Chalermpol PHUMICHAI,
Weerachai MATTHAYATTHAWORN,
Nipha CHUENPOM,
Arunee WONGKAEW,
Phakaked SOMSAENG,
Tanapong YODYINGYONG,
Pherawich PANKLANG,
Sujin JENWEERAWAT,
Yaowamarn KEAWSAARD,
Thitaporn PHUMICHAI,
Tanee SREEWONGCHAI,
Rangsarid KAVEETA
13130
IDENTIFICATION OF A SCAR MARKER LINKED TO A SHATTERING RESISTANCE TRAIT IN SESAME
Sesame (Sesamum indicum L.) is one of the most important oil crops in temperate and tropical regions and is grown worldwide over an area of 5179 (hg ha-1 ) to produce 5.469.024 tonnes of seed. Capsule shattering before or during harvest can cause yield losses of greater than 50%. The objectives of the present study were to evaluate the inheritance of resistance to capsule shattering in the F2 derived from a cross between Cplus1, a sesame line with shattering-resistant capsules (Sh1Sh1Sh2Sh2), and KUAOX25, a line with shatteringsusceptible capsules (sh1sh1sh2sh2- ); and use bulked segregant analysis and Amplified Fragment Length Polymorphisms (AFLPs) to identify Sequence Characterized Amplified Region (SCAR) markers associated with shattering resistance. After screening 192 AFLP primer combinations, nine polymorphic bands were identified, and one of these AFLPs was developed into a Si-SR-32-19 SCAR that could distinguish between shattering-susceptible and shattering-resistant phenotypes.
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- Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang,
W. Miller and D.J. Lipman. 1997. Gapped BLAST and
PSIBLAST: A new generation of protein database search
programs. Nucleic Acids Res. 25: 3389–3402.
- Anilakumar, K.R., A. Pal, F. Khanum, and A.S. Bawa. 2010.
Nutritional, medicinal and industrial uses of sesame
(Sesamum indicum L.) seeds: An overview. Agric. Conspec.
Sci. 75:159–168
- Arslan, C., B. Uzun, S. Ulger and M.I. Cagirgan. 2007.
Determination of oil content and fatty acid composition of
sesame mutants suited for intensive management conditions.
J Am Oil Chem Soc. 84(10): 917-920.
- Ashri, A. 1998. Sesame breeding. Plant Breed. Rev. 16:179–228.
Ashri, A. 2010. Sesame breeding. In: Plant Breed Rev., ed.
Janick, J., Volume 16, Wiley, Oxford.
- Ashutosh, A., P.C. Sharma, S. Prakash and S.R. Bhat. 2007.
Identification of AFLP markers linked to the male sterility
restorer gene of CMS (Moricandia arvensis) Brassica juncea
and conversion to SCAR markers. Theor. Appl. Genet. 114:
385–392.
- Benbouza, H., J.M. Jacquemin, J.P. Baudoin and G. Mergeai.
2006. Optimization of a reliable, fast, cheap and sensitive
silver staining method to detect SSR markers in
polyacrylamide gels. Biotechnol. Agron. Soc. Environ. 10:
77–81.
- Cagirgan, M.I. 1996. Radiosensitivity of Turkish sesame to
gamma-rays. Turk.J. Field Crops 1: 39–43.
- Cagirgan, M.I. 2001. Mutation techniques in sesame (Sesamum
indicum L.) for intensive management: confirmed mutants.
In: Sesame Improvement by Induced Mutations. Final report
of an FAO/IAEA co-ordinated research project organized by
the Joint FAO/IAEA Division of Nuclear Techniques in
Food and Agriculture. 31–40. International Atomic Energy
Agency (IAEA).
- Cagirgan, M.I. 2006. Selection and morphological
characterization of determinate mutants in sesame. Field
Crops Res. 96:19–24.
- Cagirgan, M.I., S. Ozerden and M.O. Ozbas. 2009. Agronomic
trait assessment and selection for number of capsules in
determinate x indeterminate crosses of sesame. Turk J Agric
For. 33: 231–241.
