THE FLORAL AND SCENT CHARACTERISTICS OF LAVANDIN FROM THE MICROPROPAGATION AND CUTTING PROPAGATION

Lavandin (Lavandula x intermedia Emeric ex Loisel.) is one of the most valuable species for the fragrance industries due to its extraordinary scent. This research was carried out to compare the floral and scent characteristics from the micropropagation and cutting propagation in field conditions for four years between 2014 and 2017 in Isparta-a city in the south-western part of Turkey, which is called “The Lavender Valley of Turkey”. The experiment was arranged according to a randomized complete-block design with three replications. In comparison with the propagation methods, the yield and floral characteristics of the micropropagation were higher than the cutting propagation. The fresh stem flower yield, the dried flower yield, and the examined characteristics of the lavandin were higher in the subsequent years compared to the previous years. While the highest plant height (90.10 cm), the spike length (10.16 cm), the leaf length (56.16 mm), the spike number per plant (479.0 spike plant-1), the cluster number per spike (10.70 cluster spike-1), the fresh stem flower yield (7791.0 kg ha-1), and the dried flower yield (2054.6 kg ha-1) were obtained from the micropropagation method in the year 2017, the lowest values of these characteristics were determined from the cutting propagation in 2014. The essential oil contents of the lavandin were not significantly different in both propagation methods. The essential oil contents of fresh stem flower and the dried flower of lavandin were decreased in the subsequent years, and the highest essential oil content (1.27 and 7.36%, respectively) was determined from 2014, and the lowest essential oil content (1.21 and 6.92%, respectively) from 2017. The linalool, linalyl acetate, camphor, α-terpineol, and borneol L were close to each other in both propagation methods, while the eucalyptol content of the micropropagation was higher than the cutting propagation.

Keywords:

