Cell-compatible PHB/silk fibroin composite nanofiber mat for tissue engineering applications

Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were prepared through the use of the electrospinning technique, which is a unique method for the production of biomaterials on the nanoscale intended for tissue engineering. PHB/SF scaffolds were prepared with different SF contents and a suitable electrospinning condition was chosen in terms of structure and fiber diameter. The average fiber diameter was 92 ± 2.6 nm at a flow rate of 0.4 mL/h, distance of 20 cm, polymer concentration of PHB/SF-0 of 7%:18% (w/w) or 1:1 (v/v), and voltage of 20 kV. Mechanical and crystalline properties of the PHB/SF scaffold were investigated. Adhesion and proliferation of L929 and HaCaT (mouse fibroblast and immortalized human keratinocyte cell lines) on PHB/SF-0 were examined

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Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL (2003). Silk-based biomaterials. Biomaterials 24: 401-416.
Alves EGL, Rezende CMF, Serakides R, Pereira MM, Rosado IR (2011). Orthopedic implant of a polyhydroxybutyrate (PHB) and hydroxyapatite composite in cats. J Feline Med Surg 13: 546-552.
Basal G, Tetik GD, Kurkcu G, Bayraktar O, Gurhan ID, Atabey A (2016). Olive leaf extract loaded silk fibroin/hyaluronic acid nanofiber webs for wound dressing applications. Dig J Nanomater Biostruct 11: 1113-1123.
Bhattacharjee P, Naskar D, Kim H, Maiti TK, Bhattacharya D, Kundu SC (2015). Non-mulberry silk fibroin grafted PCL nanofibrous scaffold: promising ECM for bone tissue engineering. Eur Polym J 71: 490-509.
Cai Q, Wan Y, Bei Y, Wang S (2003). Synthesis and characterization of biodegradable polylactide-grafted dextran and its application as compatilizer. Biomaterials 24: 3555-3562.
Cai Q, Yang J, Bei J, Wang S (2002). A novel porous cells scaffold made of polylactide-dextran blend by combining phaseseparation and particle-leaching techniques. Biomaterials 23: 4483-4492.
Cai ZX, Mo XM, Zhang KH, Fan LP, Yin AL, He CI, Wang HS (2010). Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications. Int J Mol Sci 11: 3529-3539. Chen G, Ushida T, Tateishi T (2002). Scaffold design for tissue engineering. Macromol Biosci 2: 67-77.
Daranarong D, Chan RTH, Wanandy NS, Molloy R, Punyodom W, Foster LJR (2014). Electrospun polyhydroxybutyrate and poly(L-lactide-co-ε-caprolactone) composites as nanofibrous scaffolds. Biomed Res Int 2014: 12.
da Silva MA, Oliveira RN, Mendonça RH, Lourenço TG, Colombo AP, Tanaka MN, Tude EM, da Costa MF, Thiré RM (2016). Evaluation of metronidazole-loaded poly(3-hydroxybutyrate) membranes to potential application in periodontitis treatment. J Biomed Mater Res B Appl Biomater 104: 106-115.
Drumheller P, Hubbell J (2000). The Biomedical Engineering Handbook. CRC Press.
Fedorov MB, Vikhoreva GA, Kildeeva NR, Mokhova ON, Bonartseva GA, Galbraikh LS (2007). Antimicrobial activity of coresheath surgical sutures modified with poly-3-hydroxybutyrate. Prikl Biokhim Mikrobiol 43: 685-690 (in Russian with abstract in English).
Feng L, Li S, Li H, Zhai J, Song Y, Jiang L, Zhu D (2002). Superhydrophobic surface of aligned polyacrylonitrile nanofibers. Angew Chem Int Ed Engl 41: 1221-1223.
Ha SW, Tonelli AE, Hudson SM (2005). Structural studies of Bombyx mori silk fibroin during regeneration from solutions and wet fiber spinning. Biomacromolecules 6: 1722-1731.
Hubbell JA (1995). Biomaterials in tissue engineering. Biotechnology 13: 565-576.
Ikejima T, Inoue Y (2000). Crystallization behavior and environmental biodegradability of the blend films of poly(3- hydroxybutyric acid) with chitin and chitosan. Carbohydr Polym 41: 351-356.
Inpanya P, Faikrua A, Ounaroon A, Sittichokechaiwut A, Viyoch J (2012). Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats. Biomed Mater 7: 035008.
Jin HJ, Chen J, Karageorgiou V, Altman GH, Kaplan DL (2004). Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 25: 1039-1047.
