Genetic analysis of maize (zea mays l.) hybrids using microsatellite markers

Genetik safık, hibrit tohum üretimi için gerekli olan önemli kalite kriterlerinden biridir. Bu çalışmada, molekülerişaretleyiciler ticari olarak önemli 3 mısır F 1 hibrit çeşidi (Pasha, Frida ve PG1661) ve kendilenmiş saf ebeveyn hatlarınıngenetik safık ve çeşitlilik analizleri için kullanılmıştır. Örnekler, elli adet basit dizi tekrarları (SSR) işaretleyicilerikullanılarak analiz edilmiş ve işaretleyicilerin verimliliği karşılaştırılmıştır. Testlenen 50 işaretleyici içinden 23 primerçifti ortalama 0.69 polimorfzm bilgisi değeri ile değişik hibrit çeşitleri arasındaki farklılığı tespit etmiştir. Hibrittohumların genetik safık analizi, % 98den yüksek homoloji oranı ile sonuçlanmıştır. Hibritler, 3 ana gruba toplamıştır.Bu çalışma ile moleküler işaretleyicilerin mısır hibritlerinin genetik safık ve çeşitlilik analizleri için kullanılmasınınverimli olduğu ve mikrosatellitlerin ko-dominantlık özellikleri nedeni ile doğru işaretleyiciler olduğu saptanmıştır.

Mısır (zea mays l.) hibritlerinin mikrosatellit işaretleyiciler kullanılarak genetik analizleri

Genetic purity is one of the most important quality criteria required for successful hybrid seed production. In this study,molecular markers were used for assessing the genetic purity and diversity of three commercially important maize F 1hybrids (Pasha, Frida and PG1661) and their parental inbred lines. Fifty Simple Sequence Repeats (SSRs) markers wereused to analyze samples, also the effciency of the markers were compared. Twenty three primer pairs among the fftymarkers were able to detect polymorphism between the different types of hybrids with an average of 0.69 polymorphisminformation content (PIC) value. Genetic purity analyses revealed more than 98% homogeneity in the hybrid seeds. Thehybrids were grouped into three main clusters. It can be concluded that, molecular markers are effcient to study the geneticpurity and diversity in maize hybrids and microsatellites are more accurate marker-type because of their co-dominancy.

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Ajmone-Marsan P, Castiglioni P, Fusari F, Kuiper M & Motto M (1998). Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers. Theoretical and Applied Genetics 96 : 219-227
Anderson J A, Churchill G A & Autrique J E (1993). Optimizing parental selection for genetic linkage maps. Genome 36 : 181-186
Bornet B & Branchard M (2001). Nonanchored inter simple sequence repeat (ISSR) markers: Reproducible and specifc tools for genome fngerprinting. Plant Molecular Biology Reporter 19 : 209-215
Dangle G A, Mendum M L, Prins B H, Walker M A, Meredith C P & Simon C J (2001). Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genomet 44 : 432-438
Daniel I O, Adetumbi J A, Oyelakin O O, Olakojo S A, Ajala M O & Onagbesan S O (2012). Application of SSR markers for genetic purity analysis of parental inbred lines and some commercial hybrid maize (Zea mays L.). American Journal of Experimental Agriculture 2 (4): 597-606
Doyle J J & Doyle J L (1990). Isolation of plant DNA from fresh tissue. Focus 12 : 13-15
Dubreuil P & Charcosset A (1998). Genetic diversity within and among maize populations: A comparison between isozyme and nuclear RFLP loci. Theoretical and Applied Genetics 96 : 577-587
FAO (2012). Statistical database. Available at: http:// www.fao.org
Gül İnce A, Karaca M & Onus A N (2011). Exact microsatellite density differences among capsicum tissues and development stages. Tarım Bilimleri Dergisi-Journal of Agricultural Sciences 17 (4): 291- 299
Hipi A, Surahman M, Ilyas S & Giyanto (2013). Seed genetic purity assessment of maize hybrid using microsatellite markers (SSR). International Journal of Applied Science and Technology 3 (5): 66-71
Koranyi P (1989). Simple purity checking of maize lines and hybrids by protein monomer analysis. Seed Science and Technology 17 : 161-168
Lucchese C, Dinelli G, Miggiano A & Lovato (1999). Identifcation of pepper (Capsicum spp) cultivars by feld and electrophoresis tests. Seed Science and Technology 27 : 37-47
MaizeGDB (2013). Maize Genetics and Genomics Database. Available at: http:// http://www.maizegdb. Org
Mammadov J A, Chen W, Ren R, Pai R, Marchione W, Yalçın F, Witsenboer H, Greene T W, Thompson S A & Kumpatla S P (2010). Development of highly polymorphic SNP markers from the complexity reduced portion of maize [Zea mays L.] genome for use in marker-assisted breeding. Theoretical and Applied Genetics 121 (3): 577-88
Mingsheng W, Xihai J, Lei T & Baochun L (2006). Rapid and reliable purity identifcation of F1 hybrids of maize (Zea mays L.) using SSR markers. Molecular Plant Breeding 4 (3): 381-384
Pejic I, Ajmone-Marsan M, Morgante M, Kozumplick V, Castiglioni P, Taramino G & Motto M (1998). Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theoretical and Applied Genetics 97 : 1248-1255
Powell W, Morgante M, Andre C, Hanagey M, Vogel J, Tingey S & Rafalski A (1996). The comparison of RFLP, RAPD AFLP and SSR (microsatellite) markers for germplasm analysis.
Molecular Breeding 2 : 225- 238 Rohlf F J (2000). NTSYS-PC. Numerical Taxonomy and Multivariate Analysis System. Version 2.0 Exeter Software, Setaket, NewYork
Roos E E & Wianer L E (1991). Seed testing and quality assurance. HortTechnology 11 : 65-69
Salgado K C P C, Vieira M D G G C, Pinho E V R V, Guimaraes C T, Pinho R G V & Sousa R G V (2006). Genetic purity certifcate in seeds of hybrid maize using molecular markers. Revista Brasileira de Sementes 28 (1): 169-175
Shehata A I, Al-Ghethar H A & Al-Homaidan A A (2009). Application of simple sequence repeat (SSR) markers for molecular diversity and heterozygosity analysis in maize inbred lines. Saudi Journal of Biological Sciences 16 : 57-62
Srdic J, Mladenovic Drinic S, Pajic Z & Filipovic M (2007). Characterization of maize inbred lines based on molecular markers, heterosis and pedigree data. Genetika 39 (3): 355-363
Wang C, Bian K, Zhang H, Zhou Z & Wang J (1994). Polyacrylamide gel electrophoresis of salt soluble proteins for maize variety identifcation and genetic purity assessment. Seed Science and Technology 22 : 51-57
Warburton M L, Zianchun X, Crossa J, Frnaco J, Melchinger A E, Frisch M, Bohn M & Hoisington D (2002). Genetic characterization of CIMMYT inbred maize lines and open pollinated populations using large scale fngerprinting methods. Crop Science 42 : 1832-1840