Zeba KHAN, Mohammad Yunus Khalil ANSARI, Honey GUPTA, Sana CHAUDHARY

Dynamics of 2,4-D in generation of cytomorphological variants in an important anticancerous and antihepatotoxic herb – Cichorium intybus L.

The genotoxic effect of 2,4-D was investigated in chicory (2n = 18). At 100 ppm concentration 4 variants differing in morphological traits were isolated and subjected to chromosomal analysis. Morphological variations in shape, height, and yield parameters were observed. Different cytological anomalies such as univalents, multivalents, bridges, laggards, chromosome stickiness, and polyads occurred in all 4 isolates. However, the frequency of these chromosomal irregularities decreased at anaphase, exhibiting recovery at later stages. Thus the above concentration of growth hormone 2,4-D can be effectively incorporated for raising viable mutants in this medicinally useful herb.

Anahtar Kelimeler:

Key words: 2, 4-D, Cichorium intybus

Dynamics of 2,4-D in generation of cytomorphological variants in an important anticancerous and antihepatotoxic herb – Cichorium intybus L.

Keywords:

Key words: 2, 4-D, Cichorium intybus,

PDF

___

Acharya SN, Thomas JE & Basu SK (2007). Improvement in the medicinal and nutritional properties of fenugreek (Trigonella foenum-graecum). In: Acharya SN, Thomas JE (eds) Advances in medicinal plant research, Research Signpost, Trivandrum, Kerela, India.
Akgun I & Tosun M (2004).Agricultural and cytological characteristics of M1 perennial rye (Secale montanum Guss.) as effected by the application of different doses of gamma rays. Pak J Biol Sci 7: 827-833.
Alizadeh H, Naghavi MR, Omidi M & Saatian B (2004). Effect of plant growth regulators on direct shoot regeneration of wheat
(Triticum aestivum). New direction for a diverse planet: Proceedings of the 4th International Crop Science Congress Brisbane, Australia. Amer SM & Ali EM (1974). Cytological effects of pesticides. V. Effects of some herbicides on Vicia faba. Cytologia 39: 633-643.
Audus LJ (1961). Growth regulators, “Metabolism and mode of action” Encyclopaedia of plant physiology. 14: 1055-1083
Springer Verlag Berlin. Gaulden ME (1987). Hypothesis: some mutagens directly alter specific chromosomal protein (DNA topoisomerase II and peripheral proteins) to produce chromosome stickiness which causes chromosome aberrations. Mutagenesis 2: 357-365
Grant WF (1978). Chromosome aberrations in plants as a monitoring system. Environ Health Perspect 27: 37-43.
Hartwell JL (1967-1971). Plants used against cancer. A survey. Lloydia 34. Telophase Control/ Variants of PMCs Abnormal PMCs Univalents Multivalents ChiasmaPrecocious Stray Chiasma Laggards Bridges Stickiness Polyads Bridges freq. separation chrom. freq. Control 215 _ _ 12 _ _ 32 – – – – – – Variant 1 35 80 43 60 15 02 25 93 70 90 45 11 Variant 2 91 73 84 73 73 23 65 81 82 36 91 80 Variant 3 43 19 38 19 31 48 07 75 75 31 87 91 Variant 4 86 04 03 47 04 16 73 30 17 86 43 39