Synthesis of novel SN38-aspirin pro drugs for the treatment of hepato cellular carcinoma
Synthesis of novel SN38-aspirin pro drugs for the treatment of hepato cellular carcinoma
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is a leading cause ofcancer-related death worldwide. However, there is no effective chemotherapeutic treatment for HCC and its prognosisremains poor. Consequently, it is urgent to find an efficient antitumor agent to treat HCC. In this study, 7-ethyl-10-hydroxycamptothecin (SN38), the bioactive metabolite of the anticancer drug irinotecan (CPT-11), which is 100–1000times more potent than CPT-11, was coupled with aspirin to give 4 prodrugs. Their structures were characterized by1 H NMR and elemental analysis. The in vitro anticancer activities of these compounds on two human hepatocellularcarcinoma cell lines (BEL-7404 and HepG2) and preliminary mechanisms of action were explored. Our data indicatedthat these compounds decreased the viability of cancer cell lines in a concentration- and time-dependent manner. Amongthem, compound 4b significantly inhibited cell viability of HepG2 cells (IC 50 = 0.1208 µ M) when compared with CPT-11 (IC 50 = 18.4267 µ M). Furthermore, compound 4b blocked HepG2 cell migration and invasion in vitro. These findingssuggest that compound 4b may be used as a promising anticancer agent against HCC.
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- 1. Ferlay, J.; Soerjomataram, I.; Dikshit, R.; Eser, S.; Mathers, C.; Rebelo, M.; Parkin, D. M.; Forman, D.; Bray, F. Int. J. Cancer 2015, 136, E359-386.
- 2. Siegel, R. L.; Miller, K. D.; Jemal, A. CA Cancer J. Clin. 2016, 66, 7-30.
- 3. Giannini, E. G.; Farinati, F.; Ciccarese, F.; Pecorelli, A.; Rapaccini, G. L.; Di Marco, M.; Benvegn`u, L.; Caturelli, E.; Zoli, M.; Borzio, F. et al. Hepatology 2015, 61, 184-190.
- 4. Stintzing, S.; Modest, D. P.; Rossius, L.; Lerch, M. M.; von Weikersthal, L. F.; Decker, T.; Kiani, A.; Vehling-Kaiser, U.; Al-Batran, S. E.; Heintges, T. et al. Lancet Oncol. 2016, 17, 1426-1434.
- 5. Noda, K.; Nishiwaki, Y.; Kawahara, M.; Negoro, S.; Sugiura, T.; Yokoyama, A.; Fukuoka, M.; Mori, K.; Watanabe, K.; Tamura, T. et al. N. Engl. J. Med. 2002, 346, 85-91.
- 6. Boku, N.; Yamamoto, S.; Fukuda, H.; Shirao, K.; Doi, T.; Sawaki, A.; Koizumi, W.; Saito, H.; Yamaguchi, K.; Takiuchi, H. et al. Lancet Oncol. 2009, 10, 1063-1069.
- 7. Sugiyama, T.; Okamoto, A.; Enomoto, T.; Hamano, T.; Aotani, E.; Terao, Y.; Suzuki, N.; Mikami, M.; Yaegashi, N.; Kato, K. et al. J. Clin. Oncol. 2016, 34, 2881-3887.
- 8. Jansen, W. J.; Zwart, B.; Hulscher, S. T.; Giaccone, G.; Pinedo, H. M.; Boven, E. Int. J. Cancer 1997, 70, 335-340.
- 9. Matsuoka, H.; Yano, K.; Seo, Y.; Saito, T.; Tomoda, H.; Takiguchi, S.; Kono, A. Anticancer Drugs 1995, 6, 413-418.
- 10. Takahata, T.; Ookawa. K.; Suto, K.; Tanaka, M.; Yano, H.; Nakashima, O.; Kojiro, M.; Tamura, Y.; Tateishi, T. Sakata, Y. et al. Basic Clin. Pharmacol. Toxicol. 2008, 102, 399-407.
- 11. Mathijssen, R. H.; van Alphen, R. J.; Verweij, J.; Loos, W. J.; Nooter, K.; Stoter, G.; Sparreboom, A. Clin. Cancer Res. 2001, 7, 2182-2194.
- 12. O’Reilly, E. M.; Stuart, K. E.; Sanz-Altamira, P. M.; Schwartz, G. K.; Steger, C. M.; Raeburn, L.; Kemeny, N. E.; Kelsen, D. P.; Saltz, L. B. Cancer 2011, 91, 101-105.
