KATARZYNA MICHALEK, EWA CZERNIAWSKA-PIATKOWSKA, MARTA GRABOWSKA, MARIA LASZCZYNSKA

Immunohistochemical identification of aquaporin 2 in the kidneys of wild boars (Sus scrofa)

Abstract: Mammalian aquaporins (AQPs) are a family of at least 13 small, integral membrane proteins expressed in various fluid-transporting epithelial cells. Aquaporin 2 (AQP2) is mainly expressed in the kidneys, where it plays an important role in the reabsorption of water from the renal tubular fluid to concentrate urine. Studies of AQP2 expression in the kidneys have been performed in humans, monkeys, sheep, dogs, rabbits, rats, mice, and cattle. We analyzed the expression of AQP2 in the kidneys of wild boars (Sus scrofa). AQP2 was localized in the principal cells of collecting ducts in medullary rays penetrating the renal cortex and in the collecting duct of the renal medulla. The presence of AQP2 was confirmed mainly in the apical plasma membrane but also in the intracellular components and basolateral membrane of the principal cells of the collecting ducts. Due to the key role of AQP2 in the regulation of water balance in mammals and the lack of studies in the literature concerning analysis of this protein in wild boars, this work completes the present state of knowledge by providing new information on immunolocalization and immunoexpression of aquaporin 2 in Sus scrofa kidneys.

Anahtar Kelimeler:

Aquaporin 2, wild boars, immunohistochemistry, kidney

Immunohistochemical identification of aquaporin 2 in the kidneys of wild boars (Sus scrofa)

Keywords:

