Zihni DEMİRBAĞ, İsmail DEMİR, Remziye NALÇACIOĞLU, Lida GHOLİZAD MOHAMMAD

A highly efective nucleopolyhedrovirus against Malacosoma spp. (Lepidoptera: Lasiocampidae) from Turkey: isolation, characterization, phylogeny, and virulence

A nucleopolyhedrovirus (NPV) was isolated from diseased larvae of the eastern tent caterpillar, Malacosoma americanum(Fabricius), collected from the northeastern part of Turkey. Light and electron microscopic observations showed that this virus is amultiple nucleopolyhedrovirus (MNPV). Te dimensions of the polyhedral inclusion bodies varied from 1.0 to 2.1 µm, and the sizesof the virions were also measured to be approximately 194.5 nm. Restriction analysis of the new isolate and ManeNPV-T2 (previouslyisolated from M. neustria larvae) showed that the new virus is closely related to ManeNPV-T2. Furthermore, sequence and phylogeneticanalysis of polh and lef-8 also revealed a high degree of homology to M. neustria NPV. Our results indicate that the new virus is anotherisolate of ManeNPV that infects M. americanum larvae naturally. Terefore, it was named ManeNPV-T3. Te virulence of ManeNPV-T3,which was tested against the third instar of M. americanum and M. neustria larvae under laboratory conditions, indicated that 0.6 ×10 5 occlusion bodies per larvae caused 100% mortality within 10 days. Te same dose also showed viral infection in Malacosomacastrensis, Plodia interpunctella , Taumetopoea pityocampa, and Galleria mellonella larvae causing 65%, 60%, 35%, and 25% mortalities,respectively. Te fndings indicate that ManeNPV-T3 is a promising microbial agent for the biological control of Malacosoma species.