- 13. Boige, V.; Ta¨ıeb, J.; Hebbar, M.; Malka, D.; Debaere, T.; Hannoun, L.; Magherini, E.; Mignard, D.; Poynard, T.; Ducreux, M. Eur. J. Cancer 2006, 42, 456-459.
- 14. Sadzuka, Y.; Takabe, H.; Sonobe, T. J. Control. Release 2005, 108, 453-459.
- 15. Yao, Y.; Su, X.; Xie, Y.; Wang, Y.; Kang, T.; Gou, L.; Yi, C.; Yang, J. Anticancer Drugs 2013, 24, 270-277.
- 16. Tahara, M.; Inoue, T.; Sato, F.; Miyakura, Y.; Horie, H.; Yasuda, Y.; Fujii, H.; Kotake, K.; Sugano, K. Mol. Cancer Ther. 2014, 13, 1170-1180.
- 17. Yao, Y.; Yu, L.; Su, X.; Wang, Y.; Li, W.; Wu, Y.; Cheng, X.; Zhang, H.; Wei, X.; Chen, H. et al. J. Control. Release 2015, 220, 5-17.
- 18. Santi, D. V.; Schneider, E. L.; Ashley, G. W. J. Med. Chem. 2014, 57, 2303-2314.
- 19. Scarpignato, C.; Hunt, R. H. Gastroenterol. Clin. North Am. 2010, 39, 433-464.
- 20. Thorat, M. A.; Cuzick, J. Curr. Oncol. Rep. 2013, 15, 533-540.
- 21. Thun, M. J.; Jacobs, E. J.; Patrono, C. Nat. Rev. Clin. Oncol. 2012, 9, 259-267.
- 22. Sahasrabuddhe, V. V.; Gunja, M. Z.; Graubard, B.; Trabert, B.; Schwartz, L. M.; Park, Y.; Hollenbeck, A. R.; Freedman, N. D.; McGlynn, K. A. J. Natl. Cancer Inst. 2012, 104, 1808-1814.
- 23. Hossain, M. A.; Kim, D. H.; Jang, J. Y.; Kang, K. J.; Yoon, J. H.; Moon, J. O.; Chung, H. Y.; Kim, G. Y.; Choi, Y. H.; Copple, B. L. et al. Int. J. Oncol. 2012, 40, 1298-1304.
- 24. Li, T.; Dong, Z. R.; Guo, Z. Y.; Wang, C. H.; Tang, Z. Y.; Qu, S. F.; Chen, Z. T.; Li, X. W.; Zhi, X. T. Cancer Gene Ther. 2013, 20, 366-374.
- 25. Tewari, D.; Majumdar, D.; Vallabhaneni, S.; Bera, A. K. Sci. Rep. 2017, 7, 45184.
- 26. Li, G.; Zhang, S.; Fang, H.; Yan, B.; Zhao, Y.; Feng, L.; Ma, X.; Ye, X. Biochem. Biophys. Res. Commun. 2013, 434, 809-814.
- 27. Li, S.; Dai, W.; Mo, W.; Li, J.; Feng, J.; Wu, L.; Liu, T.; Yu, Q.; Xu, S.; Wang, W. et al. Int. J. Cancer 2017, 141, 2571-2584.
- 28. Abbas, I.; Gomha, S.; Elaasser, M.; Bauomi, M. Turk. J. Chem. 2015, 39, 334-346.
- 29. Hassan, A. S.; Hafez, T. S.; Osman, S. A. M.; Ali, M. M. Turk. J. Chem. 2015, 39, 1102-1113.
- 30. Wang, Y. H.; Sui, X. M.; Sui, Y. N.; Zhu, Q. W.; Yan, K.; Wang, L. S.; Wang, F.; Zhou, J. H. Oncol. Lett. 2015, 10, 2227-2232.
- 31. Wang, T.; Xuan, X.; Li, M.; Gao, P.; Zheng, Y.; Zang, W.; Zhao, G. Diagn. Pathol. 2013, 8, 179.
- 32. Friedl, P.; Wolf, K. Nat. Rev. Cancer 2003, 3, 362-374.
- 33. Yang, F.; Hu, M.; Lei, Q.; Xia, Y.; Zhu, Y.; Song, X.; Li, Y.; Jie, H.; Liu, C.; Xiong, Y. et al. Cell Death Dis. 2015, 6, e1701.
- 34. Li, Y.; Liu, B.; Yang, F.; Yu, Y.; Zeng, A.; Ye, T.; Yin; W.; Xie, Y.; Fu, Z.; Zhao, C. Biomed. Pharmacother 2016, 83, 1239-1246.