Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment

Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment

This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves. Following foliar spray at three growth stages (40, 50, and 60 days) with selected PGRs, namely humic acid (HA), Moringa oleifera leaf extract (MOLE), and 6-benzylamino purine (6-BAP), and their mixed form, the samples of spinach leaves were harvested. The analysis of foliar-treated leaves revealed the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45 72.32 µg/g FW, 2.41 29.13 ng/g FW, 3.21 22.45 µg/g FW, 4.173 19.700 mg/g FW, and 0.500 1.260 mg/g FW, respectively. The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from 6.59 to 14.49 mg GAE/g DM, from 0.426 to 1.944 (10.0 mg/ mL extract concentration), and from 0.506 to 1.073 µg/mL, respectively. HPLC analysis showed that foliar application of PGRs also improved the content of individual phenolic acids in the leaves as compared with the controls. Overall, it was concluded that, although the use of the PGRs acted as an elicitor with respect to each growth phase and exhibited significant differences (P < 0.05) among treatments, the leading effect was exhibited by the MOLE treatment, proving it to be a better enhancer of phenolic antioxidants and other biochemicals.

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  • Abdalla MM (2013). The potential of Moringa oleifera extract as a biostimulant in enhancing the growth, biochemical and hormonal contents in rocket (Erucavesicaria subsp. sativa) plants. Int J Plant Physiol Biochem 5: 42–49.
  • Arshad S, Shafqat A (2012). Food security indicators, distribution and techniques for agriculture sustainability. Int J Appl Sci Tech 2: 137–147.
  • Asghari M, Aghdam MS (2010). Impact of salicylic acid on postharvest physiology of horticultural crops. Trends Food Sci Tech 21: 502–509. Aslam M, Sultana B, Ali S, Khalil-ur-Rehman (2013). Alteration in antioxidant potential of Spinacia oleracea in response to selected plant growth regulators. Pak J Agric Sci 50: 699–706.
  • Avenson T, Cruz J, Kanazawa A, Kramer, D (2005). Regulating the proton budget of higher plant photosynthesis. P Natl Acad Sci USA 102: 9709– 9713.
  • Basra SMA, Iftikhar MN, Afzal I (2011). Potential of moringa (Moringa oleifera) leaf extract as priming agent for hybrid maize seeds. Int J Agric Bio 13: 1006–1010.
  • Bates L, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207. Bozin B, Mimica-Dukic N, Simin N, Anackov G (2006).
  • Characterization of the volatile composition of essential oil of some Lamiaceae species and the antimicrobial and antioxidant activities of the entire oils. J Agric Food Chem 54: 1822–1828.
  • Bradford M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
  • Cakmak I, Horst WJ (1991). Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plantarum 83: 463–468.
  • Ceconi C, Boraso A, Cargnoni A, Ferrari R (2003). Oxidative stress in cardiovascular disease: myth or fact? Arch Biochem Biophys 420: 217–221.
  • Chaovanalikit A, Wrolstad RE (2004). Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties. Food Chem Toxicol 69: 67–72.
  • Chaudhry KM, Muhammad S, Ashraf I (2006). Alternative extension approaches to technology dissemination for sustainable agriculture in the Punjab, Pakistan. Int J Agric Bio 8: 836–839.
  • Cheng Y, Tang ZS, Wang ZX, Yang F (2001). Research of physiology and heredity on barley of chlorophyll-deficient mutant. J Sich Teachers College (Nat Sci) 22: 131–133.
  • Chumark P, Khunawat P, Sanvarinda P, Phornchirasilp S, Morales S, Phivthong-Ngam NPL, Ratanachamnong P, Srisawat P, Pongrapeeporn K U (2008). The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. Leaves. J Ethnopharmacol 116: 439–446.
  • Cimrin KM, Turkmen O, Turan M, Tuncer B (2010). Phosphorus and humic acid application alleviate salinity stress of pepper seedlings. Afr J Biotech 9: 5845–5851.
  • Clapp CE, Chen Y, Hayes MHB, Cheng HH (2010). Plant growth promoting activity of humic substances. In: Swift, RS, Sparks KM, editors. Understanding and Managing Organic Matter in Soils, Sediments, and Waters. Madison, WI, USA: International Humic Science Society, pp. 243–255.
