Ashutosh MUKHERJEE, Biswanath SIKDAR, Biplab GHOSH, Anuradha BANERJEE, Enakshi GHOSH, Moimee BHATTACHARYA, Satyesh Chandra ROY

RAPD and ISSR analysis of some economically important species, varieties, and cultivars of the genus Allium (Alliaceae)

RAPD and ISSR markers have been used to reveal the genetic diversity and phylogenetic analysis of some economically important species of Allium. Two plants related to Allium were also included for better understanding of the phylogeny. ISSR showed more polymorphism within A. cepa and A. sativum, while RAPD showed more polymorphism within A. porrum. Overall, RAPD revealed more intraspecific diversity than ISSR, while ISSR showed more interspecific diversity than RAPD. This showed the usefulness of using both markers for the study of Allium. UPGMA-based dendrograms showed a close relationship between Allium sativum and Allium porrum. A. porrum showed less genetic variability than A. cepa and A. sativum. Several unique bands were identified with RAPD and ISSR, which can be converted into cultivar-specific markers. Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. This study showed that rapid and cost-effective markers like RAPD and ISSR coupled with appropriate statistical tools can be successfully applied to study phylogenetic relationships at the interspecific and intraspecific level in Allium. These markers proved to be suitable for both phylogenetic studies among different species as well as for characterisation of different cultivars of Allium.

Anahtar Kelimeler:

Key words: Alliaceae, AMOVA, discriminating power, molecular characterisation, polymorphism

RAPD and ISSR analysis of some economically important species, varieties, and cultivars of the genus Allium (Alliaceae)

Keywords:

AMOVA, discriminating power, molecular characterisation, polymorphism, Alliaceae,

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Allium and related plant species also supports the traditional taxonomic treatments of these species. Further analysis together with data from other methods can be used to make a more accurate reconstruction of the genus Allium. Additionally, the large number of unique bands obtained in the present study signifies the power of RAPD and ISSR markers in fingerprinting and diversity analysis, especially at the lower taxonomic level. However, owing to the large number of accessions available for each of these species and varieties of Allium, it is important to screen the specificity of these bands over a large sample size before affirming their diagnostic capacity. Acknowledgements This work was financially supported by the Council of Scientific and Industrial Research of the government of India. We are grateful to Dr. Umesh Thapa, Bidhan Chandra Krishi Viswa Vidyalaya, West Bengal, India, for the identification of plant materials.