Alpaslan TERZİ, Sacit ÇOBAN, Fahrettin YILDIZ, Murat KAYA, Nurten AKSOY, ABDULLAH TAŞKIN

Oxidative status and plasma prolidase activity in patients with gallstones

Amaç: Bu çalışmadaki amacımız safra kesesi taşı olan hastalarda oksidatif ve antioksidatif durumu ve plazma prolidaz enzim aktivitesini araştırmaktır. Gereç ve Yöntemler: Çalışma grubu safra kesesi taşı olan 35 hastadan ve kontrol grubu sağlıklı 30 kişiden oluşturuldu. Plasma prolidaz aktivitesi ve oksidatif-antioksidatif parametre düzeyleri çalışıldı. Antioksidatif durum total antioksidan kapasite ölçülerek değerlendirildi. Oksidatif stresi değerlendirmek için total oksidan durum ve oxidatif stres indeksi çalışıldı. Bulgular: Plazmada total antioksidan kapasite düzeyi safra kesesi taşı olan hastalarda kontrol grubuna göre daha düşüktü (p

Safra kesesi taşı olan hastalarda oksidan durum ve plazma prolidaz aktivitesi

Objective: The aim of our study was to investigate the oxidative and antioxidative status of plasma and plasma prolidase enzyme activity on gallstone disease. Material and Methods: The study group included 35 patient with gallstone disease and 30 healthy volunteers as control subjects. Plasma prolidase activity and oxidative-antioxidative parameters were measured. Antioxidative status of plasma was evaluated by measuring total antioxidant capacity. To determine oxidative status; total oxidant status and oxidative stress index were deermied. Results: Total antioxidant capacity levels of plasma were lower in the patients with gallstone disease than in controls (p<0.001). In contrast, total oxidant status levels and oxidative stress index values were higher in the patients than in controls (p<0.05 and p<0.001, respectively). Beside these, plasma prolidase activity was significantly lower in the patients than controls (p<0.001). Conclusions: The present results indicated that the oxidant parameters increased and antioxidant parameters decreased in patients with gallstones, and that these patients were exposed to oxidative stress.

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1) Weltman DI, Zeman RK. Acute diseases of the gallbladder and biliary ducts. Radiol Clin North Am 1994;32:933-50.
2) Baig SJ, Biswas S, Das S, Basu K, Chattopadhyay G. Histopathological changes in gallbladder mucosa in cholelithiasis: Correlation with chemical composition of gallstones. Trop Gastroenterol 2002;23:25-7.
3) Seki S, Kitada T, Yamada T, Sakaguchi H, Nakatani K, Onoda N, et al. Immunohistochemical detection of 8- hydroxydeoxyguanosine, a marker of oxidative DNA damage, in human chronic cholecystitis. Histopathology 2002;40:531-5.
4) Jüngst C, Sreejayan, Eder M. I, von Stillfried N, Zündt B, Spelsberg F. W, Kullak-Ublick G. A, Jüngst D, von Ritter C. Lipid peroxidation and mucin secretagogue activity in bile of gallstone patients. European Journal of Clinical Investigation 2007;37:731-6.
5) Geetha A. Evidence for oxidative stress in the gall bladder mucosa of gallstone patients. J Biochem Mol Biol Biophys. 2002;6:427-32.
6) Matsui A, Ikeda T, Enomoto K, Hosoda K, Nakashima H, Omae K, et al. Increased formation of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 and COMT genotypes. Cancer Lett. 2000;151:87-95.
7) Galli F, Piroddi M, Annetti C, Aisa C, Floridi E, Floridi A. Oxidative stress and reactive oxygen species. Contrib Nephrol 2005;149:240-60.
8) Aslan M, Nazligul Y, Horoz M, Bolukbas C, Bolukbas FF, Aksoy N, et al. Serum prolidase activity and oxidative status in Helicobacter pylori infection. Clin Biochem. 2007;40:37-40.
9) Kavalcı C, Durukan P, İlhan N, Güzel A, Ömürlü Kİ. Kafa ici yaralanmanın tanısında serum MDA'nın yeri. Trakya Univ Tıp Fak Derg. 2008;25:209-13.
10) Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994;344:721-4.
11) Aruoma OI Characterization of drugs as antioxidant prophylactics. Free Radic Biol Med 1996;20:675-705.
12) Sontakke AN, Tare RS. A duality in the roles of reactive species with respect to bone metabolism. Clin Chim Acta 2002;318:145-14.
13) Palka JA, Phang JM. Prolidase activity in fibroblasts is regulated by interaction of extracellular matrix with cell surface integrin receptors. J Cell Biochem 1997;67:166-75.
14) Myara I, Myara A, Mangeot M, Charpentier C, Lemonnier A. Plasma prolidase activity: a possible index of collagen catabolism in chronic liver disease. Clin Chem 1984;30:211-5.
15) Erbagci AB, Araz M, Erbagci A, Tarakcioglu M, Namiduru ES. Serum prolidase activity as a marker of osteoporosis in type 2 diabetes mellitus. Clin Biochem 2002;35:263-8.
16) Altindag O, Erel O, Aksoy N, Selek S, Celik H, Karaoglanoglu M. Increased oxidative stress and its relation with collagen metabolism in knee osteoarthritis. Rheumatol Int 2007;27:339-44.
17) Gejyo F, Kishore BK, Arakawa M. Prolidase and prolinase activities in the erythrocytes of patients with chronic uremia. Nephron 1983;35:58-61.
18) Ozcan O, Gultepe M, Ipcioglu OM, Bolat B, Kayadibi H . Optimization of the Photometric Enzyme Activity Assay For Evaluating Real Activity of Prolidase. Turk J Biochem 2007; 32:12-6.
19) Myara I, Cosson C, Moatti N, Lemonnier A. Human kidney prolidase--purification, preincubation properties and immunological reactivity. Int J Biochem. 1994;26:207-14.
20) Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37: 112-9.
21) Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38:1103-11.
22) Bolukbas C, Bolukbas FF, Horoz M, Aslan M, Celik H, Erel O . Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis 2005;5:95.
23) Sipos P, Krisztina H, Blázovics A, Fehér J. Cholecystitis, gallstones and free radical reactions in human gallbladder. Med Sci Monit. 2001;7:84-8.
24) Chamulitrat W, Carnal J, Reed NM, Spitzer JJ. In vivo endotoxin enhances biliary ethanol-dependent free radical generation. Am J Physiol, 1998;274: 653-61.
25) Nilsson B, Delbro D, Hedin L, Conradi N, Thune A, Friman S, et al. Role of nitric oxide induction of inflammatory fluid secretion by the mucosa of the feline gallbladder. Gastroenterology, 1996;110: 598-606.
26) Schottenfeld D, Beebe-Dimmer J. Chronic inflammation: a common and important factor in the pathogenesis of neoplasia. CA Cancer J Clin. 2006;56:69-83.
27) Kundak AA, Erbas H, Gülen S, Dökmeci G, Celik H, Özcan T. C ve E vitaminlerinin, kronik olarak alkolle beslenen sıçanlarda beyin dokusu arjinaz aktivitesi, ornitin ve üre düzeylerine etkileri. Trakya Univ Tıp Fak Derg. 2007;24:49-55.