M. Said NUTKU, F. Bedia ERİM BERKER

2712

Polyethyleneimine-Coated Capillaries for the Separation of DNA by Capillary Electrophoresis

A new capillary electrophoresis of DNA fragments has been achieved with the cooperative effects of the electrophoretic and electroosmotic mobilities realized via the polyethyleneimine (PEI) coating of the capillary walls. As a result, seven of the 11 different-sized DNA fragments have produced resolved CE peaks. Our results demonstrate that analyses of DNA constituents can be performed under a wide range of conditions by the appropriate tuning of the coating and filler within the capillary.
Anahtar Kelimeler:

Capillary electrophoresis, DNA, Polyethyleneimine

Polyethyleneimine-Coated Capillaries for the Separation of DNA by Capillary Electrophoresis

A new capillary electrophoresis of DNA fragments has been achieved with the cooperative effects of the electrophoretic and electroosmotic mobilities realized via the polyethyleneimine (PEI) coating of the capillary walls. As a result, seven of the 11 different-sized DNA fragments have produced resolved CE peaks. Our results demonstrate that analyses of DNA constituents can be performed under a wide range of conditions by the appropriate tuning of the coating and filler within the capillary.
Keywords:

Capillary electrophoresis, DNA, Polyethyleneimine,

PDF
Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Differential Pulse Polarographic Determination of Enalapril Maleate

Fikriye ELMALI, Güzin ALPDOĞAN

A Novel Silver Recovery Method from Waste Photographic Films with NaOH Stripping

Nuri NAKİBOĞLU

Determination of $H_2O_2$ content of various water samples using a chemiluminescence technique

Elmas GÖK, Serdar ATEŞ, Tuğba TÜĞSÜZ

Photochemical Reactions of a-Terpinene and Acenaphthene under Concentrated Sunlight

Nesibe AVCIBAŞI and Sıddık İÇLİ

Thermal Decomposition Kinetics of Anilino-p-chlorophenylglyoxime Complexes of Cobalt (II), Nickel (II) and Copper (II)

Hakan ARSLAN, Nevzat KÜLCÜ

Search for a Nonelectrocyclic Cyclization of Nitrosostyrene: Rearrangements of Michael Adducts from DMAD and a-Dialkylamino Oximes

Necdet COŞKUN, Nevin ARIKAN

Determination of H2O2 Content of Various Water Samples Using a Chemiluminescence Technique

Tuğba TÜĞSÜZ, Elmas GÖK, Serdar ATEŞ

Polyethyleneimine-Coated Capillaries for the Separation of DNA by Capillary Electrophoresis

M. Said NUTKU, F. Bedia ERİM BERKER

Search for a nonelectrocyclic cyclization of nitrosostyrene: Rearrangements of Michael adducts from DMAD and $alpha$-dialkylamino oximes

Necdet COŞKUN, Nevin ARIKAN

Photochemical reactions of $alpha$-terpinene and acenaphthene under concentrated sunlight

Nesibe AVCIBAŞI, Andrew GILBERT, Sıddık İÇLİ