The effect of hot water, 1-MCP, and lovastatin on fresh-cut apples after long-term controlled atmosphere storage

This research was carried out over two years to determine the quality changes in fresh-cut apple slices processed after hot water, 1-methylcyclopropene 1-MCP , and lovastatin treatments followed by controlled atmosphere CA storage. Apples harvested at the optimum stage were immediately transferred to the postharvest laboratory by a refrigerated vehicle. Intact apples were exposed to three treatments: dipping into hot water 50 °C for 60 s , treating with lovastatin 1.25 mmol L-1 , and treating with 1-MCP 1 µL L-1 . Treated apples and the control group were stored in CA storage 2% O2 + 2% CO2 for 10 months. After long-term storage, the apples, sliced and packaged in plastic boxes, were stored at 0 °C and 90 ± 5% relative humidity RH for 14 days. Weight loss, soluble solid content, flesh firmness and color, titratable acidity, respiration rate, microbial count, and ethylene synthesis were determined during storage. As a result, a global beneficial effect of 1-MCP on sliced apples was observed, although its affirmative effect on ethylene synthesis and respiration rate vanished after 10 months of storage. 1-MCP-treated slices had better results in terms of microbial count, flesh firmness, external appearance, and weight loss compared to other applications. Lovastatin decreased the ethylene synthesis and respiration rate and was the best treatment to maintain the flavor of apple slices. Hot water and control treatments gave similar results for fruit quality and microbial activity. After long-term cold storage 10 months in CA, fresh-cut apple slices could be stored for 7 days with good quality at 0 °C and 90 ± 5% RH.

