Yeşim YALÇIN MENDİ, Muzaffer İPEK, Nihal BUZKAN, Yıldız AKA KAÇAR, Sebahattin ÇÜRÜK

Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Two different types of explant (proximal and flamingo-bill) from Emphasis seedlings, a hybrid cultivar of bottle gourd (Lagenaria siceraria (Molina) Stand.), germinated under dark and light conditions were cultured on 9 regeneration MS media containing various combinations of BA (0, 1.0, and 2.0 mg l-1) and IAA (0, 0.25, and 0.5 mg l-1). Comparison of the explant types showed that the flamingo-bill type explant had better shoot formation than did the proximal explant. The MS medium containing 1 mg l-1 of BA was optimal for shoot formation capacity when flamingo-bill type explants germinated in the dark (44%) and light (36%) were used. Histological analysis showed that explant cell division began after 3 days in regeneration medium and formation of primordium was observed in the tissues in culture between days 5 and 7. Differentiation of meristimatic structures was first observed after 9 days and development completed after 9-12 days in the culture.

Anahtar Kelimeler:

Bottle Gourd, Lagenaria siceraria, regeneration, histology, explant type

Regeneration and Histological Analysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.)

Keywords:

Bottle Gourd, Lagenaria siceraria, regeneration, histology, explant type,

PDF

___

Abrie, A.L and J. van Staden. 2001. Development and regeneration protocols for selected cucurbit cultivars. Plant Growth Regulation. 35: 263-267.
Ananthakrishnan, G., X. Xia, C. Elman, S. Singer, H.S. Paris, A. Gal-On and V. Gaba. 2003. Shoot production in squash (Cucurbita pepo) by in vitro organogenesis. Plant Cell Rep. 21: 739-746.
Chee, P.P. 1991. Plant regeneration from cotyledons Cucumis melo topmark. HortSci. 26: 908-910.
Choi, P.S., W.Y. Soh, Y.S. Kim, O.J. Yoo and J.R. Liu. 1994. Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens. Plant Cell Rep. 13: 344-348.
Compton, M.E. 1999. Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon. Plant Cell Tissue and Organ Cult. 58: 185-188.
Dabauza, M., M. Bordas, A. Salvador and L.A. Roig. 1997. Plant regeneration and Agrobacterium-mediated transformation of cotyledon explants of Citrullus colocynthis (L.) Schrad. Plant Cell Rep. 16: 888-892.
Dong, J.Z and S.R. Jia. 1991. High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad). Plant Cell Rep. 9: 559-562.
Evans, D.A., W.R. Sharp and C.E. Flick. 1981. Plant regeneration from cell cultures. Hort. Rev. 214-314.
Ezura, H., H. Amagai, K. Yoshiok and K. Oosawa. 1990. Highly frequent appearance of tetraploidy in regenerated plants, a universal phenomenon, in tissue culture of melon (Cucumis Melo L.). Plant Sci. 85: 209-213.
Food and Agriculture Organization (FAO). 2005. Statistics Database (Agriculture Data) 2001. On internet http:/apps.fao.org.
Ficcadenti, N and G.L. Rotino. 1995. Genotype and medium affect shoot regeneration of melon. Plant Cell, Tissue Org Cult. 40: 293-295.
Gaba, V., E. Schlarman, C. Elman, O. Sagee, A.A Watad and D.J. Gray. 1999. In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons. In Vitro Cellular and Developmental Biology-Plant. 35: 1-7.
Han, J.S., D.G. Oh, I.G. Mok, H.G. Park and C.K. Kim. 2004. Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep. 23: 291-296.
Han, J.S., C.K. Kim, S.H. Park, K.D. Hirschi and I.G. Mok. 2005. Agrobacterium-mediated transformation of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep. 23: 692-698.
Hartmann, H.T., D.E. Kester, F. Davies and R.L. Geneve. 1997. Plant propagation: principles and practices, 6th ed. Prentice-Hall, Inc. Englewood Cliffs, NJ.
Lee, Y.K., W.I. Chung and H. Ezura. 2003. Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch.). Plant Sci. 164: 413-418.
Lee, J.M. 1994. Cultivation of grafted vegetables I. Current status, grafting methods, and benefits. HortSci. 29: 235-239.
Lee, J.M. 2003. Advances in vegetable grafting. Chronica Horticulturae. 43:13-19.
Mohiuddin, A.K.M., M.K.U. Chowdhury, Z.C. Abdullah and S. Napis. 1997. Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tiss. Org. Cult. 51: 75-78.
Murashige, T and F. Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant. 15: 473-497.
Nishibayashi, S., H. Kaneko and T. Hayakawa. 1996. Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant Cell Rep. 15: 809-814.
Pal, S.P., I. Alam, M. Anisuzzaman, K.K. Sarker, S.A. Sharmin and M.F. Alam. 2007. Indirect organogenesis in summer squash (Cucurbita pepo L.). Turk. J. Agric. For. 31: 63-70.
Pozueta-Romero, J., G. Houlne, L. Canas, R. Schantz and J. Chamarro. 2001. Enhanced regeneration of tomato and pepper seedling explants for Agrobacterium-mediated transformation. Plant Cell, Tissue Org Cult. 67: 173-180.
Rahman, S.M., M. Hossaın, R. Islam and O.I. Joarder. 1993. Plant regeneration from internode segments of Cucurbita maxima Duch. x Cucurbita moschata Duch. Curr Sci. 65: 562-564.
Robinson, R.W and D.S. Decker-Walters. 1997. Cultural requirements. In Cucurbits. CAB International; Wallingford, U.K.
Sarowar, S., H.Y. Oh, N.I. Hyung, B.W. Min, C.H. Harn, S.K. Yang, S.H. Ok and J.S. Shin. 2003. In vitro propagation of a Cucurbita interspecific hybrid cultivar-a root stock plant. Plant Cell, Tissue Org Cult. 75: 179-182.
Oridate, T., H. Atsumi, S. Ito and H. Araki. 1992. Genetic difference in somatic embryogenesis from seeds in melon (Cucumis melo L.) Plant Cell, Tissue Org Cult. 29: 27-30.
Yalcin-Mendi, N.Y., M. Ipek, H. Kacan, S. Curuk, N. Sari, S. Cetiner and V. Gaba. 2003a. Histological analysis of regeneration in watermelon. J. Biochemistry and Biotech. 12: 147-150.
Yalcin-Mendi, N.Y., H. Kacan, Y. Aka-Kacar and S. Cetiner. 2003b. Morphologic and histologic analysis of regeneration in some melon varieties, Biotechnology & Biotechnological Equip. 1: 44-49.