Karahaliloğlu Z, Demirbilek M, Şam M, Erol-Demirbilek M, Sağlam N, Denkbaş EB (2013). Plasma polymerization-modified bacterial polyhydroxybutyrate nanofibrillar scaffolds. J Apply Polym Sci 128: 1904-1912.
Kim H, Che L, Ha Y, Ryu W (2014). Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles. Mater Sci Eng C Mater Biol Appl 40: 324-335.
Kim UJ, Park C, Li C, Jin HJ, Valluzzi R, Kaplan DL (2004). Structure and properties of silk hydrogels. Biomacromolecules 5: 786- 792.
Kim UJ, Park J, Kim HJ, Wada M, Kaplan DL (2005). Threedimensional aqueous-derived biomaterial scaffolds from silk fibroin. Biomaterials 26: 2775-2785.
Ko JS, Yoon K, Ki CS, Kim HJ, Bae DG, Lee KH, Park YH, Um IC (2013). Effect of degumming condition on the solution properties and electrospinnablity of regenerated silk solution. Int J Biol Macromol 55: 161-168.
Kundu J, Chung YI, Kim YH, Tae G, Kundu SC (2010). Silk fibroin nanoparticles for cellular uptake and control release. Int J Pharm 388: 242-250.
Lei C, Zhu H, Li J, Li J, Feng X, Chen J (2014). Preparation and characterization of polyhydroxybutyrate-cohydroxyvalerate/ Silk fibroin nanofibrous scaffolds for skin tissue engineering. Polym Eng Sci 55: 907-916.
Marelli B, Alessandrino A, Farè S, Freddi G, Mantovani D, Tanzi MC (2010). Compliant electrospun silk fibroin tubes for small vessel bypass grafting. Acta Biomater 6: 4019-4026.
Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004). Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25: 1289-1297.
Neimark A, Kornev K, Ravikovitch P, Ruetsch S (2003). Wetting of nanofibers. Poly Prepr 44: 160.
Rennukka M, Sipaut CS, Amirul AA (2014). Synthesis of poly(3- hydroxybutyrate-co-4-hydroxybutyrate)/chitosan/silver nanocomposite material with enhanced antimicrobial activity. Biotechnol Prog 30: 1469-1479.
Rosso F, Marino G, Giordano A, Barbarisi M, Parmeggiani D, Barbarisi A (2005). Smart materials as scaffolds for tissue engineering. J Cell Physiol 203: 465-470.
Silva GA, Coutinho OP, Ducheyne P, Reis RL (2007). Materials in particulate form for tissue engineering. 2. Applications in bone. J Tissue Eng Regen Med 1: 97-109.
Sukigara S, Gandhi M, Ayutsede J, Micklus M, Ko F (2003). Regeneration of Bombyx mori silk by electrospinning. Part 1. Processing parameters and geometric properties. Polymer 44: 5721-5727.
Sun M, Zhou P, Pan LF, Liu S, Yang HX (2009). Enhanced cell affinity of the silk fibroin- modified PHBHHx material. J Mater Sci Mater Med 20: 1743-1751.
Suwantong O, Waleetorncheepsawat S, Sanchavanakit N, Pavasant P, Cheepsunthorn P, Bunaprasert T, Supaphol P (2007). In vitro biocompatibility of electrospun poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fiber mats. Int J Biol Macromol 40: 217-223.
Vepari C, Kaplan DL (2007). Silk as a biomaterial. Prog Polym Sci 32: 991-1007.
Wang SD, Zhang YZ, Wang HW, Yin G, Dong Z (2009). Fabrication and properties of the electrospun polylactide/silk fibroin gelatin composite tubular scaffold. Biomacromolecules 10: 2240-2244.
Wang Y, Kim HJ, Vunjak-Novakovic G, Kaplan DL (2006). Stem cellbased tissue engineering with silk biomaterials. Biomaterials 27: 6064-6082.
Yang HX, Sun M, Zhang Y, Zhou P (2011). Degradable PHBHHx modified by the silk fibroin for the applications of cardiovascular tissue engineering. ISRN Material Sciences 2011: 389872.
Zhang H, Li L, Dai F, Zhang H, Bing N, Zhou W, Yang X, Wu Y (2012). Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery. J Transl Med 10: 117.
Zhang J, Mo X (2013). Current research on electrospinning of silk fibroin and its blends with natural and synthetic biodegradable polymers. Front Mater Sci 7: 129-142.
Zhong XH, Kim KS, Fang DF, Ran SF, Hsiao BS, Chu B (2002). Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer 43: 4403.