Koffi KOUAME, Aniekan İmo PETER, Edidiong Nnamso AKANG, Misturah ADANA, Roshila MOODLEY, Edwin Coleridge NAIDU, Onyemaechi Okpara AZU

Effect of long-term administration of Cinnamomum cassia silver nanoparticles on organs (kidneys and liver) of Sprague-Dawley rats

This study investigated the toxic effects of silver on the kidneys and livers of Sprague-Dawley rats after administering multiple doses of silver nanoparticles synthesized using extracts of Cinnamomum cassia (CcAgNPs). Twenty-four Sprague-Dawley rats (250 ± 20 g) were randomly assigned to four groups (A-D) of six animals per group and treated for 8 weeks. Group A was administered 200 mg/kg of Cinnamon Cassia extract (Cc), group B 5 mg/kg of CcAgNPs, group C 10 mg/kg of CcAgNPs, and group D normal saline. Body weight was measured weekly and fasting blood glucose was measured fortnightly. At the end of the experiment, animals were euthanized and organs (livers and kidneys) were fixed in neutral buffered formalin and processed for light microscopy (H&E). Body weight differences were significantly higher (P < 0.05) in the low-dose Cc group and the kidney to body weight ratio was not significant. Renal function analysis of proteins and ketones showed a significant increase in CcAgNP-treated rats (P < 0.05). Kidney and liver histology showed distortions in hepatocytes and sinusoidal linings with infiltrations especially in the higher dose groups. Kidney histology mirrored degenerative changes in glomerular and Bowman?s capsules with fibrillary mesangial interstitium. CcAgNPs impairs renal and hepatic morphology and function after a long period of administration.

Keywords:

