Fahriye Şeyma ÖZCAN, Nihat ÖZCAN, Ahmet Abdullah US, Osman SAĞDIÇ

Comparison of extraction methods optimised by RSM for extraction of some bioactives from liquorice samples

This study compared the extraction efficiency of 3 different techniques, ultrasound-assisted extraction (UAE), microwaveassisted extraction (MAE), and the classical extraction method, in obtaining some bioactive and allergen compounds, namely liquiritin (LQ), glycyrrhizin (GL), glycyrrhetinic acid (GA), and carbenoxolone (CBX), from liquorice samples of Glycyrrhiza glabra grown in Turkey. Extraction parameters for each technique were optimised by the response surface methodology-central composite design approach. Concentrations of LQ, GL, GA, and CBX, which were selected as the responses of selected independent variables, were determined by liquid chromatography-electrospray ionisation tandem mass spectrometry. One-way variance analysis was performed to evaluate the significance of the results. MAE was revealed as the most effective technique for extracting LQ (mean: 8.52 mg g-1), GL (mean: 76.65 mg g-1), and GA (mean: 1138.84 μg g-1) from liquorice, while the classical technique was the most effective for obtaining CBX (mean: 5.75 μg g-1).

Keywords:

LC-ESI-MS/MS, liquorice, microwave, response surface methodology ultrasound,

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Ashfaq UA, Masoud MS, Nawaz Z, Riazuddin S (2011). Glycyrrhizin as antiviral agent against hepatitis C virus. Journal of Translational Medicine 9: 112. doi:10.1186/1479-5876-9-112
Baite TN, Das AB (2018). Extraction of glycyrrhizin from licorice using single screw extruder: process kinetics and stimulus response modeling. Separation Science and Technology 53 (3): 449-457. doi: 10.1080/01496395.2017.1391846
Bao B, Larsen K (2010). Glycyrrhiza Linnaeus. In: Wu Z, Raven PH (editors). Flora of China. Beijing, China: Science Press and Missouri Botanical Garden Press, pp. 509.
Bezerra MA, Santelli RE, Oliveira EP, Villar LS, Escaleira LA (2008). Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta 76: 965-977. doi: 10.1016/j.talanta.2008.05.019
Çetin Ö, Duran A, Martin E, Küçüködük M (2015). Karyological studies in some Glycyrrhiza (Fabaceae) taxa from Turkey. Caryologia 68 (3): 254-264. doi: 10.1080/00087114.2015.1033833
Chemat F, Rombaut N, Meullemiestre A, Turk M, Perino S et al. (2017). Review of Green Food Processing techniques. Preservation, transformation, and extraction. Innovative Food Science and Emerging Technologies 41: 357-377. doi: 10.1016/j. ifset.2017.04.016
Cherng JM, Lin HJ, Hung MS, Lin YR, Chan MH et al. (2006). Inhibition of nuclear factor kappa B is associated with neuroprotective effects of glycyrrhizic acid on glutamate-induced excitotoxicity in primary neurons. European Journal of Pharmacology 547: 10-21. doi: 10.1016/j.ejphar.2006.06.080
Chin YW, Jung HA, Liu Y, Su BN, Castoro JA et al. (2007). Antioxidant constituents of the roots and stolons of licorice (Glycyrrhiza glabra). Journal of Agriculture and Food Chemistry 55 (12): 4691-4697. doi: 10.1021/jf0703553
Das A, Mandal V, Mandal S (2013). Design of experiment approach for the process optimisation of microwave assisted extraction of lupeol from Ficus racemosa leaves using response surface methodology. Phytochemical Analysis 24: 230-247. doi: 10.1002/pca.2403
Gao XY, Zheng QY, Sun JN, Wang WQ, Yang XY (2011). Preparation of total licorice saponin and research on its hepatoprotective effect. Pharmacology and Clinics of Chinese Materia Medica 27: 79-81.
