Ramazan UZEN, Kemal GÜVEN, Medeni AYKUT, İbrahim Halil YILDIRIM, Fatma MATPAN BEKLER

Bingöl ve Diyarbakır İllerinde Yayılış Gösteren Bazı Dalgıç Böcek (Coleoptera: Dytiscidae) Türlerinin Mitokondrial Sitokrom Oksidaz Alt Ünite 1 Geni (COI) ile Filogenetik Analizi

Bu çalışmada, Bingöl ve Diyarbakır illerinden Eylül 2016 ile Mayıs 2017 dönemlerinde toplanan Dytiscidae familyasının 7 cinsine ait 17 türün PCR yöntemiyle mitokondriyal DNA örnekleri elde edilerek moleküler düzeyde araştırılmıştır. Bu türlerin mitokondriyal COI genlerinin nükleotit sekansları ve PCR genomik dizileri moleküler belirteç olarak kullanıldı. Mitokondriyal DNA dizi analizleri BLAST taraması yoluyla yapıldı. 17 türe ait mitokondriyal COI gen dizileri, türlerin tanımlamasında kullanılan CLC Sequence Viewer 8. programı yardımı ile filogenetik soy ağacı oluşturularak benzerlikleri karşılaştırıldı. Türlerin yakınlık dereceleri, Neighbour Joining (NJ) soy ağacı kullanılarak belirlenmeye çalışıldı. Analiz sonuçlarına göre; Agabus faldermanni (Zaitzev, 1927) sisteme kayıtlı kayıtlı nükleotid dizileriyle %100 örtüşürken, bu değer; Hydroporus planus (Fabricius, 1782)’ ta %99.88, Agabus biguttatus (Olivier, 1795) ve Laccophilus minutus (Linnaeus, 1758)’ta %99.86, Agabus glacialis Hochhuth, 1846’ da %99.74, Laccophilus poecilus Klug, 1834’ te %99.46, Agabus bipustulatus (Linnaeus, 1767)’ta %99.45, Agabus nebulosus (Forster, 1771)’ta %99.21, Hydroporus discretus Fairmaire and Brisout, 1859 ve Ilybius chalconatus (Panzer, 1796)’ ta %99.18, Agabus conspersus (Marsham, 1802)’ta %99.02, Bidessus calabricus Guignot, 1957’ ta %98.94, Nebrioporus stearinus suavis (Sharp, 1882)’ te %98.79, Hydroporus tessellatus (Drapiez, 1819)’ ta %97.45, Liopterus haemorrhoidalis (Fabricius, 1787)’ te %97.23, Hydroglyphus geminus (Fabricius, 1792)’te %96.47 ve Hydroporus palustris (Linnaeus, 1761)’ te %93.54 oranlarında benzerlikler belirlenmiştir.

Anahtar Kelimeler:

