AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides T. tauschii) as a source of favourable alleles for milling and baking quality traits. Theor. Appl. Genet. 115:683-695.
Kushnir, U., G.M. Halloran, 1984. Transfer of high kernel weight and high protein from wild tetraploid wheat (T. turgidum dicoccoides) to bread wheat (T. aestivum) using homologous and homoeologous recombination. Euphytica 33:249- 255.
Law, C.N., C.F. Young, J.W.S. Brown, J.W. Snape, J.W. Worland, 1978. The study of grain protein control in wheat using whole chromosome substitution lines. In: Seeds Protein Improvement by Nuclear Techniques. International Atomic Energy Agency, Vienna, Austria, pp. 483-502.
Ruiz, M., J.M. Carrillo, 1995. Relationships between different prolamin proteins and some quality properties in durum wheat. Plant Breed. 114:40-44.
Pena, R.J., 2002. Wheat for bread and other foods. In: Curtis, B.C., Rajaram, S., Macpherson, H.G. (eds), Bread Wheat Improvement and Production. FAO Plant production and protection series, No:30, Roma.
Steel, R.G.D., J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2. ed. New York: Mc Graw- Hill, 631p.
Steiger, D.K., E.M. Elias, R.G. Cantrell, 1996. Evaluation of lines derived from wild emmer chromosome substitutions, I. Quality traits. Crop Sci. 36:223-227.
Zanetti, S., M. Winzeler, C. Feuillet, B. Keller, M. Messmer, 2001. Genetic analysis of bread-making quality in wheat and spelt. Plant Breed. 120:13-19.