Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia

Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and a-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential oils and methanolic extracts of the plants were tested for antimicrobial activity using the disk-diffusion method against 8 bacteria and 1 fungus. Staphylococcus aureus was the most sensitive bacteria to all of the essential oils. A. santonicum and A. scoparia were the most active plants against Candida albicans, with 35-mm and 40-mm diameter zones, respectively. Antioxidant capacities of the plants were also tested. The radical scavenging activity of A. scoparia extract (48.51%) and essential oil (80.08%), the Trolox equivalent antioxidant capacity of A. campestris extract (10.76 ± 0.47), and the a-tocopherol equivalent of A. absinthium extract (5.87 ± 0.17) were the highest results.

Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia

Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and a-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential oils and methanolic extracts of the plants were tested for antimicrobial activity using the disk-diffusion method against 8 bacteria and 1 fungus. Staphylococcus aureus was the most sensitive bacteria to all of the essential oils. A. santonicum and A. scoparia were the most active plants against Candida albicans, with 35-mm and 40-mm diameter zones, respectively. Antioxidant capacities of the plants were also tested. The radical scavenging activity of A. scoparia extract (48.51%) and essential oil (80.08%), the Trolox equivalent antioxidant capacity of A. campestris extract (10.76 ± 0.47), and the a-tocopherol equivalent of A. absinthium extract (5.87 ± 0.17) were the highest results.

