Özgür SAĞLAM, Ali Arda IŞIKBER, Hasan TUNAZ, M. Kubilay ER, Fatih BAHADIR, Recep ŞEN

Preliminary Checking of Some Turkish Diatomaceous Earth Similarities with Commercial Diatomaceous Earths under Scanning Electron Microscope (SEM)

Diatoms are dead bodies of unicellular algae's and made up of fossilized diatoms in aquatic ecosystems. Diatomaceous earth (DE) is a dust varying in color depending on composition, from white-grey to yellow to red and active ingredient is amorphous silicon dioxide. DEs are commonly used for purification of water, the purification of juices, separation of various oils and chemicals and also used as an insecticide. Mode of action as insecticide which damage occurs to the insects protective wax coat on the cuticle, mostly by sorption and to a lesser degree by abrasion, or both. The result is the loss of water from the insect's body through desiccation resulting in death. The efficacy of DE against insects depends on different physical and morphological characteristics of the diatoms. In present study, image properties of 10 different Turkish DE samples under Scanning Electron Microscope (SEM) were checked and compared similarities with commercial DEs, namely Protector,SilicoSec, Insecto and Pyrisec. SEM image analysis indicated that there were variations in shape and size of dead bodies of diatoms in Turkish and commercial DEs. The shapes of dead bodies of diatoms in Turkish local DE's named as CBN and BGN were found very similar with those in commercial DE, Silicosec. Local DE coded as DC has a round shape and looks similar to commercial DE of Pyrisec while local DE coded as CAN has triangle shape and its shape was different from those of all other DE samples.

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Anonymous, 2017a. Diatomite Mining and Processing,http://diatomite.org/DiatomiteMining-and-Processing.IDPA, International diatomite producers association. (Accessed date: 05.05.2017).

Anonymous, 2017b. Scanning Electron Microscopy (SEM). https://en.wikipedia.org/wiki/Scanningelectro nmicroscope (Accessed date: 05.05.2017).

Athanassiou, C.G., B.J. Vayias, C.B. Dimizas, N.G. Kavallieratos, A.S. Papagregoriou and C.Th. Buchelos, 2005. Insecticidal efficacy of diatomaceous earth against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Tribolium confusum du Val (Coleoptera: Tenebrionidae) on stored wheat: influence of dose rate, temperature and exposure interval. Journal of Stored Products Research, : 41- 47.

Athanassiou, C.G., N.G. Kavallieratos, B.J. Vayias, Z. TomanoviC, A. Petrovic, V. Rozman, C. Adler, Z. Korunic and D. Milovanovic, 2011. Laboratory evaluation of diatomaceous earth deposits mined from several locations in central and southeastern Europe as potential protectants against coleopteran grain pests. Crop protection 30: 329-339.

Bhishma, R.S., S.H. Alavi, S.P. Harimkar, M. McCollum, J.F. Donoghue and F.D. Blum, 2017. Particle morphology dependent superhydrophobicity in treated diatomaceous earth/ polystyrene coatings, Applied Surface Science 416:947-956.

Calvert, R. 1930. Diatomaceous earth Journal of Chemical Education, 7: 28-29.

Çetin, M. and B. Taş, 2012. Biyolojik orjinli tek mineral: Diyatomit. Türk Bilim Araştırma Vakfı (TÜBAV) Bilim Dergisi, 5(2): 28-46.

Parkinson, J. and R. Gordon, 1999. Beyond micromachining: the potential of diatoms. Trends Biotechnology, 17:190-196.

Round, F., R. Crawford and D. Mann, 1990. The Diatoms: Biology and Morphology of the Genera, Cambridge University Press, UK.

Katz, H. 1991. Desiccants: dry as dust means insect's death. Pest Control Technology, 82-84.

Korunic, Z., P.G. Fields, M.I.P. Kovacs, J.S. Noll, O.M. Lukow, C.J. Demianyk and K.J. Shibley, 1996. The effect of diatomaceous earth on grain quality. Postharvest Biology and Technology, 9(3): 373-387.

Korunic, Z. 1997. Rapid assessment of the insecticidal value of diatomaceous earths without conducting bioassays. Journal of Stored Products Research, 33, 219-229.

Korunic, Z. 1998. Diatomaceous earths, a group of natural insecticides. Journal of Stored Products Research, 34: 87-97.

Doğanay, İ.Ş. , A.A. Işıkber, Ö. Sağlam and Y. Bilgili, 2014. Efficiency of some Turkish diatomaceous earth deposits against stored-grain insects, Sitophilus granarius (L.) and Rhyzopertha dominica (F.). International Conference on Biopesticide (ICOP 7), p.91.

Ertürk, S. 2014. Farklı diyatom toprağı formülasyonlarının depolanmış çeltikte zararlı böceklere etkinliği üzerinde araştırmalar. Ankara Üniversitesi, Fen bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Doktora Tezi, s.120.

FDA (Food and Drug Administration, USA), 1995. Specifications for diatomaceous earths as a maximum 2 % animal feed additive. 21 CFR Section, 573.340.

Işıkber, A.A., Ö. Sağlam, M.K. Er and H. Tunaz, 2016. Potential of Turkish diatomaceous earth formulations as natural grain protectants for control of stored grain insects. 15 th International Cereal and Bread Congress, p.42.

Işıkber, A.A., Ö. Sağlam, M.K. Er, H. Tunaz and R. Şen, 2015. Toxicity of Turkish diatomaceous earth deposits against some stored-grain insects on wheat. 10th Conference of IOBC/WPRS Working Group "Integrated Protection of Stored Products" Congress, p.29.

McLaughlin, A. 1994. Laboratory trials on desiccant dust insecticides. 6th InternationalWorking Conference on Stored-Product Protection. CAB, Wallingford, Canberra, Australia, pp. 638-645.

Mete, Z. 1998. Kütahya-Alayunt Yöresi Diyatomit Yataklarının Zenginleştirilmesi, Akdeniz Üniversitesi Isparta. Mühendislik Fakültesi Dergisi, 184-201.

Özbey, G. and N. Atamer, 1987. Kizelgur (Diatomit) hakkında bazı bilgiler. 10. Türkiye Madencilik Bilimsel Teknik Kongresi, Ankara, s.493-502.

Round, F., R. Crawford and D. Mann, 1990. The Diatoms: Biology and Morphology of the Genera, Cambridge University Press, UK.

Sıvacı, R. and Ş. Dere, 2006. Melendiz Çayı'nın (Aksaray-Ihlara) Epipelik Diyatome Florasının Mevsimsel Değişimi. C.Ü. Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 27(1): 1-12.

Snetsinger, R. 1988. Report on Shellshock insecticide. Report of Department of Entomology, Pennsylvania State University, pp. 1-7.

Subramanyam, B. and R. Roesli, 2000. Inert dust. In Subramanyam Bh, Hagstrum, D.W. (Eds), Alternatives to Pesticides in Stored - Product IPM. Kluwer Academic Publishers, Boston, MA: p.321-379.