Transgenic Nicotiana tabacum cultivar Samsun plants carrying the wild sugar beet Hs1pro1 gene have resistance to root-knot nematodes
Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1pro1, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1pro1 has a broad target pathogen range. In this study, we transformed Nicotiana tabacum cultivar Samsun plants with the Hs1pro1 gene and assayed transgenic plants for nematode resistance. Our analysis suggests that compared to wild-type plants, Hs1pro1-carrying transgenic plants are more resistant to 2 different root-knot nematode species, Meloidogyne incognita and Meloidogyne javanica. The results in this study support previous findings of the broad host range of Hs1pro1 and are promising in terms of the genetic engineering of economically important susceptible crop plants such as potato and tomato against nematode and secondary damage from other soil-pathogen damage.
Transgenic Nicotiana tabacum cultivar Samsun plants carrying the wild sugar beet Hs1pro1 gene have resistance to root-knot nematodes
Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1pro1, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1pro1 has a broad target pathogen range. In this study, we transformed Nicotiana tabacum cultivar Samsun plants with the Hs1pro1 gene and assayed transgenic plants for nematode resistance. Our analysis suggests that compared to wild-type plants, Hs1pro1-carrying transgenic plants are more resistant to 2 different root-knot nematode species, Meloidogyne incognita and Meloidogyne javanica. The results in this study support previous findings of the broad host range of Hs1pro1 and are promising in terms of the genetic engineering of economically important susceptible crop plants such as potato and tomato against nematode and secondary damage from other soil-pathogen damage.
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- Atkinson HJ, Urwin EP, Hansen E, McPherson MJ (1995). Designs for engineered resistance to root-parasitic nematodes. Trends Biotechnol 13: 369–374.
- Ayoub SM (1980). Plant Nematology: An Agricultural Training Aid. Sacramento, CA, USA: NemaAid Publications.
- Baena-González E, Sheen J (2008). Convergent energy and stress signaling. Trends Plant Sci 9: 474–482.
- Baker B, Zambryski P, Staskawicz B, Dinesh-Kumar SP (1997). Signaling in plant-microbe interactions. Science 276: 726–733.
- Barker KR (1985). Nematode extraction and bioassays. In: Barker KR, Carter CC, Sasser JN, editors. An Advanced Treatise on Meloidogyne, Volume 2. Methodology. Raleigh, NC, USA: North Carolina State University Press, pp. 19–35. C ai D, Kleine M, Kifle S, Harloff HJ, Sandal NN, Marcker KA, KleinLankhorst RM, Salentjin EMJ, Lange W, Stiekema WJ et al. (1997). Positional cloning of a gene for nematode resistance in sugar beet. Science 275: 832–834.
- De Wit PJGM (1997). Pathogen avirulence and plant resistance: a key role for recognition. Trends Plant Sci 2: 452–458.
- Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
- Fenoll C, Grundler FMW, Ohl SA (1997). Cellular and Molecular Aspects of Plant-Nematode Interactions. Dordrecht, the Netherlands: Kluwer Academic Publishers.
- G ilardoni PA, Schuck S, Jüngling R, Rotter B, Baldwin IT, Bonaventure G (2010). SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses. BMC Plant Biol 10: 66.
- Hammond-Kosack KE, Jones JDG (1997). Plant disease resistance genes. Annu Rev Plant Physio 48: 573–607.
- Horsch RB, Hry JE, Hoffman NL, Rogers RT, Farley RT (1985). A simple and general method for transferring genes into plants. Science 227: 1227–1232.
- Jenkins WR, Taylor DP (1967). Plant Nematology. New York, NY, USA: Reinhold Publishing Corporation
- Khan MR, Mohiddin FA, Ejaz MN, Khan MM (2012). Management of root-knot disease in eggplant through the application of biocontrol fungi and dry neem leaves. Turk J Biol 36: 161–169.
- Kramer C, DiMaio J, Carswell GK, Shillito RD (1993). Selection of transformed protoplast-derived Zea mays colonies with phosphinothricin and a novel assay using the pH indicator chlorophenol red. Planta 190: 454–458.
- Linn JJ (1995). Electrotransformation of Agrobacterium. Method Mol Cell Biol 47: 171–178.
- Merseraau M, Pazour GJ, Das A (1990). Efficient transformation of Agrobacterium tumefaciens by electroporation. Gene 90: 149– 1
- Muller J (1999). The economic importance of Heterodera schachtii in Europe. Helminthologia 36: 205–213.
- Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plantarum 15: 473–497.
- Ohl SA, van der Lee FM, Sijmons PC (1997). Anti-feeding structure approaches to nematode resistance. Dev Plant Pathol 10: 250– 2
- Öktem HA, Özkan F, Özalp VC, Yücel M (1994). Agrobacterium mediated gene transfer in tobacco. Turk J Bot 18: 397–405.
- Sasser JN (1990). Plant-Parasitic Nematodes: The Farmer’s Hidden Enemy. Raleigh, NC, USA: North Carolina State University Press.
- Schuck S, Camehl I, Gilardoni PA, Oelmueller R, Baldwin IT, Bonaventure G (2012). HSPRO controls early Nicotiana attenuata seedling growth during interaction with the fungus Piriformospora indica. Plant Physiol 160: 929–943.
- Sijmons PC (1993). Plant-nematode interactions. Plant Mol Biol 23: 917–931.
- Strauch E (1988). Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63: 65–74.
- Tariq M, Dawar S, Mehdi FS, Zaki MJ (2007). Use of Avicennia marina (Forsk.) Vierh in the control of root knot nematode Meloidogyne javanica (Treub) Chitwood on okra and mash bean. Turk J Biol 31: 225–230.
- Thompson CJ (1987). Characterization of the herbicide resistance gene bar from Streptomyces hygroscopicus. EMBO J 6: 2519– 25
- Vos P, Simons G, Jesse T, Wijbrandi J, Heinen L, Hogers R, Frijters A, Groenendijk J, Diergaarde P, Reijans M et al. (1998). The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids. Nat Biotechnol 16: 1365–1369.