A preliminary study for identification of candidate eflp markers for leaf rust resistance gene Lr13by using near isogenic lines of spring wheat cv. Thatcher

The aim of the present study was to detect candidate DNA markers for leaf rust resistance gene Lr13. The NIL groups were constructed from a set of near isogenic lines of wheat cv. Thatcher containing in total 41 different Lr resistance genes. A total number of 246 primer combinations in the NIL groups were screened for DNA polymorphism by using the AFLP method. Twenty-eight AFLP primer combinations yielded 33 polymorphic bands in Tc+Lr13 line. To confirm these polymorphic bands, 28 primer combinations were investigated in 41 Thatcher near isogenic lines. Six primer combinations amplified specific band products in Tc+Lr13. It was concluded that these AFLP markers can be linked to Lr13 gene and in further studies on Lr13 resistance gene, these six AFLP markers should be analyzed in near isogenic lines. Also, construction of the NIL groups can be useful in terms of time and cost of the molecular researches including Thatcher near isogenic lines.

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