Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks

Öz A novel coronavirus (SARS-CoV-2, formerly known as nCoV-2019) that causes an acute respiratory disease has emerged in Wuhan, China and spread globally in early 2020. On January the 30th, the World Health Organization (WHO) declared spread of this virus as an epidemic and a public health emergency. With its highly contagious characteristic and long incubation time, confinement of SARS-CoV-2 requires drastic lock-down measures to be taken and therefore early diagnosis is crucial. We analysed transcriptome of SARS-CoV-2 infected human lung epithelial cells, compared it with mock-infected cells, used network-based reporter metabolite approach and integrated the transcriptome data with protein-protein interaction network to elucidate the early cellular response. Significantly affected metabolites have the potential to be used in diagnostics while pathways of protein clusters have the potential to be used as targets for supportive or novel therapeutic approaches. Our results are in accordance with the literature on response of IL6 family of cytokines and their importance, in addition, we find that matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9) with keratan sulfate synthesis pathway may play a key role in the infection. We hypothesize that MMP9 inhibitors have potential to prevent "cytokine storm" in severely affected patients.

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Kaynak Göster

Bibtex @araştırma makalesi { tbtkbiology943536, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {}, publisher = {TÜBİTAK}, year = {2020}, volume = {44}, pages = {168 - 177}, doi = {}, title = {Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks}, key = {cite}, author = {Karakurt, Hamza Umut and Pir, Pinar} }
APA Karakurt, H , Pir, P . (2020). Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks . Turkish Journal of Biology , Special Issue on COVID-19 , 168-177 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkbiology/issue/62502/943536
MLA Karakurt, H , Pir, P . "Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks" . Turkish Journal of Biology 44 (2020 ): 168-177 <https://dergipark.org.tr/tr/pub/tbtkbiology/issue/62502/943536>
Chicago Karakurt, H , Pir, P . "Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks". Turkish Journal of Biology 44 (2020 ): 168-177
RIS TY - JOUR T1 - Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks AU - Hamza Umut Karakurt , Pinar Pir Y1 - 2020 PY - 2020 N1 - DO - T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 168 EP - 177 VL - 44 IS - SI-1 SN - 1300-0152-1303-6092 M3 - UR - Y2 - 2020 ER -
EndNote %0 Turkish Journal of Biology Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks %A Hamza Umut Karakurt , Pinar Pir %T Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks %D 2020 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 44 %N SI-1 %R %U
ISNAD Karakurt, Hamza Umut , Pir, Pinar . "Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks". Turkish Journal of Biology 44 / SI-1 (Haziran 2020): 168-177 .
AMA Karakurt H , Pir P . Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks. Turkish Journal of Biology. 2020; 44(SI-1): 168-177.
Vancouver Karakurt H , Pir P . Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks. Turkish Journal of Biology. 2020; 44(SI-1): 168-177.
IEEE H. Karakurt ve P. Pir , "Integration of transcriptomic profile of SARS-CoV-2 infected normal human bronchial epi-thelial cells with metabolic and protein-protein interaction networks", Turkish Journal of Biology, c. 44, sayı. SI-1, ss. 168-177, Haz. 2020

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