Secondary metabolites from Sambucus ebulus
A new nonglycosidic iridoid, sambulin B (1), was isolated from the methanol extract of Sambucus ebulus L. leaves along with a recently reported new nonglycosidic iridoid, 10-O-acetylpatrinoside aglycone (sambulin A) (2); 2 flavonoids, isorhamnetin-3-O-b-D-glucopyranoside (3) and isorhamnetin-3-O-rutinoside (4); and a mixture of 2 flavonoids (5), quercetin-3-O-b-D-glucopyranoside and quercetin-3-O-b-D-galactopyranoside. Their structures were elucidated by 1-D and 2-D nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) experiments.
Secondary metabolites from Sambucus ebulus
A new nonglycosidic iridoid, sambulin B (1), was isolated from the methanol extract of Sambucus ebulus L. leaves along with a recently reported new nonglycosidic iridoid, 10-O-acetylpatrinoside aglycone (sambulin A) (2); 2 flavonoids, isorhamnetin-3-O-b-D-glucopyranoside (3) and isorhamnetin-3-O-rutinoside (4); and a mixture of 2 flavonoids (5), quercetin-3-O-b-D-glucopyranoside and quercetin-3-O-b-D-galactopyranoside. Their structures were elucidated by 1-D and 2-D nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) experiments.
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- –6 ( c = 0.33, CHCl3) ; UV (CHCl3) : λ max 241, 274, 282; IR (KBr) υmaxcm −1 : 3418, 1736, 1372, 1235;
- ESI-MS: m/z = 343 [M+H]+,1H NMR (600 MHz, CDCl3) and13C NMR (150 MHz, CDCl3) : Table 1. Isorhamnetin-3-O - β -D-glucopyranoside (3): Yellow amorphous powder; C22H22O12,UV (MeOH): λmax264, 354 nm, IR (KBr) υmaxcm −1 : 3409, 1653, 1607, HR-ESI-MS: m/z = 501.1003 [M+Na]+,1H NMR
- (600 MHz, CD3OD) and13C NMR (150 MHz, CD3OD) Table 2. Isorhamnetin-3-O -rutinoside (4): Yellow amorphous powder; C28H32O16, UV (MeOH): λmax266, 354 nm, IR (KBr) υmaxcm −1 : 3452, 2934, 1665, 1608, HR-ESI-MS: m/z = 647.1583 [M+Na]+,1H NMR (600
- MHz, CDOD) and13C NMR (150 MHz, CD3OD): Table 2. Mixture of isoquercitrin (5a) and hyperoside (5b) (2/3) (5): Yellow amorphous powder; UV (MeOH): λmax266, 352 nm, IR (KBr) υmaxcm −1 : 3415, 1655, 1605, HR-ESI-MS: m/z = 487.0847 [M+Na]+, 1H NMR (600 MHz, CDOD) and13C NMR (150 MHz, CDOD): Table 3. 3OD) and 3OD): Table 3.