- Cosio, C. and C. Dunand. 2010. Transcriptome analysis of
various flower and silique development stages indicates a set
of class III peroxidase genes potentially involved in pod
shattering in Arabidopsis thaliana. BMC Genomics 11: 528.
- Dussle, C.M., M. Quint, A.E. Melchinger, M.L. Xu and T.
Lübberstedt. 2003. Saturation of two chromosome regions
conferring resistance to SCMV with SSR and AFLP markers
by targeted BSA. Theor. Appl. Genet. 106: 485–493.
- FAOSTAT. 2014. FAOSTATdatabase. FAO,Rome,Italy.
http://faostat3.fao.org/browse/Q/QC/E, (Accessed July 25,
2016)
- Ferrandiz, C., S.J. Liljegren and M.F. Yanofsky.2000. Negative
regulation of the SHATTERPROOF genes by FRUITFULL
during Arabidopsis fruit development Science 289: 436–438.
- Girin, T., P. Stephenson, C.M.P. Goldsack, S.A. Kempin, A.
Perez, N. Pires, P.A. Sparrow, T.A. Wood, M.F. Yanofsky
and L. Østergaard. 2010. Brassicaceae INDEHISCENT genes
specify valve margin cell fate and repress replum formation.
Plant J. 63: 329–338.
- Hayashi, M., A. Ujiie, H. Serizawa, H. Sassa, H. Kakui, T. Oda
and T. Koba. 2011. Development of SCAR and CAPS
markers linked to a recessive male sterility gene in lettuce
(Lactuca sativa L.). Euphytica 180: 429–436.
- Jenkins, E.S., W. Paul, M. Craze, C.A. Whitelaw, A. Weigand
and J.A. Roberts. 1999. Dehiscence-related expression of an
Arabidopsis thaliana gene encoding a polygalacturonase in
transgenic plants of Brassica napus. Plant Cell Environ. 22:
159–167.
- Ke, L.P., Y.Q. Sun, P.W. Liu and G.S. Yang. 2004.
Identification of AFLP fragments linked to one recessive
genic male sterility (RGMS) in rapeseed (Brassica napus L.)
and conversion to SCAR markers for marker-aided selection.
Euphytica 138: 163–168.
- Ke, T., C. Dong, H. Mao, Y. Zhao, H. Chen, H. Liu, X. Dong, C.
Tong and S. Liu. 2011. Analysis of expression sequence
tags from a full-length-enriched cDNA library of developing
sesame seeds (Sesamum indicum). BMC Plant Biol. 11: 180.
- Kotcha, A., W. Wongyai, P. Pongtongkam and J. Verawudh.
2012. Inheritance of shatter resistance capsules in sesame.
Thai J. Genet. 5:149–158 (in Thai).
- Langham, D.G. 1946. Genetics of sesame III: “open sesame” and
mottled leaf. J. Hered. 37: 149–152.
- Langham, D.G. and M. Rodriguez. 1946. Abrete 265esame:
Ajonjoli (Sesamum indicum) que no pierde semillas. Circ.
17, Publ. Ministerio de Agricultura y Cria, Maracay,
Venezuela.
- Langham, D.G., M. Rodriguez and E. Reveron. 1956.
Dehiscencia, y otras caracteristicas del ajonjoli Sesamum
indicum L., en relacion con el problema de la cosecha.
Genesa, Publ. Tecnica 1, Maracay, Venezuela.
- Langham, D.R. 2001. Shatter resistance i192 AFLP primer
combination n sesame. p. 51–61. In: L. Van Zanten (ed.),
Sesame improvements by induced mutations, Proc. Final
FAO/IAEA Co-ord. Res. Mtng, IAEA, Vienna, TECDOC-
1195.
- Langham, D. R. and T. Wiemers. 2002. Progress in mechanizing
sesame in the US through breeding. In: Trends in New Crops
and New Uses, eds. Janick, J. and Whipkey, A., 157–173,
ASHS Press, Alexandria.