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Abbott WS (1925). A method of computing the efectiveness of an insecticide. J Econ Entomol 18: 265 267.
Ackermann HW, Smirnof WA (1983). A morphological investigation of 23 baculoviruses. J Invertebr Pathol 41: 269 280.
Biliotti E (1955). Survie des larves endophages de tachinaires à une mort prématurée de leur hôte par maladie. CR Acad Sci Paris 240: 10211023 (article in French).
Demir İ, Gürel N, Nalçacıoğlu R, İnce İA, Demirbağ Z (2009a). Productive replication of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) in Md203 cell line. Turk J Biol 33: 239248.
Demir İ, Gürel N, Nalçacıoğlu R, Demirbağ Z (2009b). Comparative susceptibilities of six insect cell lines to infection by Malacosoma neustria nucleopolyhedrovirus (ManeNPV). Turk J Biol 33: 259273.
Demir İ, Nalçacıoğlu R, Demirbağ Z (2008). Te signifcance of insect viruses in biotechnology. Tarım Bilim Derg 14: 193201 (article in Turkish with an abstract in English).
Demir İ, Nalçacıoğlu R, Mohammad Gholizad L, Demirbağ Z (2013). Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey. Turk J Biol 37: 385391.
de Moraes RR, Maruniak JE (1997). Detection and identifcation of multiple baculoviruses using the polymerase chain reaction (PCR) and restriction endonuclease analysis. J Virol Methods 63: 209217.
Doğanlar M (1975). Erzurum bölgesinde önemli lepidopter tırtıllarında bulunan Tachinidae sinekleri ve bunların kısa biyolojileri. Atatürk Üniversitesi Yayınları, No. 375. Atatürk Üniversitesi Basımevi, Erzurum, 136 s. (article in Turkish).
Günther S (1958). Eine bisher unbekannte Mikrosporidie aus dem Ringelspinner (Malacosoma neustria L.: Lasiocampidae). Z Pf Krankh 65: 534535 (article in German).
Herniou EA, Olszewski JA, OReilly DR, Cory JS (2004). Ancient coevolution of baculoviruses and their insect hosts. J Virol 78: 32443251.
Ishii T, Nakai M, Okuna S, Takatsuka J, Kunimi Y (2003). Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and efects on larvae. J Invertebr Pathol 83: 206214.
Jankevica L, Cudare Z, Ose V (1998). New isolate of Malacosoma neustria nuclear polyhedrosis virus in Latvia. J Invertebr Pathol 71: 283285.
Jankevica L, Zarins I (1999). Virulance of Malacosoma neustria nucleopolyhedrovirus Latvian isolates. Latvijas Entomologs 37: 4045.
Keddie AB, Erlandson MA (1995). Characterization of a nuclear polyhedrosis virus from the forest tent caterpiller, Malacasoma disstria . J Invertebr Pathol 65: 4347.
Kikhno IM, Strokovskaya LI (1997). Physical mapping of Malacosoma neustria nuclear polyhedrosis virus genome. Biopolimery I Kletka 13: 218221 (article in Russian with an abstract in English).
Lacey LA, Frutos R, Kaya HK, Vail P (2001). Insect pathogens as biological control agents: do they have a future? Biol Control 21: 230 248.
Leathers TD, Gupta SC (1993). Susceptibility of the eastern tent caterpillar (Malacosoma americanum ) to the entomogenous fungus Beauveria bassiana . J Invertebr Pathol 61: 217219.
Lucarotti CJ, Morin B (1997). A nuclear polyhedrosis virus from the oblique banded leafroller, Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae). J Invertebr Pathol 70: 121126.
Magnoler A (1985). Field evaluation of baculovirus against Malacosoma neustria L. in Sardinia. La Difesa Delle Piante 2: 329338.
Moscardi F (1999). Assessment of the application of baculoviruses for control of lepidoptera. Annu Rev Entomol 44: 257289.
Muñoz D, Vlak JM, Cabellero P. (1997). In vivo recombination between two strains of the genus nucleopolyhedrovirus in its natural host, Spodoptera exigua . Appl Environ Microbiol 63: 30253031.
Murillo J, Muñoz D, Lipa JJ, Cabellero P ( 2001). Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae. J Appl Ent 125: 267270.
Nordin GL (1974). Pathogens of Malacosoma americanum in Kentucky (Lepidoptera: Lasiocampidae). J Kansas Entomol Soc 47: 249253.
Özbek H, Çalmaşur O (2005). A review of insects and mites associated with roses in Turkey. Acta Hortic 690: 167174.
Özbek H, Çoruh S (2010). Egg parasitoids of Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Erzurum province of Turkey. Turk Entomol Derg 34: 551560.
Progar RA, Rinella MJ, Fekedulegn D, Butler L (2010). Nuclear polyhedrosis virus as a biological control agent for Malacosoma americanum (Lepidoptera: Lasiocampidae). J Appl Entomol 134: 641646.
Reed C, Otvos IS, Reardon R, Ragenovich I, Williams HL (2003). Efects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1. J Invertebr Pathol 84: 104113.
Stene AE (1914). Two Common Caterpillars: Te Tent Caterpillar and the Fall Webworm. Rhode Island Board of Agriculture, EF Freeman Company.
Szewczyk B, Hoyos-Carvajal L, Paluszek M, Skrzecz I, Souza ML (2006). Baculoviruses reemerging biopesticides. Biotechnol Adv 24: 143160.
Teilmann DA, Blissard GW, Bonning B, Jehle JA, OReilly DR, Rohrmann GF, Tiem S, Vlak JM (2005). Baculoviridae. In: Fauquet CM, Mayo MA, Manilof J, Desselberger U, Ball LA, editors. Eighth Report of the International Committee on Taxonomy of Viruses. San Diego, CA, USA: Academic Press, pp. 177185.
Webb BA, Barney WE, Dalman DL, DeBorde SN, Weer C, Williams NM, Donahue JM, McDowell KJ (2004). Eastern tent caterpillars (Malacosoma americanum ) cause mare reproductive loss syndrome. J Insect Physiol 50: 185193.
Yearian WC, Young SY (1976). Preliminary evaluation of viral diseases of eastern tent caterpillar and fall webworm. Ark Farm Res 25: 3.