Roles of Adiponectin Signaling Related Proteins in Mammary Tumor Development

Objective: This study aims to investigate the expression levels of adiponectin signaling related proteins in mammary tissue, liver and breast cancer tissue in mice. Adiponectin, an adipocytokine, is secreted from adipose tissue and has been documented to have roles in diabetes, inflammation, and cancer development. In particular, levels of serum adiponectin are inversely associated with obesity and a decrease in serum adiponectin levels have been reported to be associated with breast cancer. There are two adiponectin receptor subtypes, AdipoR1 and AdipoR2, which have been identified in mammalian tissues, including human cancer cell lines and also in human mammary tumors. However, the role of adiponectin receptors in breast cancer development remains to be established. Methods: In this study, MMTV-TGF-alpha transgenic mice were fed from week 10 up to week 74 of age. Expression levels of adiponectin, AdipoR1 and AdipoR2 proteins were measured in the mammary fat pad (MFP), mammary tumor (MT) and liver tissues from 74 weeks old MMTV-TGF-alpha transgenic mice with and without MT using Western Blot. Adiponectin levels were measured using ELISA assay. Results: Protein expression levels of Adiponectin and AdipoR1 were significantly lower in MTs compared to control tissues. However, AdipoR2 protein expression levels were similar in MT and MFP tissues from MT-positive and MT-negative mice. The expression levels of adiponectin, AdipoR1 and AdipoR2 proteins in liver tissues were also similar in MT-positive and MT-negative mice. Serum adiponectin levels of the MT-positive and MT-negative mice were similar. Conclusion: These results indicate that adiponectin and its receptors are differentially regulated depending upon the specific tissue analyzed. AdipoR1 and adiponectin may play important roles in MT development.

Meme Tümörü Gelişiminde Adiponektin Sinyal Yolağı Proteinlerinin Rolü

Amaç: Bu çalışmanın amacı fare karaciğer, meme dokusu ve meme tümörü dokularındaki adiponektin sinyal yolağı ile ilişkili proteinlerin ekspresyon seviyelerinin belirlenmesidir. Adiponektin reseptörünün, AdipoR1 ve AdipoR2 olmak üzere memeli dokusunda belirlenmiş iki alt tipi vardır. Serum adiponektin seviyelerinin meme kanseri ile ilişkili olduğu bildirilmiştir. Fakat, adiponektin reseptörlerinin meme tümörü oluşumundaki rolü tam olarak ortaya konmamıştır. Gereç ve Yöntem: MMTV-TGF-α transgenik fareler 10 haftalıktan 74 haftalığa kadar beslendi. Meme tümörü geliştiren ve geliştirmeyen 74 haftalık transgenik farelerin karaciğer, meme (MFP) ve meme tümörü (MT) dokularında adiponektin, AdipoR1 ve AdipoR2 proteinlerinin ekspresyon seviyeleri western blot yöntemi kullanılarak belirlendi. Adiponektin seviyesi ELISA yöntemi ile ölçüldü. Bulgular: Adiponektin ve AdipoR1 protein ekspresyon seviyeleri MT geliştiren farelerde, MT geliştirmeyen farelere göre anlamlı olarak daha azdı. Fakat, MT-pozitif ve MT-negatif farelerin MT ve MFP dokularındaki AdipoR2 proteininin ekspresyon seviyeleri benzerdi. MT-pozitif ve MT-negatif farelerin karaciğer dokusundaki adiponectin, AdipoR1 ve AdipoR2 protein ekspresyon seviyeleri de benzerdi. Ek olarak, MTpozitif ve MT-negatif farelerin serum adiponectin seviyeleri benzerdi. Sonuç: Bu sonuçlar adiponektin ve reseptörlerinin analiz edilen dokuya spesifik bağımlı olarak düzenlendiğini işaret etmektedir. Ayrıca, AdipoR1 ve adiponectin MT gelişiminde önemli rol oynuyor olabilir

Kaynakça

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Kaynak Göster

Southern Clinics of Istanbul Eurasia
  • ISSN: 2587-0998
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2017

1.1b386

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