M. Said NUTKU, F. Bedia ERİM BERKER

6980

Polyethyleneimine-Coated Capillaries for the Separation of DNA by Capillary Electrophoresis

A new capillary electrophoresis of DNA fragments has been achieved with the cooperative effects of the electrophoretic and electroosmotic mobilities realized via the polyethyleneimine (PEI) coating of the capillary walls. As a result, seven of the 11 different-sized DNA fragments have produced resolved CE peaks. Our results demonstrate that analyses of DNA constituents can be performed under a wide range of conditions by the appropriate tuning of the coating and filler within the capillary.

Anahtar Kelimeler:

Capillary electrophoresis, DNA, Polyethyleneimine

Polyethyleneimine-Coated Capillaries for the Separation of DNA by Capillary Electrophoresis

A new capillary electrophoresis of DNA fragments has been achieved with the cooperative effects of the electrophoretic and electroosmotic mobilities realized via the polyethyleneimine (PEI) coating of the capillary walls. As a result, seven of the 11 different-sized DNA fragments have produced resolved CE peaks. Our results demonstrate that analyses of DNA constituents can be performed under a wide range of conditions by the appropriate tuning of the coating and filler within the capillary.

Keywords:

Capillary electrophoresis, DNA, Polyethyleneimine,

Turkish Journal of Chemistry-Cover

ISSN: 1300-0527
Yayın Aralığı: 6
Yayıncı: TÜBİTAK

Arşiv

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