  • Del-Rio D, Stewart AJ, Pellegrini N (2005). A review of recent studies on malondialdehyde as toxic molecule and biological markers of oxidative stress. Nutr Metab Cardiovas Disorders 15: 316– 328.
  • Devi RG, Pandiyarajan V, Gurusaravanan P (2012). Alleviating effect of IAA on salt stressed Phaseolus mungo (L.) with reference to growth and biochemical characteristics. Recent Res Sci Tech 4: 22–24.
  • Djousse L, Arnett DK, Coon H, Province MA, Moore LL, Ellision RC (2004). Fruit and vegetable consumption and LDL cholesterol: the National Heart, Lung, and Blood Institute Family Heart Study. Am J Clin Nutr 79: 213–217.
  • Dumas Y, Dadomo M, Lucca GD, Grolier P (2003). Effects of environmental factors and agricultural techniques on antioxidant content of tomatoes. J Sci Food Agric 83: 369–382.
  • Ejaz B, Sajid ZA, Aftab F (2012). Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp., hybrid cv. HSF-under salt stress. Turk J Biol 36: 630–640.
  • Ekmekci Y, Terzioglu S (2005). Effects of oxidative stress induced by paraquat on wild and cultivated wheat. Pest Biochem Physiol 83: 69–81.
  • Ellnain-Wojtaszek M, Kurczynski Z, Kasprzak J (2001). Analysis of the content of flavonoids, phenolic acids as well as free radicals from Ginkgo biloba L. leaves during the vegetative cycle. Acta Pol Pharm 58: 205–209.
  • El-Shabasi MS, Mohamed SM, Mahfouz SA (2005). Effect of foliar spray with some amino acids on growth, yield and chemical composition of garlic plants. In: The 6th Arabian Conference for Horticulture, Ismailia, Egypt.
  • El-Shraiy AM, Hegazi AM (2009). Effect of acetylsalicylic acid, indole-3-bytric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L.). Aust J Basic Appl Sci 3: 3514– 3523.
  • Ezz El-Din AA, Khalil MY (2003). Effect of foliar fertilization on growth and yield on two Plantago species cultivated in Egypt. Egypt J Hortic 30: 227–237.
  • Farahat AA, Alsayed AA, El-Beltagi, HS, Mahfoud NM (2012). Impact of organic and inorganic fertilizers on nematode reproduction and biochemical alterations on tomato. Not Sci Biol 4: 48–55.
  • Heidari M, Mesri F (2008). Salinity effects compatible solute, antioxidants enzymes and ion content in three wheat cultivars. Pak J Biol Sci 11: 1385–1389.
  • Hounsome N, Hounsome B, Tomos D, Edwards-Jones G (2008). Plant metabolites and nutritional quality of vegetables. J Food Sci 73: R48–R65.
  • Hung HC, Joshipura KJ, Jiang R, Hu FB, Hunter D, Smith-Warner SA, Colditz GA, Rosner B, Spiegelman D, Willett WC (2004).
  • Fruit and vegetable intake and risk of major chronic disease. J Natl Cancer Inst 96: 1577–1584.
  • Jungklang J, Songklanakarin KS (2012). Effect of paclobutrazol on Patumma cv. Chiang Mai Pink under water stress. J Sci Tech 34: 361–366.
  • Keramat B, Kalantari KM, Arvin MJ (2009). Effects of methyl jasmonate in regulating cadmium induced oxidative stress in soybean plant (Glycine max L.). Afr J Biotech 3: 240–244.
  • Lanfer-Marquez UM, Barros RMC, Sinnecker P (2005). Antioxidant activity of chlorophylls and their derivatives. Food Res Int 38: 885–891.
  • Lim PO, Woo HR, Nam HG (2003). Molecular genetics of leaf senescence in Arabidopsis. Trends Plant Sci 8: 272–278.
  • Mahmoudi M, Samavat S, Mostafavi M, Khalighi A, Cherati A (2013). The effects of humic acid and proline on nutrient uptake of Actindia deliciosa cv. Hayward under salinity. Int Res J Appl Basic Sci 4: 1021–1025.
  • Manach C, Scalbert A, Morand C, Remesy C, Jimenez L (2004). Polyphenols: food sources and bioavailability. Am J Clinic Nut 79: 727–747.
  • Masoumzadeh BM, Imani AA, Khayamaim S (2012). Salinity stress effect on proline and chlorophyll rate in four beet cultivars. Anal Biol Res 3: 5453–5456.
