Mariyana GEORGIEVA, İvelina NIKOLOVA, Georgi BONCHEV, Zornitsa KATEROVA, Dessislava TODOROVA

A comparative analysis of membrane intactness and genome integrity in pea,barley, and wheat in response to UVC irradiation

The maintenance of plant genome integrity plays a critical function in the processes of DNA replication, transcription, and repair. Short-wave UV radiation (UVC) is among the most harmful agents known to affect genome stability and to induce DNA damage, including double-strand breaks (DSBs). Most previous studies in plants addressed the effects of UVC radiation at the physiological level; however, little research effort has been put into genome sensitivity across different plant species. Here, we made use of the trypan blue exclusion test and neutral comet assay to assess nuclear membrane and genome integrity in response to UVC radiation in monocot and dicot plants. We found that UVC radiation substantially affects nuclear membranes and the level of DSBs in a dose-responsive manner. Furthermore, differential sensitivity across plant species was observed, with monocot plants being less vulnerable to DSBs. This allows us to speculate that plant species with larger genomes may better tolerate UVC radiation.

Anahtar Kelimeler:

Ultraviolet radiation, genome integrity, trypan blue exclusion test, neutral comet assay, DNA double-strand breaks

A comparative analysis of membrane intactness and genome integrity in pea,barley, and wheat in response to UVC irradiation

Keywords:

Ultraviolet radiation, genome integrity, trypan blue exclusion test, neutral comet assay, DNA double-strand breaks,

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Armalyte J, Žukas K (2002). Evaluation of UVC-induced DNA damage by SCGE assay and its repair in barley. Biologija 3: 57–59.
Bogdanov KV, Chukhlovin AB, Zaritskey AY, Frolova OI, Afanasiev BV (1997). Ultraviolet irradiation induces multiple DNA double-strand breaks and apoptosis in normal granulocytes and chronic myeloid leukaemia blasts. Br J Haematol 98: 869– 872.
Cotelle S, Férard JF (1999). Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 34: 246–255.
Frohnmeyer H, Staiger D (2003). Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133: 1420–1428.
Georgieva M, Stoilov L (2008). Assessment of DNA strand breaks induced by bleomycin in barley by the comet assay. Environ Mol Mutagen 49: 381–387.
Gichner T, Plewa MJ (1998). Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutat Res 401: 143–152.
Heddle JA, Athanasiou K (1975). Mutation rate, genome size and their relation to the rec concept. Nature 258: 359–361.
Kakani VG, Reddy KR, Zhao D, Sailaja K (2003). Field crop responses to ultraviolet-B radiation: a review. Agr Forest Meteorol 120: 191–218.
Kulms D, Schwarz T (2002). Molecular mechanisms involved in UV-induced apoptotic cell death. Skin Pharmacol Appl Skin Physiol 15: 342–347.
Kunz BA, Cahill DM, Mohr PG, Osmond MJ, Vonarx EJ (2006). Plant responses to UV radiation and links to pathogen resistance. Int Rev Cytol 255: 1–40.
Leitch IJ, Beaulieu JM, Chase MW, Leitch AR, Fay MF (2010). Genome size dynamics and evolution in monocots. Journal of Botany 2010: 862516.
Lovell DP, Thomas G, Dubow R (1999). Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies. Teratog Carcinog Mutag 19: 109–119.
Ma W, Panduri V, Sterling JF, Van Houten B, Gordenin DA, Resnick MA (2009). The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1. Mol Cell Biol 29: 1212–1221.
Macas J, Neumann P, Navratilova A (2007). Repetitive DNA in the pea (Pisum sativum L.) genome: comprehensive characterization using 454 sequencing and comparison to soybean and Medicago truncatula. BMC Genomics 8: 427.
Mahdavian L, Ghorbanli M, Kalantari KM (2008). The effects of ultraviolet radiation on some antioxidant compounds and enzymes in Capsicum annuum. Turk J Bot 32: 129–134.
Mou Z, He Y, Dai Y, Liu X, Li J (2000). Deficiency in fatty acid synthase leads to premature cell death and dramatic alterations in plant morphology. Plant Cell 12: 405–418.
Mpoloka SW (2008). Effects of prolonged UV-B exposure in plants. Afr J Biotechnol 7: 4874–4883.
Myllyperkiö MH, Koski TR, Vilpo LM, Vilpo JA (2000). Kinetics of excision repair of UV-induced DNA damage, measured using the comet assay. Mutat Res 448: 1–9.
Nikolova I, Georgieva M, Stoilov L, Katerova Z, Todorova D (2013). Optimization of Neutral Comet Assay for studying DNA double-strand breaks in pea and wheat. J BioSci Biotech 2: 151–157.
Qüesta JI, Walbot V, Casati P (2010). Mutator transposon activation after UV-B involves chromatin remodeling. Epigenetics 5: 352–363.
Salse J, Bolot S, T’hroude M, Jouffe V, Piegu B, Quraishi UM, Calcagno T, Cooke R, Delseny M, Feuillet C (2008). Identification and characterization of shared duplications between rice and wheat provide new insight into grass genome evolution. Plant Cell 20: 11–24.
Stapleton AE (1992). Ultraviolet radiation and plants: burning questions. Plant Cell 4: 1353–1358.
Stoilov L, Georgieva M, Manova V, Liu L, Gecheff K (2013). Karyotype reconstruction modulates the sensitivity of barley genome to radiation-induced DNA and chromosomal damage. Mutagenesis 28: 153–160.
Tashiro S (2000). Rad51 accumulation at sites of DNA damage and in postreplicative chromatin. J Cell Biol 150: 283–292.
Todorova D, Katerova Z, Shopova E, Nikolova A, Georgieva N, Sergiev I, Mapelli S (2013). Polyamine spermine protects young pea plants against ultraviolet-C radiation. Biotechnol Biotechnol Eq 27: 3798–3802.
Wong X, Luperchio TR, Reddy KL (2014). NET gains and losses: the role of changing nuclear envelope proteomes in genome regulation. Curr Opin Cell Biol 28: 105–120.
Zemp FJ, Sidler C, Kovalchuk I (2012). Increase in recombination rate in Arabidopsis thaliana plants sharing gaseous environment with X-ray and UVC-irradiated plants depends on production of radicals. Plant Signal Behav 7: 782–787.