Guo Z, Zhao B, Li H, Miao S, Zheng B (2019). Optimization of ultrasound-microwave synergistic extraction of prebiotic oligosaccharides from sweet potatoes (Ipomoea batatas L.). Innovative Food Science and Emerging Technologies 54: 51-63. doi: 10.1016/j.ifset.2019.03.009
Hedayati A, Ghoreishi S (2015). Supercritical carbon dioxide extraction of glycyrrhizic acid from licorice plant root using binary entrainer: experimental optimization via response surface methodology. Journal of Supercritical Fluids 100: 209- 217. doi: 10.1016/j.supflu.2015.03.005
Hennell J, Lee S, Khoo C, Gray M, Bensoussan A (2008). The determination of glycyrrhizic acid in Glycyrrhiza uralensis Fisch. ex DC. (Zhi Gan Cao) root and the dried aqueous extract by LC–DAD. Journal of Pharmaceutical and Biomedical Analysis 47 (3): 494-500. doi: 10.1016/j.jpba.2008.01.037
Hough CAM, Parker KJ (editors) (1973). Developments in Sweeteners-1. London, UK: AJ Vlitos Ltd.
Huang T, Chen N, Wang D, Lai Y (2014). Infrared-assisted extraction coupled with high performance liquid chromatography (HPLC) for determination of liquiritin and glycyrrhizic acid in licorice root. Analytical Methods 6: 5986-5991. doi: 10.1039/ C4AY01024H
Jang S, Lee AY, Lee AR, Choi G, Kim HK (2017). Optimization of ultrasound-assisted extraction of glycyrrhizic acid from licorice using response surface methodology. Integrative Medicine Research 6 (4): 388-394. doi: 10.1016/j.imr.2017.08.003
Isbrucker R, Burdock G (2006). Risk and safety assessment on the consumption of licorice root (Glycyrrhiza sp.), its extract and powder as a food ingredient, with emphasis on the pharmacology and toxicology of glycyrrhizin. Regulatory Toxicology and Pharmacology 46 (3): 167-192. doi: 10.1016/j.yrtph.2006.06.002
Kaderides K, Papaoikonomou L, Serafim M, Goula AM (2019). Microwave-assisted extraction of phenolics from pomegranate peels: optimization, kinetics, and comparison with ultrasounds extraction. Chemical Engineering and Processing Process Intensification 137: 1-11. doi: 10.1016/j.cep.2019.01.006
Kitagawa I (2002). Licorice root: a natural sweetener and an important ingredient in Chinese medicine. Journal of Pure and Applied Chemistry 74 (7): 1189-1198. doi: 10.1351/pac200274071189
Lange D (1998). Europe’s Medicinal and Aromatic Plants: Their Use, Trade and Conservation. Cambridge, UK: Traffic International.
Li XH, Qi Y, Cai RL, Liu B, Song Y et al. (2010). Studies on the antiinflammatory mechanism of total saponins of radix Glycyrrhiza in vitro. Chinese Journal of Experimental Traditional Medical Formulae 16: 110-113.
Liao J, Qu B, Zheng N (2016). Extraction of glycyrrhizic acid from Glycyrrhiza uralensis using ultrasound and its process extraction model. Applied Sciences 6 (11): 319. doi: 10.3390/app6110319
Liu H, Sugimoto N, Akiyama T, Maitani T (2000). Constituents and their sweetness of food additive enzymatically modified licorice extract. Journal of Agricultural and Food Chemistry 48 (12): 6044-6047. doi: 10.1021/jf000772e
Mandal V, Mandal SC (2010). Design and performance evaluation of a microwave based low carbon yielding extraction technique for naturally occurring bioactive triterpenoid: oleanolic acid. Biochemical Engineering Journal 50 (1): 63-70. doi: 10.1016/j. bej.2010.03.005
Mandal V, Mohan Y, Hemalatha S (2007). Microwave assisted extraction–an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews 1: 7-18.
