Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates

Öz The immobilization of horseradish peroxidase in the form of cross-linked enzyme aggregates (HRP–CLEAs) was optimized by three parameters full factorial experimental design at two levels and the degradation of an azo dye was tested. The optimal immobilization conditions were estimated as 0.06 mg enzyme/mL (0.96 U), 3 % (v/v, Glutaraldehyde)) cross-linker ratio, and 6 mg /mL Bovine Serume Albumine amount, respectively. The effects of variables were analysed by responce surface plots and the enzyme concentration effect was insignificant for the tested levels. The maximum CLEAs activity was 0.2188 U and the kinetic parameters (Km and Vmax) were 0.0314 mM and 0.1044 mM/min while the calculated values for soluble enzyme were 0.06 mM and 0.468 mM/min, respectively. Hydrogen peroxide has inhibitory effect on enzyme aggregate activities at higher amounts than 0.012 mM. Under optimal conditions, a textile azo dye, Reactive Blue 160 was completely oxidized within 90 h and higher temperatures also showed promising results in dye degradation.  

Kaynakça

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Kaynak Göster

Bibtex @araştırma makalesi { tekstilvekonfeksiyon498774, journal = {Textile and Apparel}, issn = {1300-3356}, eissn = {2602-3075}, address = {Ege Üniversitesi Tekstil ve Konfeksiyon Araştırma Uygulama Merkezi 35100 Bornova - İZMİR}, publisher = {Ege Üniversitesi}, year = {2021}, volume = {31}, pages = {34 - 42}, doi = {10.32710/tekstilvekonfeksiyon.498774}, title = {Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates}, key = {cite}, author = {Ayhan, Fatma and Akpolat, Oguz} }
APA Ayhan, F , Akpolat, O . (2021). Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates . Textile and Apparel , 31 (1) , 34-42 . DOI: 10.32710/tekstilvekonfeksiyon.498774
MLA Ayhan, F , Akpolat, O . "Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates" . Textile and Apparel 31 (2021 ): 34-42 <
Chicago Ayhan, F , Akpolat, O . "Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates". Textile and Apparel 31 (2021 ): 34-42
RIS TY - JOUR T1 - Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates AU - Fatma Ayhan , Oguz Akpolat Y1 - 2021 PY - 2021 N1 - doi: 10.32710/tekstilvekonfeksiyon.498774 DO - 10.32710/tekstilvekonfeksiyon.498774 T2 - Textile and Apparel JF - Journal JO - JOR SP - 34 EP - 42 VL - 31 IS - 1 SN - 1300-3356-2602-3075 M3 - doi: 10.32710/tekstilvekonfeksiyon.498774 UR - Y2 - 2021 ER -
EndNote %0 Tekstil ve Konfeksiyon Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates %A Fatma Ayhan , Oguz Akpolat %T Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates %D 2021 %J Textile and Apparel %P 1300-3356-2602-3075 %V 31 %N 1 %R doi: 10.32710/tekstilvekonfeksiyon.498774 %U 10.32710/tekstilvekonfeksiyon.498774
ISNAD Ayhan, Fatma , Akpolat, Oguz . "Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates". Textile and Apparel 31 / 1 (Mart 2021): 34-42 .
AMA Ayhan F , Akpolat O . Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates. Textile and Apparel. 2021; 31(1): 34-42.
Vancouver Ayhan F , Akpolat O . Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates. Textile and Apparel. 2021; 31(1): 34-42.
IEEE F. Ayhan ve O. Akpolat , "Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates", Textile and Apparel, c. 31, sayı. 1, ss. 34-42, Mar. 2021, doi:10.32710/tekstilvekonfeksiyon.498774