Essential oil, lavandin micropropagation, yield,

PDF

___

Andrade, L.B., S. Echeverrigaray, F. Fracaro, G.F. Pauletti and L. Rota. 1999. The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera D.C.). Plant Cell, Tissue and Organ Culture 56 (2): 79-83.
Arabaci, O. and E. Bayram. 2005. The effect of nitrogen fertilization and plant density on some agronomic and quality traits of lavender (Lavandula angustifolia Mill.) under ecological conditions of Aydın. Adnan Menderes University Journal of Agriculture Faculty 2(2): 13-19 (in Turkish).
Bajaj, Y.P.S., M. Furmanowa and O. Olszowska. 1988. Biotechnology of the micropropagation of medicinal and aromatic plants. Biotechnology in Agriculture and Forestry 4(2): 60-103.
Baydar, H. and S. Erbas. 2007. Effects of harvest time and drying on essential oil properties in lavandin (Lavandula x intermedia Emeric ex Loisel.). I. International Medicinal and Aromatic Plants Conference on Culinary Herbs, pp. 377-381, 29 April-4 May 2007, Antalya-Turkey.
Boeckelmann, A. 2008. Monoterpene production and regulation in lavenders (Lavandula angustifolia and Lavandula x intermedia). The Master Thesis, University of British Columbia, 98p.
Calvo, M.C. and J. Segura. 1989. Plant regeneration from cultured leaves of Lavandula latifolia Medicus: Influence of growth regulators and illumination conditions. Plant Cell, Tissue and Organ Culture 19(1): 33-42.
Canli, F.A. and L. Tian. 2008. In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars. Scientia Horticulturae 116(1): 34-40.
Chambon, C., A. Poupet, D. Beck, B. Bettachini and J. Touche. 1992. In vitro morphogenetic potential of various lavandin and lavender clones: Preliminary observations on the agronomic value of the vitroplants. Agronomie (Paris) 12(1): 173-181.
Chawla, H.S. 2009. Introduction to Plant Biotechnology, 3rd ed. Science Publishers, Enfield, NH, USA.
Dias, M.C., R. Almeida and A. Romano. 2002. Rapid clonal multiplication of Lavandula viridis through in vitro axillary shoot proliferation. Plant Cell, Tissue and Organ Culture 68(1): 99-102.
Degenhardt, J., T.G. Koellner and J. Gershenzon. 2009. Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants. Phytochemistry 70(15-16): 1621-37.
Echeverrigaray, E., R. Basso and L.B. Andrade. 2005. Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants. Biologia Plantarum 49(3): 439-442.
Ganjewala, D., A. Kumari and K.H. Khan. 2008. Ontogenic and developmental changes in essential oil content and compositions in Cymbopogon flexuosus cultivars. In Prasad BN, Lazer Mathew, eds. Recent Advance in Biotechnology. Excel India Publishers, New Delhi, India, pp. 82-92.
Giorgi, A., S. Panseri, M.S. Mattara, C. Andreis and L.M. Chiesa. 2013. Secondary metabolites and antioxidant capacities of Waldheimia glabra (Decne.) Regel from Nepal. Journal Science Food Agriculture 93(5): 1026-1034.
Gonçalves, S., H. Serra, J.M.F. Nogueira, R. Almeida, L. Custodio, L. and A. Romano. 2008. Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis. Biologia Plantarum 52(1): 133-136.
Gupta, A.K. and D. Ganjewala. 2015. A study on developmental changes in essential oil content and composition in Cymbopogon flexuosus cultivar. Acta Biologica Szegediensis 59(2): 119-125.
Hutchinson, J.F. and R.H. Zimmerhan. 1987. Tissue culture of temperate fruit and nut trees. Horticultural Reviews 9(2): 273-349.
Kara, N. and H. Baydar. 2013. Determination of lavender and lavandin cultivars (Lavandula sp.) containing high quality essential oil in Isparta, Turkey. Turkish Journal of Field Crops 18(1): 58-65.
Kassaye, E. and B.D. Bekele. 2015. In vitro optimization of the protocol for micropropagation of plum (Prunus salicina L. Var. Methley) from nodal explants. Biotechnology International 8(4): 137-148.
Lakusic, B.S., M.S. Ristic, V.N. Slavkovska, D.L. Stojanovic and D.V. Lakusic. 2013. Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. Botanica Serbica 37(2): 127-139.
Lima, V.A., F.V. Pacheco, R.P. Avelar, I.C.A. Alvarenga, J.E.B.P. Pinto, A.A. De Alvarenga. 2017. Growth, photosynthetic pigments and production of essential oil of long-pepper under different light conditions. Annals of the Brazilian Academy of Science 89(2): 1167-1174. https://doi.org/10.1590/0001-3765201720150770
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tabocco tissue culture. Physiology Plantarum 15: 473-497.
Nogueira, J.M.F. and A. Romano. 2002. Essential oils from micropropagated plants of Lavandula viridis. Phytochemical Analysis 13(1): 4-7.
Ozturk, G. and Z. Yildirim. 2010. A comparision of field performances of minitubers and microtubers used in seed potato production. Turkish Journal of Field Crops 15(2): 141-147. Ozturk, G. and Z. Yildirim. 2011. Uniformity of potato minitubers derived from meristem cultures of nuclear seed stocks. Turkish Journal of Field Crops 16(2): 149-152.
Paez, A., G.M. Gebre, M.E. Gonzalez and T.J. Tschaplinski. 2000. Growth, soluble carbohydrates, and aloin concentration of Aloe vera plants exposed to three irradiance levels. Environmental Experimental Botany 44(2): 133-139.
Pierik, R.L.M. 1987. In vitro Culture of Higher Plants. Martinus Nijhoff Publishers, Dordrect 344p.
Quazi, M.H. 1980. In vitro propagation of Lavandula spp. Annals Botany 45: 361-362.
Sarker, L.S. 2013. Cloning of Lavandula essential oil biosynthetic genes. The Master Thesis, The University of British Columbia, 111p.
Segura, J. and M.C. Calvo. 1991. Lavandula spp. (Lavender): In vitro culture, regeneration of plants and formation of essential oils and pigments. Biotechnology in Agriculture and Forestry 15:283-310.
Sonmez, C., A.O.S Soysal, H. Okkaoglu, U. Karik, A.H. Taghiloofar and E. Bayram. 2018. Determination of some yield and quality characteristics among individual plants of lavender (Lavandula angustıfolıa MILL.) populations grown under Mediterranean conditions in Turkey. Pakistan Journal of Botany 50(6): 2285-2290.
Stainier, C. 1975. Role and functions of the European Pharmacopoeia. Ann Ist Super Sanita 11(3-4): 211-219.
Steel, R.G.D. and J.H. Torrie. 1980. Principles and Procedures of Statistics. 2nd edition, McGraw-Hill Book Co. New York. Stein, S.E. 1990. National Institute of Standards and Technology (NIST) Mass Spectral Database and Software, Version 3.02 USA.
Sudria, C., M.T. Pinol, J. Palazon, R.M. Cusido, R. Vila, C. Morales, M. Bonfill and S. Canigueral. 1999. Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentate plantlets. Plant Cell, Tissue and Organ Culture 58(3): 177-184.
Wang, X., L. Jin., M. Li, M. Zhao, H. Zhao, Y. Xu. 2007. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'. Journal of Horticultural Science and Biotechnology 82(5):781-785.
Zuzarte, M., A.M. Dinis, C.C. Ligia, R. Salgueiro and J. Canhoto. 2010. Trichomes, essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae). Industrial Crops and Products 32(3): 580-587.

ISSN: 1301-1111
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 1996
Yayıncı: Tarla Bitkileri Bilimi Derneği

Arşiv