Organophosphates (OPs) and other pesticides are used to eradicate agricultural pests. Gardeners are the most risky group prone to side effects of pesticides. Organophosphates (OPs) inhibit acetyl cholinesterase (AChE) enzyme and as a result excess nicotinic and muscarinic neurostimulation develops. Chronic neurotoxic effects may be irreversible. For evaluation of pesticide exposure in human body specific biomarkers that are determined by authors are needed. We evaluated gardeners laboratory test results retrospectively. The purpose of this study was to determine the biomarkers of occupational pesticide exposure in gardeners.