Demir, Selenyum ve Magnezyum Desteğinin Galleria mellonella Hücre Aracılı Bağışıklık Tepkileri Üzerindeki Etkileri

Bağışıklık tepkileri, tüm canlıların hayatta kalmasında önemli bir rol oynar. İnorganik materyallerin bünyeye alımı; canlı organizmalarda metabolizmaya ve bazı hücrelerin bileşenlerine katılımları nedeniyle kritik öneme sahiptir. Bu çalışmada demir (Fe), magnezyum (Mg) ve selenyum (Se) canlı organizmalardaki nükleik asitler ve enzimler gibi hücrelerin kritik bileşenlerine katılımları ve bağışıklık üzerindeki dolaylı ve doğrudan etkileri nedeniyle seçilmiştir. Bu inorganik maddelerin ayrı ayrı etkilerinin biliniyor olmasına rağmen, bunların birleşik etkileri üzerine bir çalışma bulunmamaktadır. Model organizma olarak kullanılan Galleria mellonella, çeşitli özelliklerinden dolayı insan patojenlerinin etkilerinin değerlendirilmesinde sıklıkla kullanılmaktadır. Çalışmada toplam hemosit sayımları, kapsülleme-melanizasyon bağışıklık tepkileri, incelenmiş olup G. mellonella'nın besin içeriğine % 0.1, % 0.25, % 0.5 ve % 1 oranında eklenen seçilmiş inorganik maddelerin, bu organizmanın immün yanıtları üzerindeki etkileri araştırılmıştır. Çalışma sonucunda kontrol grubuna göre dozların sadece % 0.25'inin hemosit sayısını artırdığı, diğer dozlarla ise önemli ölçüde azaldığı tespit edilmiştir. Bununla birlikte, % 0.5 ve % 1 oranları, hem hemosit sayısında hem de kapsülleme-melanizasyon yanıtlarında önemli bir düşüşe neden olmuştur. Bulgularımıza göre inorganik maddeler tarafından belirli bir dozun üzerinde bulunan besinler bağışıklık üzerinde olumsuz etkilere sahiptir.

Effects of Supplementation with Iron, Selenium and Magnesium on Galleria mellonella Cell-Mediated Immune Responses

Immune responses play a key role in the survival of all living things. Intake of inorganic materials is ofcritical importance in living organisms because of their participation in metabolism and some component ofcells. Iron (Fe), magnesium (Mg) and selenium (Se) were selected for this study because of their participation incritical constituents of cells, such as nucleic acids and enzymes in living organisms, and their indirect and directeffects on immunity. Although the separate effects of these inorganic materials are known, there is no study ontheir combined effects. Galleria mellonella, which is used as a model organism, is frequently used in evaluatingof effects of human pathogens due to its various properties. Total hemocyte counts and encapsulationmelanization responses are the most commonly observed parameters when examining the immune responsesof this model organism. In our study, the effects of the selected inorganic materials added in the ratio of 0.1%,0.25%, 0.5% and 1% in the nutrient content of G. mellonella on the immune responses of this organism wereinvestigated. As a result of the study, it was found that only 0.25% of the doses increased the hemocyte countand that the other doses significantly decreased compared to the control group. However, it was determinedthat 0.5% and 1% feeding caused a significant decrease in both hemocyte count and encapsulationmelanization responses. According to our findings, the nutrients which have over a certain dose by inorganicsubstances have negative effects on immunity.

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