BURAK KAPTANER, Güler ÜNAL

Apoptosis in postovulatory follicles of Chalcalburnus tarichi Pallas, 1811

İnci kefali Chalcalburnus tarichi Pallas, 1811 postovulasyon foliküllerinde apoptoz. Bu çalışmada, inci kefali (Chalcalburnus tarichi) postovulasyon foliküllerinde apoptoz, TUNEL boyama ve agaroz jel elektroforez metodları ile araştırıldı ve plazma 17β-estradiol (E2) seviyesi ovulasyondan sonra 1., 3. ve 5. günlerde belirlendi. Postovulasyon foliküllerinde apoptotik hücreler, histolojik kesitlerde, ovulasyondan sonra 1. günde işaretlendi ve apoptozun çoğunlukla granuloza nadiren de teka hücrelerinde meydana geldiği belirlendi. Apoptozun işareti olan oligonükleozomal büyüklükteki DNA parçalanması ovulasyondan sonra 1., 3. ve 5. günlerde gözlendi. Ovulasyon anında oligonükleozomal DNA parçalanması gözlenmezken, 3. ve 5. günlerde bu parçalanmanın belirgin olduğu görüldü. Plazma E2 seviyesi ovulasyondan sonra dereceli olarak düştü. Bu düşüş 3. ve 5. günlerde önemliydi (P

Anahtar Kelimeler:

folikül, ovulasyon, Türkiye, programlanmış hücre ölümü

İnci kefali Chalcalburnus tarichi Pallas, 1811 postovulasyon foliküllerinde apoptoz.

In the present study, apoptosis in postovulatory follicle was investigated with TUNEL staining and agarose gel electrophoresis methods, and plasma 17β-estradiol (E2) level was determined at the 1st, 3rd and 5th day after ovulation in Chalcalburnus tarichi. Apoptotic cells were labelled in postovulatory follicles in histological sections on the 1st day after ovulation and it is indicated that apoptosis was observed mainly in granulosa cells and scarcely in theca cells. Oligonucleosomal length DNA fragmentation which is hallmark of apoptosis was observed on the days 1, 3 and 5 after ovulation. While no oligonucleosomal DNA fragmentation was observed on the ovulation time, it was observed that this fragmentation was remarkable on the days 3 and 5. Plasma E2 level decreased gradually after ovulastion. This decrease was significant on the days 3 and 5 (P<0.05). These results demonstrate that apoptosis is responsible for elimination of postovulatory follicles however it was considered that low level of E2 after ovulation, accelerates the postovulatory follicle apoptosis in Chalcalburnus tarichi.

Keywords:

follicles, ovulation, Turkey, apoptosis,

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