Mukhopadhyay M, Panja P (2008). A novel process for extraction of natural sweetener from licorice (Glycyrrhiza glabra) roots. Separation and Purification Technology 63 (3): 539-545. doi: 10.1016/j.seppur.2008.06.013
Pan X, Liu H, Jia G, Shu Y (2000). Microwave-assisted extraction of glycyrrhizic acid from licorice root. Biochemical Engineering Journal 5 (3): 173-177. doi: 10.1016/S1369-703X(00)00057-7
Richter BE, Jones BA, Ezzell JL, Porter NL, Avdalovic N et al. (1996). Accelerated solvent extraction: a technique for sample preparation. Analytical Chemistry 68 (6): 1033-1039. doi: 10.1021/ac9508199
Roselló-Soto E, Galanakis CM, Brnčić M, Orlien V, Trujillo FJ et al. (2015). Clean recovery of antioxidant compounds from plant foods, by-products and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends in Food Science & Technology 42 (2): 134-149. doi: 10.1016/j. tifs.2015.01.002
Shabkhiz MA, Eikani MH, Sadr ZB, Golmohammad F (2016). Superheated water extraction of glycyrrhizic acid from licorice root. Food Chemistry 210: 396-401. doi: 10.1016/j. foodchem.2016.05.006
Tao W, Duan J, Zhao R, Li X, Yan H et al. (2013). Comparison of three officinal Chinese pharmacopoeia species of Glycyrrhiza based on separation and quantification of triterpene saponins and chemometrics analysis. Food Chemistry 141 (3): 1681-1689. doi: 10.1016/j.foodchem.2013.05.073
Vilkhu K, Mawson R, Simons L, Bates D (2008). Applications and opportunities for ultrasound assisted extraction in the food industry-A review. Innovative Food Science & Emerging Technologies 9 (2): 161-169. doi: 10.1016/j.ifset.2007.04.014
Wang Y, Li R, Jiang ZT, Tan J, Tang SH et al. (2018). Green and solventfree simultaneous ultrasonic-microwave assisted extraction of essential oil from white and black peppers. Industrial Crops and Products 114: 164-172. doi: 10.1016/j.indcrop.2018.02.002
Wang YC, Yang YS (2007). Simultaneous quantification of flavonoids and triterpenoids in licorice using HPLC. Journal of Chromatography B 850 (1): 392-399. doi: 10.1016/j. jchromb.2006.12.032
Wen C, Zhang J, Zhang H, Dzah CS, Zandile M et al. (2018). Advances in ultrasound assisted extraction of bioactive compounds from cash crops-A review. Ultrasonics Sonochemistry 48: 538-549. doi: 10.1016/j.ultsonch.2018.07.018
Yan MM, Liu W, Fu YJ, Zu YG, Chen CY et al. (2010). Optimisation of the microwave-assisted extraction process for four main astragalosides in Radix Astragali. Food Chemistry 119 (4): 1663-1670. doi: 10.1016/j.foodchem.2009.09.021
Yang L, Li LL, Liu TT, Zu YG, Yang FJ et al. (2013). Development of sample preparation method for isoliquiritigenin, liquiritin, and glycyrrhizic acid analysis in licorice by ionic liquidsultrasound based extraction and high-performance liquid chromatography detection. Food Chemistry 138 (1): 173-179. doi: 10.1016/j.foodchem.2012.10.059
Zhang B, Yang R, Liu CZ (2008). Microwave-assisted extraction of chlorogenic acid from flower buds of Lonicera japonica Thunb. Separation and Purification Technology 62 (2): 480-483. doi: 10.1016/j.seppur.2008.02.013
Zhao Z, Xu X, Ye Q, Dong L (2013). Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity. International Journal of Biological Macromolecules 59: 290-294. doi: 10.1016/j.ijbiomac.2013.04.067
Zheng YF, Qi LW, Cui XB, Peng GP, Peng YB et al. (2010). Oleananetype triterpene glucuronides from the roots of Glycyrrhiza uralensis Fischer. Planta Medica 76 (13): 1457-1463. doi: 10.1055/s-0029-1240907