Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos

Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different dura-tions; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. Survival of embryos was low (about 10%) or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages (13.3-23.3%) were obtained with about 40.4-77.3% of loss of fresh weight and 9.1-34.6% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days, Mg(NO 3 ) 2 .6H 2 O for 5 days or NaCl for 5-6 days, and with uncovered plates over ZnSO 4 .7H 2 O for 6-7 days or NH 3 SO 4 for 6-8 days. The highest rooting percentage (80.9%) in the dark were obtained with 56.2% of loss of fresh weight and 16.8% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days. To improve germination, both desiccation and GA 3 treatments were more effective than only desiccation or GA 3 treatments on germination. Germinating was best (46.0%) with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA 3 in the light.

Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos

Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different dura-tions; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. Survival of embryos was low (about 10%) or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages (13.3-23.3%) were obtained with about 40.4-77.3% of loss of fresh weight and 9.1-34.6% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days, Mg(NO 3 ) 2 .6H 2 O for 5 days or NaCl for 5-6 days, and with uncovered plates over ZnSO 4 .7H 2 O for 6-7 days or NH 3 SO 4 for 6-8 days. The highest rooting percentage (80.9%) in the dark were obtained with 56.2% of loss of fresh weight and 16.8% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days. To improve germination, both desiccation and GA 3 treatments were more effective than only desiccation or GA 3 treatments on germination. Germinating was best (46.0%) with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA 3 in the light.

Kaynak Göster

Bibtex @ { tbtkagriculture138781, journal = {Turkish Journal of Agriculture and Forestry}, issn = {1300-011X}, eissn = {1303-6173}, address = {}, publisher = {TÜBİTAK}, year = {2000}, volume = {24}, pages = {491 - 498}, doi = {}, title = {Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos}, key = {cite}, author = {Dumanoğlu, Hatice} }
APA Dumanoğlu, H . (2000). Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos . Turkish Journal of Agriculture and Forestry , 24 (4) , 491-498 .
MLA Dumanoğlu, H . "Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos" . Turkish Journal of Agriculture and Forestry 24 (2000 ): 491-498 <
Chicago Dumanoğlu, H . "Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos". Turkish Journal of Agriculture and Forestry 24 (2000 ): 491-498
RIS TY - JOUR T1 - Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos AU - Hatice Dumanoğlu Y1 - 2000 PY - 2000 N1 - DO - T2 - Turkish Journal of Agriculture and Forestry JF - Journal JO - JOR SP - 491 EP - 498 VL - 24 IS - 4 SN - 1300-011X-1303-6173 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Agriculture and Forestry Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos %A Hatice Dumanoğlu %T Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos %D 2000 %J Turkish Journal of Agriculture and Forestry %P 1300-011X-1303-6173 %V 24 %N 4 %R %U
ISNAD Dumanoğlu, Hatice . "Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos". Turkish Journal of Agriculture and Forestry 24 / 4 (Nisan 2000): 491-498 .
AMA Dumanoğlu H . Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos. Turkish Journal of Agriculture and Forestry. 2000; 24(4): 491-498.
Vancouver Dumanoğlu H . Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos. Turkish Journal of Agriculture and Forestry. 2000; 24(4): 491-498.
IEEE H. Dumanoğlu , "Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos", Turkish Journal of Agriculture and Forestry, c. 24, sayı. 4, ss. 491-498, Nis. 2000