Strawberry is a delicious fruit that can be consumed fresh or/and can be used in the industry. One of the important factors that affect yield is the plant protection problems. Fungicides because of the metabolites they produce, especially their presence in foods, pose a threat to health and also cause economic problems. Botrytis cinerea causes grey mold disease in over 220 plant species. Samples were collected from five different localities from Aydın and Mersin province. Samples were incubated on um and then isolated from mixed cultures. Morphological and molecular identification of the samples were made. Transposon sites were amplified with PCR. In total 154 B.cinerea samples were isolated of which Transposa 20.1%, Boty 46.1%, Flipper 10.4% and Vacuma 23.4%, in Aydın population Transposa 19.5%, Boty 48.3%, Flipper 9.2% and Vacuma 23.0%, in Mersin Transposa 20.9%, Boty 43.3%, Flipper 11.9% and Vacuma 23.9% were found. In the fungicide resistant test, in spore tests, Cyprodinil was found most effective while fenhexamid was found less effective. In mycelium tests, fenhexamid was most effective at the lowest concentration applied while carbendazim was found less effective even at the highest concentration. Although short-term results are limited, in long-term transposon profiling can help with disease management.