  • Merillon JM, Liu D, Huguet F, Chenieux JC, Rideau M (1991). Effects of calcium entry blockers and calmodulin inhibitors on cytokinin-enhanced alkaloid accumulation in Catharanthus roseus cell cultures. Plant Physiol Biochem 29: 289–296.
  • Nagata M, Yamashita T (1992). Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. J Japan Soc Food Sci Tech 39: 925–928.
  • Nair SA, Singh V, Sharma TV (2002). Effect of plant growth regulators on yield and quality of gerbera under Bay Island conditions. Ind J Hortic 59: 100–105.
  • Nawaz K, Talat AI, Hussain K, Majeed A (2010). Induction of salt tolerance in two cultivars of sorghum (Sorghum bicolor L.) by exogenous application of proline at seedling stage. World Appl Sci J 10: 93–99.
  • Niakan M, Habibi A, Ghorbanli M (2012). Study of pix regulator effect on physiological responses in cotton plant. Anal Appl Biol Res 3: 5229–5235.
  • Ouzounidou G, Giannakoula A, Asfi M, Ilias I (2011). Differential responses of onion and garlic against plant growth regulators. Pak J Botany 43: 2051–2057.
  • Ouzounidou G, Ilias I, Giannakoula A, Papadopoulou P (2010). Comparative study on the effects of various plant growth regulators on growth, quality and physiology of Capsicum annuum L. Pak J Botany 42: 805–814.
  • Raffo A, Leonardi C, Fogliano V, Ambrosino P, Salucci M, Gennaro L, Bugianesi R, Giuffrida F, Quaglia G (2002). Nutritional value of cherry tomatoes (Lycopersicum esculentum cv. Naomi F1) harvested at different ripening stages. J Agric Food Chem 50: 6550–6556.
  • Rajanandh MG, Satishkumar MN, Elango K, Suresh B (2012). Moringa oleifera Lam. A herbal medicine for hyperlipidemia: a pre-clinical report. Asian Pac J Trop Dis 2: S790–S795.
  • Saruhan V, Kusvuran A, Babat S (2011). The effect of different humic acid fertilization on yield and yield components performances of common millet (Panicum miliaceum L.). Sci Res Essays 6: 663–669.
  • Saxena M, Saxena J, Pradhan A (2012). Flavonoids and phenolic acids as antioxidants in plants and human health. Int J Pharm Sci Rev Res 28: 130–134.
  • Stopari G, Maksimovi I (2008). The effect of cytokinins on the concentration of hydroxyl radicals and the intensity of lipid peroxidation in nitrogen deficient wheat. Cereal Res Communications 36: 601–609.
  • Sultana B, Anwar F, Przybylski R (2007). Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. Trees. Food Chem 104: 1106–1114.
  • Szabados L, Savoure A (2010). Proline: a multifunctional amino acid. Trends Plant Sci 15: 89–97.
  • Tokuşoğlu Ö, Ünal MK, Yıldırım Z (2003). HPLC–UV and GC–MS characterization of the flavonol aglycons quercetin, kaempferol and myrecetin in tomato and tomato pastes and other tomato– based products. Acta Chromatogr 13: 196–207.
  • Wahba HE, Ezz El-Din AA (2002). Growth, yield and essential oil response of Chrysanthemum coronarium L. to plant spacing and foliar micro-elements. Egypt J Hortic 29: 229–247.
  • Wang FH, Wang, GX, Li XY, Huang JL, Zheng JK (2008). Heredity, physiology and mapping of a chlorophyll content gene of rice (Oryza sativa L.). J Plant Physiol 165: 324–330.
  • Xu F, Cheng S, Zhu J, Zhang W, Wang Y (2011). Effects of 5-aminolevulinic acid on chlorophyll, photosynthesis, soluble sugar and flavonoids of Ginkgo biloba. Notulae Botanicae Horti Agrobotanici Cluj–Napoca 39: 41–47.
  • Yan SP, Zhang QY, Tang ZC, Su WA, Sun WN (2006). Comparative proteomic analysis provides new insights into chilling stress responses in rice. Molecular and Cellular Proteomics 5: 484– 550.
  • Yen GC, Duh PD, Chuang DY (2000). Antioxidant activity of anthraquinones and anthrone. Food Chem 70: 307–315.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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