Effects of agar types on rooting performance in tissue culture: Sample of Quince A rootstock cultures

Çalışmada 0.5 gL−1 IBA veya NAA eklenen dört farklı ticari agar tipinin, Merck, Bacto, Oxoid, ve Gelrite, bilinen bir armut anacı olan Quince A’nın köklenmesi üzerine incelenmiştir. Çalışmada kullanılan agar tipine ve konsantrasyonuna bağlı olarak farklı köklenme oranları oluşmuştur. En yüksek köklenme oranı 9 gL−1 Oxoid agar içeren ortamda kaydedilmiştir. Kök sayısı ve uzunluğu Bacto agar eklenen ortamlarda en iyi olarak belirlenmiş, özellikle 7 gL−1 konsantrasyonu öne çıkmıştır. Gelrite Quince A’nın köklenmesi için uygun bulunmamıştır. İlk çalışmada, Merck (7 gL−1), Bacto (7 gL−1), ve Oxoid (7 & 9 gL−1) agar konsantrasyonları en iyi olarak belirlenmiş, çalışmanın ikinci kısmında bu agar konsantrasyonlarına 0.5 gL−1 IBA veya 0.5 gL−1 NAA ilave edilmiştir. IBA kök sayısı ve kök uzunluğu üzerinde daha etkili bulunmuştur. Ekonomiklik açısından değerlendirildiğinde Merck agar (7 gL−1) öne çıkarken, Bacto agar (7 gL−1) Quince A’nın kök kalitesi bakımından en iyi sonuçları vermiştir

Anahtar Kelimeler:

Agar tipleri, Quince A, in vitro köklenme, NAA, IBA, maliyet analizi

Effects of agar types on rooting performance in tissue culture: Sample of Quince A rootstock cultures

s This study investigated the effects of the four commercial gelling agents from Merck, Bacto, Oxoid, and Gelrite on in vitro rooting of, one of the popular pear rootstocks, Quince A. IBA or NAA, at 0.5 gL−1concentration, was added in the rooting media. Different rooting rates were observed according to used agar and auxin types. The highest rooting rate was recorded in the medium containing 9 gL−1 Oxoid agar. Root number and length were best in Bacto agar, especially at its 7 gL−1 concentration. Gelrite was unsuitable for rooting of Quince A. Optimum concentrations for Merck (7 gL−1), Bacto (7 gL−1), and Oxoid (7 & 9 gL−1) agar were selected in the first experiment, then they were supplemented with 0.5 gL−1 IBA or 0.5 gL−1 NAA in the second experiment. IBA was more effective on root number and root length than NAA. Merck agar (7 gL−1) was the most economical, Bacto agar (7 gL−1) was the most effective on root quality of Quince A

Keywords:

Agar types, Quince A, in vitro rooting, NAA, IBA, cost analysis,

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Afrasiab, H., Jafar, R., 2011. Effect of different media and solidifying agent on callogenesis and plant regeneration from different explants of rice (Oryza sativa L) varieties super basmati and IRRI-6. Pak J Biol Sci 43(1): 487-501. Anonymous, 2014a. Quince A. http://www.thompson-morgan.com/fruit- rootstock-guide
Campbell, J., 2003. Agfact H4.1.15, first edition, p:12.
Chauhan, J.K., Sharma, U., Sharma, S., 2012. Effect of rootstock and interstock on plant vigour of pear 2(1): Int J of Farm Sci 2(1): 29- 35.
Davarynejad, G., Shahriari, F., Hamid, H., 2008. Identification of graft incompatibility of pear cultivars on quince rootstock by using isozymes banding pattern and starch. Asian J of Plant Sci. 7(1):109-112.
Dumanoglu, H., Gulsen, Y., 1994. In vitro rooting of quince A (Cydonia oblonga Mill.). Acta Hort 367:360-363.
Ezekiel, A., 2010. Viable options and factors in consideration for low cost vegetative propagation of tropical trees. Int J Bot 6:187-193.
Gülşen, Y., Sülüşoğlu, M., Eroğlu, B., 1999. Quince A ayva anacının mikro üretiminde bazı agar tiplerinin kalitesine etkileri. III. Yumuşak Çekirdekli Meyveler Sempozyumu. Türkiye III. Ulusal Bahçe Bitkileri Kongresi Bildiri Kitabı, 17-21 Eylül, Ankara/Türkiye, 218-222. (abst. in English). ve
Henderson, J.M., 1987. The use of Gelrite as a substitute for agar in medium for plant tissue culture. Ala. Agric 2:5-6.
Henderson, W.E., Kinnersley, A.M., 1988. Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell Tissue Organ Cult 15:17-22.
Kumar-Chauhan, J., Sharma, U., Sharma, S0,. 2012. Effect of rootstock and interstock on plant vigour of pear. Int J of Farm Sci 2(1):29-35.
Lima, G.P.P., da Silva Campos, R.A., Willadino, L.G., Camara, Polyamines, gelling agents in tissue culture, micropropagation of medicinal Plants and Bioreactors. Recent Adv in Plant in vitro Culture 9:165-182 (ed: Annarita Leva and Laura M. R. Rinaldi, ISBN 978-953-51-0787- 3). F., 2012.
Mengesha, A., Ayenew, B., Gebremariam, E., Tadesse, T., 2012. Micro-propagation of Vanilla planifolia using enset (Ensete ventricosum (Welw, cheesman)) starch as a gelling agent. Current Research J of Bio Sci 4(4):519-525.
Mohamed, M.A.H., Alsadon, A.A., Al-Mohaidib, M.S., 2009. Corn and potato stach as an alternative micropropagation. Afr. J.Biotec 8:9199- 9203. tuberosum
Özel, Ç.A., Khawar, K.M., Arslan, O., 2008. A comparison of the gelling of isubgol, agar and Gelrite on in vitro shoot regeneration and rooting of variety Samsun of tobacco (Nicotiana tabacum L.). Scientia Hort 117:174-181.
Ruzic, Dj., Cerovic, R., 1998. Influence of agar brands and concentration on in vitro shoot multiplication of the cherry rootstock Gisela 5. Proc. Third. Int. Cherry Sym. Ed: Jonas Ystaas. Acta Hort 468:209-216.
Scholten, H.J., Pierik, R.L.M., 1998. Agar as a gelling agent:differential biological effects in vitro. Scientia Hort 77:109-116.
Sharma, S., Venkatasalam, E.P., Patial, R., Latawa, J., Singh, S., 2011. Influence of gelling agents and nodes on the growth of potato microplant. Potato J 38(1):41-46.
Stoltz, L.P., 1971. Agar restriction of the growth of excised mature Iris embryos. J Am Soc Hort Sci 96(5):618-684.
Welander, M., Maheswaran, G., 1992. Shoot regeneration from leaf explants of dwarfing apple rootstocks. J of Plant Physiol 140: 223- 228.
Zimmerman, R.H., Bhardwaj, S.V., Fordham, I.M., 1995. Use of starch-gelled medium for tissue of some fruit crops. Plant Cell Tiss Org Cult, 43: 207–213.