Ebrahim SABBAGH, Seyed Kazem SABBAGH, Naser PANJEHKEH, Hamid Reza BOLOK-YAZDI

Pythium aphanidermatum ile Bulaşık Hıyarlarda Jasmonik Asitin Uyardığı Sistemik Dayanıklılık

Çökerten hastalığı dünyanın her yerinde hıyarlarda en tahripkâr hastalıklarından biridir. Bu çalışmada, çökerten hastalığına karşı jasmonik asitin (JA) uyarılmış dayanıklılığı teşvik etme potansiyeli incelenmiştir. JA’nın polifenoloksidaz (PPO),peroxidaz (PO) ve katalaz (CAT) enzimlerinin aktiviteleri ve toplam fenollere etkisi spektrofotometrik yöntemle belirlenmiştir. Üç bitki savunma geninin, Lipoxygenase, Cupi4 ve Phenylalanine ammonia-lyase ifade seviyeleri qRTPCR yöntemi kullanılarak analiz edilmiştir. Mantarın dairevi gelişimine JA’nın engelleyici etkisi damlatma yöntemi kullanılarak tespit edilmiştir. JA’nın dıştan uygulanması bulaşık bitkilerdeki hastalık şiddetini azaltmasına rağmen katı ortamda misel gelişimini engellememiştir. Sonuçlarımız, farklı yoğunluklarda JA uygulamasının oksidatif enzim faaliyetini büyük oranda artırdığını göstermiştir. En yüksek enzim faaliyeti 400 mg L-1 JA yoğunluğunda bulaştırmadan 48 saat sonra kaydedilmiştir. Gen ifadesi analizleri bulaştırmadan 48 saat sonra JA’nın bütün test edilen genlerin mRNA kopyalarını farklı da olsa artırabildiğini ortaya koymuştur. Cupi4 geninin ifade edilme seviyesi, uygulama yapılan bitkilerde, diğer incelenen genlerinkinden daha fazla bulunmuştur. JA tarafından uyarılmış sistemik dayanıklılık, ISR işaretleyici genlerinin ifade edilmesi ve antioksidan enzim faaliyetinin artışı vasıtasıyla olmuştur. Sonuçlar, JA’nın dıştan uygulanmasının bitki dayanıklılığını uyarıcı olarak kabul edilebileceğini göstermiştir.

Jasmonic Acid Induced Systemic Resistance in Infected Cucumber by Pythium aphanidermatum

Damping-off disease of cucumber is one of the most destructive diseases of cucumber in worldwide. In this work, the potential of jasmonic acid (JA) for induce resistant against damping off disease was investigated. The effect of JA on activity of Polyphenol oxidase (PPO), Peroxidase (PO) and Catalase (CAT) enzymes and total phenol was assayed by spectrophotometric method. Expression level of three plant defense genes as Lipoxygenase, Cupi4 and Phenylalanine ammonia-lyase genes was analyzed using qRT-PCR method. Drop-plate method was used to assay inhibitory effect of JA on radial growth of fungi. Exogenic application of JA decreased disease severity in the infected plants but did not inhibit mycelia growth on solid medium compared to control. Our results showed that JA application substantially increased the activity of oxidative enzymes at different concentration. The highest enzyme activity was recorded after 48 hours post infection (hpi) at a concentration of 400 mg L-1 of JA. Gene expression analysis revealed that JA is differentially able to increase the mRNA transcripts of all tested genes at 48 hpi. The expression level of Cupi4 gene was higher than the other genes in treated plants. Induced systemic resistance by JA was mediated through an enhanced expression of ISR marker genes and increase of antioxidant enzymes activity. Based on these results, we suggest that exogenic application of JA could be considered as plant resistance inducer.

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