Histology, nanomedicine, toxicity, degenerative, congestion,

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Adeyemi OS, Adewumi I, Faniyan TO (2015). Silver nanoparticles influenced rat serum metabolites and tissue morphology. J Basic Clin Physiol Pharmacol 26: 355-361.
Ahmed, S, Ahmad, M, Swami, B. L, Ikram, S (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Journal of Advanced Research 7: 17-28.
Ali ZA, Yahya R, Sekaran SD, Puteh R (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Adv Mater Sci Eng 2016: 4102196.
Amin YM, Hawas AM, El-Batal A, Elsayed SHHE (2015). Evaluation of acute and subchronic toxicity of silver nanoparticles in normal and irradiated animals. Br J Pharmacol Toxicol 6: 22- 38.
Anderson RA (2008). Chromium and polyphenols from cinnamon improve insulin sensitivity: plenary lecture. P Nutr Soc 67: 48- 53
Ansari S, Farha Islam M (2012). Influence of nanotechnology on herbal drugs. A review. J Adv Pharm Technol Res 3: 142.
Azu O (2015). Testicular morphology in spontaneously hypertensive rat model: oxidant status and stereological implications. Andrologia 47: 123-137.
Connor EE, Mwamuka J, Gole A, Murphy C J, Wyatt M D (2005). Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 1: 325-327.
Daisy P, Saipriya K (2012). Biochemical analysis of Cassia fistula aqueous extract and phytochemically synthesized gold nanoparticles as hypoglycemic treatment for diabetes mellitus. Int J Nanomedicine 7: 1189.
Day W, Hunt J, McGiven A (1976). Silver deposition in mouse glomeruli. Pathology 8: 201-204.
De Jong WH, Van Der Ven LT, Sleijffers A, Park MV, Jansen EH, Van Loveren H, Vandebriel RJ (2013). Systemic and immunotoxicity of silver nanoparticles in an intravenous 28 days repeated dose toxicity study in rats. Biomaterials 34: 8333-8343.
Dos Santos CA, Seckler MM, Ingle AP, Gupta I, Galdiero S, Galdiero M, Rai, M (2014). Silver nanoparticles: therapeutical uses, toxicity, and safety issues. J Pharm Sci 103: 1931-1944.
Elobeid MA (2016). Amelioration of streptozotocin induced diabetes in rats by eco-friendly composite nano-cinnamon extract. Pak J Zool 48: 645-650.
Fatima M, Zaidi NUSS, Amraiz D, Afzal F (2016). In vitro antiviral activity of Cinnamomum cassia and its nanoparticles against H7N3 influenza a virus. Journal of Microbiology and Biotechnology 26: 151-159.
Furchner J, Richmond C, Drake G (1968). Comparative metabolism of radionuclides in mammals-IV. Retention of silver-110m in the mouse, rat, monkey, and dog. Health Phys 15: 505-514.
Hussain S, Hess K, Gearhart J, Geiss K, Schlager J (2005). In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19: 975-983.
Hussain SM, Javorina AK, Schrand AM, Duhart HM, Ali SF, Schlager JJ (2006). The interaction of manganese nanoparticles with PC- 12 cells induces dopamine depletion. Toxicol Sci 92: 456-463.
Ismail OO, Isaac JA, Ugochukwu O, Oluwatosin OO, Aniekan PI, Edwin NC, Onyemaechi AO (2017). Impaired expression of testicular androgen receptor and collagen fibers in the testis of diabetic rats under HAART. The role of Hypoxis hemerocallidea . Folia Histochem Cyto 55: 149-158.
Jarvill-Taylor KJ, Anderson RA, Graves DJ (2001). A hydroxychalcone derived from cinnamon functions as a mimetic for insulin in 3T3-L1 adipocytes. J Am Coll Nutr 20: 327-336.
Khlebtsov N, Dykman L (2011). Biodistribution and toxicity of engineered gold nanoparticles: a review of in vitro and in vivo studies. Chem Soc Rev 40: 1647-1671.
Kim YS, Song MY, Park JD, Song KS, Ryu HR, Chung YH, Kelman BJ (2010). Subchronic oral toxicity of silver nanoparticles. Part Fibre Toxicol 7: 20.
Kumar A, Zhang X, Liang XJ (2013). Gold nanoparticles: emerging paradigm for targeted drug delivery system. Biotechnol Adv 31: 593-606.
Latendresse JR, Warbrittion AR, Jonassen H, Creasy DM (2002). Fixation of testes and eyes using a modified Davidson’s fluid: comparison with Bouin’s fluid and conventional Davidson’s fluid. Toxicol Pathol 30: 524-533.
Lee HS (2002). Inhibitory activity of Cinnamomum cassia bark- derived component against rat lens aldose reductase. J Pharm Pharm Sci 5 : 226-230
Luo Q, Wang SM, Lu Q, Luo J, Cheng YX (2013). Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose- induced mesangial cells. Molecules 18: 10930-10943.
Ogedengbe, O, Jegede A, Onanuga I, Offor U, Peter A, Akang E, Azu O (2018). Adjuvant potential of virgin coconut oil extract on antiretroviral therapy-induced testicular toxicity. An ultrastructural study. Andrologia 50: e12930.
Okafor F, Janen A, Kukhtareva T, Edwards V, Curley M (2013). Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int J Environ Res Public Health 10: 5221-5238.
Pandey S, Mewada A, Thakur M, Shah R, Oza G, Sharon M (2013). Biogenic gold nanoparticles as fotillas to fire berberine hydrochloride using folic acid as molecular road map. Mater Sci Eng C 33: 3716-3722.
Peter AI, Naidu EC, Akang E, Ogedengbe OO, Offor U, Rambharose S, Azu OO (2018). Investigating organ toxicity profile of tenofovir and tenofovir nanoparticle on the liver and kidney: experimental animal study. Toxicol Res 34: 221.
Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S (2007). Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli . Nanomed-Nanotechnol 3: 168-171.
Shakeel M, Jabeen F, Iqbal R, Chaudhry AS, Zafar S, Ali M, Asghar MS (2018). Assessment of titanium dioxide nanoparticles (TiO 2-NPs) induced hepatotoxicity and ameliorative effects of Cinnamomum cassia in Sprague-Dawley rats. Biol Trace Elem Res 182: 57-69.
Shankar S, Teng X, Li G, Rhim JW (2015). Preparation, characterization, and antimicrobial activity of gelatin/ZnO nanocomposite films. Food Hydrocolloid 45: 264-271.
Shankar T, Karthiga P, Swarnalatha K, Rajkumar K (2017). Green synthesis of silver nanoparticles using Capsicum frutescence and its intensified activity against E. coli. Resour Effic Technol 3: 303-308
Sulaiman F, Akanji M, Oloyede H, Sulaiman A, Olatunde A, Joel E, Quadri A (2015). Oral exposure to silver/gold nanoparticles: status of rat lipid profile, serum metabolites and tissue morphology. J Med Sci 15: 71.
Sulaiman FA, Adeyemi OS, Akanji MA, Oloyede HOB, Sulaiman AA, Olatunde A, Muritala H (2015). Biochemical and morphological alterations caused by silver nanoparticles in Wistar rats. Journal of Acute Medicine 5: 96-102.
Vasanth SB, Kurian GA (2017). Toxicity evaluation of silver nanoparticles synthesized by chemical and green route in different experimental models. Artif Cells Nanomed Biotechnol 45: 1721-1727.
Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y (1992). Evolution and ecology of influenza A viruses. Microbiol Rev 56: 152-179.
World Health Organization (2016). Global Report on Diabetes. Geneva, Switzerland: WHO Press.
Yun JW, You JR, Kim YS, Kim SH, Cho EY, Yoon JH, Kim HC (2018). In vitro and in vivo safety studies of cinnamon extract ( Cinnamomum cassia ) on general and genetic toxicology. Regul Toxicol Pharm 95: 115-123.
Zhang XF, Choi YJ, Han JW, Kim E, Park JH, Gurunathan S, Kim JH (2015). Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells. Int J Nanomedicine 10: 1335.
Zhou X, Wang B, Zhu L, Hao S (2012). A novel improved therapy strategy for diabetic nephropathy: targeting AGEs. Organogenesis 8: 18-21.