Aquaporin 2, wild boars, immunohistochemistry, kidney,

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Agre P, Preston GM, Smith BL, Jung JS, Raina S, Moon C, Guggino WB, Nielsen S (1993). Aquaporin chip: the archetypal molecular water channel. Am J Physiol Renal Physiol 265: F463–F476.
Bauchet AL, Masson R, Guffroy M, Slaoui M (2011). Immunohistochemical identification of kidney nephron segments in the dog, rat, mouse, and cynomolgus monkey. Toxicol Pathol 39: 1115–1128.
Benga G (2009). Water channel proteins (later called aquaporins) and relatives: past, present, and future. IUMB Life 6: 112–133.
Boone M, Deen P (2008). Physiology and pathophysiology of the vasopressin- regulated renal water reabsorption. Eur J Physiol 456: 1005–1024.
Christensen BM, Zelenina M, Aperia A, Nielsen S (2000). Localization and regulation of PKA-phosphorylated AQP2 in response to V(2)-receptor agonist/antagonist treatment. Am J Physiol Renal Physiol 278: F29–42.
Drolet R (2012). Urinary system. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, editors. Disease of Swine. 10th ed. Oxford, UK: Wiley-Blackwell, pp. 263–283.
Eskild-Jedsen A, Paulsen LF, Wogensen FL, Olesen P, Pedersen L, Frøkiær J, Nyengaard JR (2007). AT1 receptor blockade prevents interstitial and glomerular apoptosis but not fibrosis in pigs with neonatal induced partial unilateral obstruction. Am J Physiol Renal Physiol 292: F1771–F1781.
Fenton R, Moeller HB, Hoffert DJ, Yu MJ, Nielsen S, Knepper M (2008). Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin. Proc Natl Acad Sci USA 105: 3134– 3139.
Fenton RA, Knepper MA (2007). Mouse models and the urinary concentrating mechanism in the new millennium. Physiol Rev 87: 1083–1112.
Floyd RV, Mason SL, Proudman CJ, German AJ, Marples D, Mobasheri A (2007). Expression and nephron segmentspecific distribution of major renal aquaporins (AQP1-4) in Equus caballus, the domestic horse. Am L Physiol Integr Comp Physiol 293: 492–503.
Fushimi K, Uchida S, Hara Y, Hirata Y, Marumo F, Sasaki S (1993). Cloning and expression of apical membrane water channel of rat kidney collecting tubule. Nature 361: 549–552.
Gomes G, Agasse A, Thiébaud P, Delrot S, Gerós H, Chaumont F (2009). Aquaporins are multifunctional water and solute transporters highly divergent in living organisms. Biochim Biophys Acta 1788: 1213–1228.
Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA (2008). Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 36: 24617–24627.
Hoffert JD, Nielsen J, Yu MJ, Pisitkun T, Schleicher SM, Nielsen S, Knepper MA (2007). Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691–700.
Holmes R (2012). The role of renal water channels in health and disease. Mol Aspects Med 33: 547–552.
Hub JS, Groot B (2008). Mechanism of selectivity in aquaporins and aquaglyceroporins. Proc Natl Acad Sci USA 105: 1198–1203.
Ketz HA (1960). Comparative studies on renal function in domestic animals. Arch Exp Vet Med 14: 411–419.
Kishore BK, Terrsi JM, Knepper MA (1996). Rat renal arcade segment expressed vasopressin-regulated water channel and vasopressin V2 receptor. J Clin Invest 97: 2733–2771.
Kortenoeven LA, Fenton RA (2014). Renal aquaporins and water balance disorders. Biochim Biophys Acta 1840: 1533–1549.
Loffing J, Loffing-Cueni D, Macher A, Hebert SC, Olson B, Knepper MA, Rossier BC, Kaissling B (2000). Localisation of epithelial sodium channel and aquaporin-2 in rabbit kidney cortex. Am J Physiol Renal Physiol 278: F530–F539.
Lou W, Zhanjun L, Huang Y, Han Y, Yao Ch, Duan X, Ouyang H, Li L (2014). Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells. Transgenic Res 23: 365–375.
Marples D, Knepper MA, Christensen EI, Nielsen S (1995). Redistribution of aquaporin-2 water channels induced by vasopressin in rat kidney inner medullary collecting duct. Am J Physiol Cell Physiol 269: 655–664.
Michałek K, Laszczyńska M, Ciechanowicz AK, Herosimczyk A, Rotter I, Oganowska M, Lepczyński A, Dratwa-Chałupnik A (2014). Immunohistochemical identification of aquaporin 2 in the kidneys of young beef cattle. Biotech & Histochem 89: 342–347.
Mobasheri A, Kendall BH, Maxwell JEJ, Sawran AV, German AJ, Marples D, Luck M, Royal MD (2011). Cellular localization of aquaporins along the secretory pathway of the lactating bovine mammary gland: an immunohistochemical study. Acta Histochem 113: 137–149.
Moeller HB, Fenton RA (2012). Cell biology of vasopressin-regulated aquaporin-2 trafficking. Eur J Physiol 464: 133–144.
Moeller HB, Knepper MA, Fenton RA (2009). Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int 3: 295–303.
Nielsen S, DiGiovani SR, Christensen EI, Knepper MA, Harris HW (1993). Cellular and subcellular immunolocalization of vasopressin regulated water channel in rat kidney. Proc Natl Acad Sci USA 90: 11663–11667.
Nielsen S, Frøklær J, Marples D, Kwon T, Agre P, Knepper M (2002). Aquaporins in the kidney. From molecules to medicine. Physiol Rev 82: 205–244.
Nielsen S, Kwon T, Frøkiær J, Knepper MA (2000). Key roles of renal aquaporins in water-balance and water-balance disorders. News Physiol Sci 15: 136–143.
Noda Y, Sasaki S (2005). Trafficking mechanism of water channel aquaporin-2. Biol Cell 97: 885–892.
Pilarczyk B, Hendzel D, Pilarczyk R, Tomza-Marciniak A, Błaszczyk B, Dąbrowska-Wieczorek M, Bąkowska M, Adamowicz E, Bujak T (2010). Liver and kidney concentrations of selenium in wild boars (Sus scrofa) from northwestern Poland. Eur J Wild Res 56: 797–802.
Rojek A, Füchtbauer E, Kwon T, Frøkiær J, Nielsen S (2006). Severe urinary concentrating defect in renal collecting duct-selective AQP2 conditional-knockout mice. Proc Natl Acad Sci USA 15: 6037–6042.
Ruckebusch Y, Phaneuf LP, Dunlop R (1991). The urinary collecting and voiding system. In: Ruckebusch Y, Phaneuf LP, Dunlop R, editors. Physiology of Small and Large Animals. 1st ed. Philadelphia, PA, USA: BC Decker, pp. 184–188 . Sasaki S (2012). Aquaporin 2: from its discovery to molecular structure and medical implications. Mol Aspects Med 33: 535–546.
Takata K, Matsuzaki T, Tajika Y, Ablimit A, Hasegawa T (2008). Localization and trafficking of aquaporin 2 in the kidney. Histochem Cell Biol 130: 197–209.
Wilson JLL, Miranda CA, Knepper MA (2013). Vasopressin and the regulation of aquaporin-2. Clin Exp Nephrol 6: 751–764.
Yamamoto T, Sasaki S, Fushimi K, Kawasaki K, Yaoita E, Oota K, Hirata Y, Marmuro F, Kihara I (1995). Localization and expression of a collecting duct water channel, aquaporin, in hydrated and dehydrated rats. Exp Nephrol 3: 193–201.