Bingöl, Diyarbakır, Dytiscidae, COI, Moleküler Tanımlama

PDF

___

Aykut, M. ve Fery, H. 2017. Scarodytes costatus nov. sp. from the Bingöl Province in Turkey, the first species of the genus with costate elytra (Insecta: Coleoptera: Dytiscidae: Deronectina). Linzer biologische Beiträge, 49: 395-414.
Aykut, M. ve Taşar, G.E. 2018. Contributions to the knowledge of Adephagan fauna in Adıyaman Province, Turkey (Coleoptera: Dytiscidae, Gyrinidae, Haliplidae and Noteridae). Munis Entomology&Zoology, 13: 249-255.
Aykut, M., Taşar, G.E. ve Fery, H. 2018. Deronectes taron sp.n. from the eastern Anatolian region of Turkey (Coleoptera, Dytriscidae, Hydroporinae). Zootaxa, 4422 (2): 403-410.
Aykut, M. 2018. The Diving Beetle Fauna of Diyarbakır and Bingöl Provinces, Turkey (Coleoptera: Dytiscidae) with a New Record. Pakistan Journal of Zoology, 50 (1): 65-74.
Aykut, M., Yıldırım, İ.H., Tusun, S. ve Fery, H. 2019. Deronectes kabilcevz sp. n. And D. Propedoriae sp. n. From South-eastern Anatolia (Turkey) (Coleoptera, Dytiscidae, Hydroporinae). Zootaxa, 4691 (1): 5-10.
Balke, M., Ribera, I. ve Vogler, A. P. 2004. MtDNA phylogeny and biogeography of Copelatinae, a highly diverse group of tropical diving beetles (Dytiscidae). Molecular Phylogenetics and Evolution, 32: 866–880.
Balke M., Pons J., Ribera I., Sagata K. ve Vogler A.P. 2007, Infrequent and unidirectional colonization of hyperdiverse Papuadytes diving beetles in New Caledonia and New Guinea. Molecular Phylogenetics and Evolution 42: 505–516.
Darılmaz, M.C. ve Kıyak, S. 2009. Checklist of Gyrinidae, Haliplidae, Noteridae and Dytiscidae of Turkey (Coleoptera: Adephaga). Journal of Natural History,43: 1585-1636.
Erman, Ö.K. ve Fery, H. 2000. Hydroporus erzurumensis sp.n. (Insecta: Coleoptera: Dytiscidae) from north-eastern Turkey. Annalen des Naturhistorischen Museums in Wien, 102B:171–176.
Erman, Ö.K. ve Erman, O. 2002. First records of Oreodytes Seidlitz, 1887 (Dytiscidae, Coleoptera) from Turkey: Oreodytes septentrionalis (Gyllenhal, 1826) and Oreodytes davisii (Curtis, 1831). Türkish Journal of Zoology, 26: 295-299.
Erman, Ö.K. ve Fery, H. 2006. Hydroporus neclae sp.n. from north-eastern Turkey, a new member of the memnonius-group of Hydroporus Clairville (Coleoptera: Dytiscidae). Zootaxa, 1355: 39–47.
Erman, Ö.K., Taşar, G.E., Aykut, M. ve Kurt, K. 2018. First record oh Hydaticus histrio Clarck, 1864 (Coleoptera, Dytiscidae) from Turkey. Acta Biologica Turcica, 31 (4): 174-177.
Ertorun, N. Şen, C. Ve Biter, E. 2014. Bazı Hydraenidae (Insecta: Coleoptera) Türlerinin Moleküler ve Morfolojik Analizleri. 22. Ulusal Biyoloji Kongresi, 23-27 Haziran, Eskişehir, s. 1141.
Fery, H. ve Erman, O.K. 2009. Five new species of the longulus-group of Hydroporus Clairville, 1806 from north-eastern Turkey (Coleoptera: Dytiscidae). Zootaxa, 2033: 1-12.
Fery, H. 2009. New species of the Hydroporus longulusgroup from Iran, Armenia and Turkey with a synopsis of the group (Coleoptera: Dytiscidae). Acta Entomologica Musei NationalisPragae,49: 529-558.
Fery, H. ve Hendrich, L. 2011a. Hydroporus esersi sp. n., a new diving beetle from southern Turkey (Coleoptera, Dytiscidae, Hydroporinae). Zootaxa, 2909: 38-46.
Fery, H. ve Hendrich, L. 2011b. Ilybius enpalaiatheka spec. nov. from Anatolia, Turkey, with a revised key to males of the Ilybius erichsoni and chalconatus groups. Spixiana, 34: 39-46
Fery, H. ve Przewoźny, M. 2011. Ilybius thynias sp. n. from European Turkey (Coleoptera: Dytiscidae). Zootaxa, 2740: 59-67.
Gustafson, GT. ve Miller, K.B. 2017. Systematics And Evolution Of The Whirligig Beetle Tribe Dineutini (Coleoptera: Gyrinidae: Gyrininae), Zoological Journal of the Linnean Society, XX: 1–33
Güz, N., ve Kılınçer, N. 2012. Böcek Sistematiğinde Moleküler Markörlerin Kullanımı. Türkiye Entomoloji Bülteni, 2 (2): 125-145.
Hájek, J., Šťastný, J., Boukal, M. ve Fery, H. 2011. Updating the eastern Mediterranean Deronectes (Coleoptera: Dytiscidae) with the description of two new species from Turkey. Acta Entomologica Musei NationalisPragae, 51: 463-476.
Hernando, C., Aguilera, P., Castro, A. ve Ribera, I. 2012. A new interstitial species of the Hydroporus ferrugineus group from north-western Turkey, with a molecular phylogeny of the H. Memnonius and related groups (Coleoptera: Dytiscidae: Hydroporinae). Zootaxa, 3173: 37-53.
Inoda, T. ve Balke, M. 2012. Status of Japanese Dytiscus Species (Coleoptera: Dytiscidae) Based On Mitochondrial DNA Sequence Data. Entomological Science, 15: 246–249.
Miller, K. B., Alarie, Y., William Wolfe, G. ve Whiting, M. F. 2005. Association of insect life stages using DNA sequences: the larvae of Philodytes umbrinus (Motschulsky) (Coleoptera: Dytiscidae). Systematic Entomology, 30: 499–509.
Miller, K. B. Jean, A., Alarie, Y., Hardy, N. ve Gibson, R. 2013. Phylogenetic placement of North American subterranean diving beetles (Insecta: Coleoptera: Dytiscidae). Arthropod Systematıcs & Phylogeny, 71 (2): 75-90.
Nilsson, A.N. ve Hájek, J. 2021a. Catalogue of Palearctic Dytiscidae (Coleoptera). World Wide Web Electronic Publication, http://www. waterbeetles.eu [accessed 25 February, 2021].
Nilsson, A.N. ve Hájek, J. 2021b. A world catalogue of the family Dytiscidae (Coleoptera, Adephaga). World Wide Web Electronic Publication, www.waterbeetles.eu [accessed 25 February, 2021].
Ribera, I., Hogan, J.E. ve Vogler, A. P. 2003. Phylogeny of Hydradephagan Water Beetles Inferred from 18S rRNA Sequences. Molecular Phylogenetics and Evolution, 23 (1): 43-62.
Ribera, I., Nilson, A.N. ve Vogler AP. 2004. Phylogeny and historical biogeography of Agabinae diving beetles (Coleoptera) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 30: 545–562.
Ribera, I., Vogler, A.P. ve Balke, M. 2007. Phylogeny and diversification of diving beetles (Coleoptera:Dytiscidae). Cladistics, 24: 563–590.
Trizzino, M., Jach, M.A., Audisio, P., Alonso, R. ve Ribera, I. 2013. A molecular phylogeny of the cosmopolitan hyperdiverse genus Hydraena Kugelann (Coleoptera, Hydraenidae). Systematic Entomology, 38: 192–208.
Yeşiloğlu, M. 2014. Hatay Yöresindeki Ochtheiıus Türlerinin (Hydraenidae; Coleoptera) Çeşitliliğinin Mitokondriyal Cox Alt Ünite 1 Geni Hedeflenerek Ortaya Çıkarılması, (Yüksek Lisans Tezi), Mustafa Kemal Üniversitesi, Hatay, s. 41.