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  • Wr ight CW. Artemisia. Taylor & Francis. New York; 2002. 2. Oberprieler C. Temporal and spatial diversifi cation of Circum- Mediterranean Compositae-Antemideae. Taxon 54: 951-966, 2005. 3. F urlenmeir MM. Wunderwelt der Heilpfl anzen. RVG. Zurich; 1983.
  • Ho se S. Der Wermut - Artemisia absinthium L. Zeitschrift Phytother 23: 187-194, 2002. 5. Ba ytop T. Türkiye’de Bitkiler ile Tedavi: Geçmişte ve Bugün. Nobel Tıp Kitabevi. İstanbul; 1999.
  • Uzun E, Sariyar G, Adsersen A et al. Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected plants. J Ethnopharmacol 95: 287-296, 2004.
  • Tümen G, Sekendiz O. Plants used in traditional medicine in Balıkesir and central villages. Uludağ University Research Funding, Project No: 86/12, Balıkesir; 1989: pp. 19-22. 8. Akalın E. Wil d Plants Used in Tekirdağ Region as Medicine and Food, MSc, İstanbul University Institute of Health Sciences, 1993. 9. ESCOP.
  • Absinthii herba. In: ESCOP (European Scientifi c
  • Cooperative on Phytotherapy) Monographs on the Medicinal
  • Uses of Plant Drugs. Fascicule I; 1997: pp. 1-5.
  • Korkmaz H, Gürdal A. Eff ect of Artemisia santonicum L. on blood glucose in normal and alloxan-induced diabetic rabbits. Phytother Res 16: 675-676, 2002.
  • Kalemba D, Kusewic z D, Swiader K. Antimicrobial properties of the essential oil of Artemisia asiatica Nakai. Phytother Res 16: 288-291, 2002.
  • Tan RX, Zheng WF, Tang HQ. Biologically active substances from the genus Artemisia. Planta Med. 64: 295-302, 1998.
  • Davis PH. Artemisia. In: Flora of Turkey and the East Aegean Islands, Vol. 5. Edinburgh University Press; 1975: pp. 311-323.
  • Davis PH, Mill RR, Tan KK. Artemisia. In: Flora of Turkey and the East Aegean Islands, Vol. 10. Edinburgh University Press; 1988: pp. 163-164.
  • Khalilov LM, Param anov EA, Khalilova AZ et al. Identifi cation and biological activity of volatile organic compounds emitted by plants and insects. IV. Composition of vapor isolated from certain species of Artemisia plants. Chem Nat Comp 37: 339- 342, 2001.
  • Jerkoviç I, Masteliç J, Milos M et al. Chemical variability of Artemisia vulgaris L. essential oil originated from the Mediterranean area of France and Croatia. Phytotherapy Res 18: 436-440, 2003.
  • Akrout A, Chemli R, Ch erif I et al. Analysis of the essential oil of Artemisia campestris L. Flavour Fragr J 16: 337-339, 2001.
  • Akrout A, Chemli R, Si mmonds M et al. Seasonal variation of the essential oil of Artemisia campestris L. J Ess Oil Res 15: 333- 336, 2003.
  • Haider F, Dwivedi PD, Nagvi AA et al. Essential oil composition of Artemisia vulgaris harvested at diff erent growth periods under indo-gangetic plain conditions. J Ess Oil Res 15: 376- 378, 2003.
  • Masotti V, Juteu F, Bessiere JM et al. Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. J Agric Food Chem 51: 7115-7121, 2003.
  • Basher KHC, Ozek T, De mirchakmak B et al. Essential oils of some Artemisia species from Central Asia. Khim Priv Soedin 3: 383-385, 1997.
  • Juteu F, Jerkovic I, M asotti V et al. Composition and antimicrobial activity of the essential oil of Artemisia absinthium from Croatia and France. Planta Med 69: 158-161, 2003.
  • Juteu F, Masotti V, Be ssiere JM et al. Compositional characteristics of the essential oil of Artemisia campestris var. glutinosa. Biochem Syst Ecol 30: 1065-1070, 2002.
  • Kordali S, Cakir A, Mavi A et al. Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. J. Agric Food Chem 53: 1408-1416, 2005.
  • Demirci B, Demirci F, Başer KHC. Headspace-SPME and hydrodistillation of two fragrant Artemisia sp. Flavour Fragr J 20: 395-398, 2005.
  • Güven KC. Türkiye’nin A rtemisia türleri üzerinde çalışmalar II-Artemisia campestris L. Folia Farmasötika 5: 586-591, 1963.
  • Adams RP. Identifi cation of Esse ntial Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corporation. Illinois, USA; 2001.
  • Collins CM, Lyne PM. Microbiological Methods. Butterworths. London; 1989.
  • Bradshaw LJ. Laboratory Microbiology, 4th ed. Saunders. Fort Worth, Texas, USA; 1992.
  • National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disc Susceptibility Test, 6th ed. Approved Standard M2-A6. NCCLS. Villanova, PA; 1997.
  • Amarowicz R, Pegg RB, Rahimi-Mog haddam P et al. Free- radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem 84: 551- 562, 2004.
  • Re R, Pellegrini N, Proteggente A et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237, 1999.
  • Prieto P, Pineda M, Aguilar M. S pectrophotometric
  • quantitation of antioxidant capacity through the formation of
  • a phosphomolybdenum complex: specifi c application to the
  • determination of vitamin E. Anal Biochem 269: 337-341, 1999.
  • McDonald S, Prenzler PD, Antolovich M et al. Phenolic content and antioxidant activities of olive extracts. Food Chem 73: 73-84, 2001.
  • Chang CC, Yang MH, Wen HM. Estimation of total fl avonoid content in propolis by two complementary colorimetric methods. J of Food and Drug Anal 10: 178-182, 2002.
  • Baytop T. Farmakognozi II. İstan bul University Press. İstanbul; 1983: pp. 295-296.
  • Klaassen DC. Casarett & Davill’s T oxicology: Th e Basic Science of Poisons. McGraw Hill. New York; 2001.
  • Bellomaria B, Valentini G, Biondi E. Chemotaxonomy of Artemisia variabilis Ten. and A. campestris ssp. glutinosa (Ten.) Briq. et Cavill. (Asteraceae) from Italy. J Ess Oil Res 13: 90-94, 2001.
  • Singh HP, Mittal S, Kaur S et al. C hemical composition and antioxidant activity of essential oil from residues of Artemisia scoparia. Food Chem 114: 642-645, 2009.
  • Albayrak S, Aksoy A, Sağdıç O et al. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turk J Biol 34: 463-473, 2010.
  • Negro C, Tomassi L, Miceli A. Phenolic compounds and antioxidant activity from red grape marc extracts. Biosource Tech 87: 41-44, 2003.
  • Akrout A, El Jani H, Amouri S et al. Sc reening of antiradical and antibacterial activities of essential oils of Artemisia campestris L., Artemisia herba alba Asso, & Th ymus capitatus Hoff . et Link. growing wild in the southern of Tunisia. Recent Research in Science and Technology 2: 29-39, 2010.
  • Naili BM, Alghazeer RO, Saeh NA et al. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Asteraceae) and Ziziphus lotus (Rhamnacea). Arabian Journal of Chemistry 3: 79-84, 2010.
  • Coşar G, Çubukçu B, Melikoğlu G et al. Antimicrobial activity of Artemisia species growing in Turkey. J Fac Pharm İstanbul 30: 19-24, 1994.
  • Cha JD, Jeong MR, Jeong SI et al. Chemical composition and antimicrobial activity of the essential oils of Artemisia scoparia and A. capillaris. Planta Med 71: 186-190, 2005.
  • Ramezani M, Fazli-Bazza BS, Saghafi -Khadem F et al. Antimicrobial activity of four Artemisia species of Iran. Fitoterapia 75: 201-203, 2004.
  • Dülger B, Ceylan M, Alitsaous M et al. Artem isia absinthium L. (Pelin)’un antimikrobiayal aktivitesi. Turk J Biol 23: 377-384, 1999.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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