___

  • Aguayo E, Jackman CR, Stanley R, Woolf A (2015). Hot water treatment in combination with calcium ascorbate dips increases bioactive compounds and helps to maintain fresh-cut apple quality. Postharvest Biology and Technology 110: 158- 165. doi: 10.1016/j.postharvbio.2015.07.001
  • Artes F, Gomez PA, Artes-Hernandez F (2007). Physical, physiological and microbial deterioration of minimally fresh processed fruits and vegetables. Food Science and Technology International 13: 177-188. doi: 10.1177/1082013207079610
  • Bayındır D, Dilmaçünal T, Koyuncu MA, Onursal CE (2013). Effects of dipping treatments on the quality of fresh-cut ‘pink lady’ apples during cold storage. Acta Horticulturae 1012: 1057- 1063. doi: 10.17660/ActaHortic.2013.1012.143
  • Calderon-Lopez B, Bartsch JA, Lee C, Watkins BC (2005). Cultivar effects on quality of fresh cut apple slices from 1-MCP treated apple fruit. Journal of Food Science 70 (3): 221-227. doi: 10.1111/j.1365-2621.2005.tb07161.x
  • Chen C, Hu V, He Y, Jiang A, Zhang R (2016). Effect of citric acid combined with UV-C on the quality of fresh-cut apples. Postharvest Biology and Technology 111: 126-131. doi: 10.1016/j.postharvbio.2015.08.005
  • Dea S, Brecht JK, Nunes MCN, Baldwin, EA (2010). Quality of freshcut ‘Kent’ mango slices prepared from hot water or non-hot water-treated fruit. Postharvest Biology and Technology 56: 171-180. doi: 10.1016/j.postharvbio.2010.01.007
  • DeLong JM, Prange RK (2003). Superficial scald a postharvest oxidative stress disorder. In: Hodges DM (editor). Postharvest Oxidative Stress in Horticultural Crops. USA: Philadelphia, PA, USA: Haworth Press, pp. 91-112.
  • Dilmacunal T, Erbas D, Koyuncu MA, Onursal CE, Kuleasan H (2014). Efficacy of some antimicrobial treatments compared to sodium hypochlorite on physical, physiological and microbial quality of fresh-cut melons (Cucumis melo L. var. inodorus). LWT – Food Science and Technology 59: 1146-1151. doi: 10.1016/j.lwt.2014.07.033
  • Djioua T, Charles F, Lopez-Lauri F, Filgueiras H, Coudret A et al. (2009). Improving the storage of minimally processed mangoes (Mangifera indica L.) by hot water treatments. Postharvest Biology and Technology 52: 221-226. doi: 10.1016/j. postharvbio.2008.10.006
  • Georgoudaki T, Nanos GD (2015). Quality of fresh-cut apples treated at harvest with 1-MCP. Acta Horticulturae 1079: 441-446. doi: 10.17660/.2015.1079.57
  • Hartig K, Beck E (2005). Assessment of lovastatin application as tool in probing cytokinin-mediated cell cycle regulation. Physiologia Plantarum 125: 260-267. doi: 10.1111/j.1399-3054.2005.00556.x
  • Jeziorek K, Wonzi AK, Tolmala K (2010). Response of “Golden Delicious” apples to postharvest application of 1-Methylcyclopropene (1-MCP) in conditions of normal and controlled atmosphere. Journal of Fruit and Ornamental Plant Research 18: 223-237.
  • Jiang Y, Joyce D (2002). 1-Methylcyclopropene treatment effects on intact and fresh-cut apple. Journal of Horticultural Science & Biotechnology 77 (1): 19-21. doi: 10.1080/14620316.2002.11511450
  • Ju Z, Curry EA (2000a). Lovastatin inhibits α-farnesene biosynthesis and scald development in Delicious and ‘Granny Smith’ apples and ‘d’Anjou’ pears. Journal of American Society of Horticultural Science 125: 626-629. doi: 10.21273/JASHS.125.5.626
  • Ju Z, Curry EA (2000b). Lovastatin inhibits a-farnesene synthesis without affecting ethylene production during fruit ripening in ‘Golden Supreme’ apples. Journal of American Society of Horticultural Science 125: 105-110. doi: 10.21273/ JASHS.125.1.105
  • Koyuncu MA, Erbas D, Onursal CO, Secmen T, Guneyli A et al. (2019). Postharvest treatments of salicylic acid, oxalic acid and putrescine influences bioactive compounds and quality of pomegranate during controlled atmosphere storage. Journal of Food Science Technology 56: 350-359. doi: 10.1007/s13197- 018-3495-1
  • Koyuncu MA, Koyuncu F, Dilmaçünal T, Kuleaşan H, Çetinbaş M et al. (2010). Cold storage of fresh-cut “Granny Smith” apples. Acta Horticulturae 876: 307-319. doi: 10.17660/ ActaHortic.2010.876.41
  • Kupferman E (2001). Storage Scald of Apples. Othello, WA, USA: Washington State University.
  • Li G, Tan M, Cheng F, Liu X, Qi S et al. (2018). Molecular role of cytokinin in bud activation and outgrowth in apple branching based on transcriptomic analysis. Plant Molecular Biology 98: 261-274. doi: 10.1007/s11103-018-0781-2
  • Li J, Guo Y, Li Z, Lin Y, Liu L et al. (2015). Supercritical carbon dioxide and hexane extraction of wax from apple peel pomace: content, composition, and thermal properties. Separation Science and Technology 50: 2230-2237. doi: 10.1080/01496395.2015.1020951
  • Li L, Ban Z, Li X, Xue T (2014). Effect of 1-methylcyclopropene and calcium chloride treatments on quality maintenance of ‘Lingwu Long’ Jujube fruit. Journal of Food Science Technology 51 (4): 700-707. doi: 10.1007/s13197-011-0545-3
  • López ML, Espinoza AV, Reyna RA, Valenzuela JL, García MV (2013). Combined effect of hot water dipping treatment, N-acetylcysteine and calcium on quality retention and enzymatic activity of fresh-cut apple. Journal of Food, Agriculture & Environment 11 (2): 243-248.
  • Moghadam JF, Ebadi H (2012). The effect of hot water treatments on gray mold and physicochemical quality of kiwifruit during storage. Journal of Ornamental and Horticultural Plants 2: 73-82.
  • Muthuswamy S, Rupasinghe HPV, Stratton GW (2008). Antimicrobial effect of cinnamon bark extract on Escherichia coli O157:H7, Listeria innocua and fresh cut apple slices. Journal of Food Safety 28: 534-549. doi: 10.1111/j.1745-4565.2008.00129.x
  • Parmar N, Singh N, Kaur A, Thakur S (2017). Comparison of color, anti-nutritional factors, minerals, phenolic profile and protein digestibility between hard-to-cook and easy-to-cook grains from different kidney bean (Phaseolus vulgaris) accessions. Journal of Food Science Technology 54 (4): 1023-1034. doi: 10.1007/ s13197-017-2538-3
  • Plotto A, Bai J, Baldwin EA, Brecht JK (2003). Effect of pretreatment of intact ‘Kent’ and ‘Tommy Atkins’ mangoes with ethanol vapor, heat or 1-Methylcyclopropene on quality and shelf life of freshcut slices. Proceedings of the Florida State Horticultural Society 116: 394-400.
  • Rupasinghe HPV, Murry DP, Deell JR, Odumeru J (2005). Influence of 1-methylcyclopropene and natureseal on the quality of fresh-cut ‘Empire’ and ‘Crispin’ apples. Journal of Food Quality 28: 289- 307. doi: 10.1111/j.1745-4557.2005.00035.x
  • Saftner AR, Abbott JA, Bgagwat AA, Vinyard TB (2005). Quality measurement of intact and fresh-cut slices of Fuji, Granny Smith, Pink Lady and Goldrush apples. Journal of Food Science 70 (5): 317-324. doi: 10.1111/j.1365-2621.2005.tb09985.x
  • Siddiq M, Harte JB, Beaudry RM, Dolan KD, Singh SP et al. (2014). Physicochemical properties of whole fruit and sensory quality of fresh-cut apples pre-treated with 1-Methylcyclopropene (1- MCP). International Journal of Food Properties 17: 1081-1092. doi: 10.1080/10942912.2012.687799
  • Tan M, Li G, Qi S, Liu X, Chen X et al. (2018). Identification and expression analysis of the IPT and CKX gene families during axillary bud outgrowth in apple (Malus domestica Borkh.). Gene 651: 106-117. doi: 10.1016/j.gene.2018.01.101
  • Tardelli F, Guidi L, Massai R, Toivonen PMA (2013). Effects of 1-methylcyclopropene and post-controlled atmosphere air storage treatments on fresh-cut Ambrosia apple slices. Journal of the Science of Food Agriculture 93: 262-270. doi: 10.1002/ jsfa.5750
  • Tatsuki M, Endob A, Ohkawa H (2007). Influence of time from harvest to 1-MCP treatment on apple fruit quality and expression of genes for ethylene biosynthesis enzymes and ethylene receptors. Postharvest Biology and Technology 43: 28-35. doi: 10.1016/j. postharvbio.2006.08.010
  • Varoquaux P, Lecendre I, Varoquaux F (1990). Changes in firmness of kiwifruit after slicing. Sciences des Aliments 10: 127-139. Watkins CB (2006). The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnology Advances 24: 389-409. doi: 10.1016/j.biotechadv.2006.01.005
  • Zheng WW, Chun IJ, Hong SB, Zang YX (2014). Quality characteristics of fresh-cut ‘Fuji’ apple slices from 1-methylcyclopropene, calcium chloride and rare earth-treated intact fruits. Scientia Horticulturae 173: 100-105. doi: 10.1016/j.scienta.2014.04.025