- Lenser, T. and G. Theißen. 2013. Conservation of fruit
dehiscence pathways between
Lepidium campestre and Arabidopsis thaliana sheds light on the
regulation of INDEHISCENT. Plant J. 76:545–556.
- Liljegren, S.J., A.H. Roeder, S.A. Kempin, K. Gremski, L.
Østergaard, S. Guimil, D.K. Reyes and M.F. Yanofsky.
2004. Control of fruit patterning in Arabidopsis by
INDEHISCENT. Cell 19:843–853.
- Liljegren, S.J., G.S. Ditta, Y. Eshed, B. Savidge, J.L. Bowman
and M.F. Yanofsky. 2000. SHATTERPROOF MADS-box
genes control seed dispersal in Arabidopsis. Nature 404:766–
770.
- Michelmore, R.W., I. Paran and R.V. Kesseli. 1991.
Identification of markers linked to disease-resistance genes
by bulked segregant analysis: a rapid method to detect
markers in specific genomic regions by using segregating
populations. Proc. Natl. Acad. Sci. 88: 9828–9832.
- Microsoft. 2010. Excel. Microsoft Office
2010. Microsoft, Redmond, WA.
- Mitsuda, N., A. Iwase, H. Yamamoto, M. Yoshida, M. Seki, K.
Shinozaki and M. Ohme-takagi. 2007. NAC transcription
factors, NST1 and NST3, are key regulators of the formation
of secondary walls in woody tissues of Arabidopsis. Plant
Cell. 19: 270–280.
- Negi, M.S., M. Devic, M. Delseny and M. Lakshmikumaran.
2000. Identification of AFLP fragments linked to seed coat
colour in Brassica juncea and conversion to a SCAR marker
for rapid selection. Theor. Appl. Genet. 101:146–152.
Ogawa, M., P. Kay, S. Wilson and S.M. Swain. 2009.
- Arabidopsis dehiscence zone polygalacturonase1 (ADPG1),
ADPG2, and QUARTET2 are polygalacturonases required
for cell separation during reproductive development in
Arabidopsis. Plant Cell. 21:216–233.
- Østergaard, L., S.A. Kempin, D. Bies, H.J. Klee and M.F.
Yanofsky. 2006. Pod shatter resistant Brassica fruit
produced by ectopic expression of the FRUITFULL gene.
Plant Biotech. J. 4:45–51.
- Rajani, S. and V. Sundaresan. 2001. The Arabidopsis myc/bHLH
gene ALCATRAZ enables cell separation in fruit dehiscence.
Curr. Biol. 11:1914–1922.
- Rozen, S. and H. Skaletsky. 2000. Primer3 on the www for
general users and for biologist programmers. Methods of
Molecular Biology 132: 365–386.
- Silme, R.S. and M.I. Cagirgan. 2010. Screening for resifigstance
to fusarium wilt in induced mutants and world collection of
sesame under intensive management. Turkish Journal of
Field Crops 15:89–93.
- Tan, X., Z. Xia, L. Zhang, Z. Zhang, Z. Guo and C. Qi. 2009.
Cloning and sequence analysis of oilseed rape (Brassica
napus) SHP2 gene. Bot. Stud. 50:403–412.
- Tashiro, T., Y. Fukuda, T. Osawa and M. Namiki. 1990. Oil and
minor components of sesame (Sesamum indicum L.) strains.
J. Am. Oil Chem. Soc. 67:508–511.
- Vos, P., R. Hogers, M. Bleeker, M. Reijans, T.V. de Lee, M.
Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M.
Zabeau. 1995. AFLP: a new technique for DNA
fingerprinting. Nucleic Acids Res. 23:4407–4414.
- Wongyai, W. and S. Chowchong. 2003. C plus 1: the new white
sesame variety with non-shattering. Proceedings of the 3rd
National Conference on Sesame, Sunflower, Castor and
Safflower, Chiang Mai, Thailand (in Thai).