3 Farklı Dentin Hassasiyeti Giderici Diş Macununun Sitotoksisitesinin Değerlendirilmesi

Amaç: Hassasiyet giderici diş macunları uygulama süreleri boyunca dişler ve oral dokularla sürekli temas halindedirler. Macunların içindeki ajanlar ideal olarak oral dokulara zarar vermemeli, dokuların iyileşmelerine yardımcı olmalıdırlar. Bu çalışmanın amacı, dentin hassasiyeti gidermek için sıklıkla kullanılan üç farklı diş macunun L929 fare fibroblast hücrelerinin canlılıkları üzerine etkilerini değerlendirmektir. Gereç ve Yöntemler: Test edilen macunların orijinal ekstraktı Dulbecco's Modified Eagle Medium (DMEM) kültür ortamı ile seyreltilerek üç farklı konsantrasyonu hazılandı. L929 fare fibroblastı hücreleri macunların %25, %12, %6’lık üç farklıkonsantrasyonuna maruz bırakıldı (n=12). Hücre canlılığı MTT testi ile değerlendirildi. Kontrol grubunun hücre canlılığı %100’e eşitlendi, veriler istatistiksel olarak one-way ANOVA ve post-hocTukey’s HSD testleriyle değerlendirildi. Bulgular: Test edilen macunların %25, %12, %6’lık üç farklı konsantrasyonun da kontrol grubuyla karşılaştırıldığında L929 hücreleri üzerine sitotoksik etkileri olduğu gözlenmiştir (p

Cytototoxic Evaluation of Three Different Dentin Desensitizing Toothpastes

Background: Dentin desensitizing toothpastes are in constant contact with the teeth and oral tissues during their application time. Ideally, the agents in the toothpastes should not damage the oral tissues and help the healing of them. The aim of the study is to evaluate the effects of three different common used dentin desensitizing toothpasteson the viability of L929 mouse fibroblast cells. Methods: Three different concentrations were prepared with the original extract of the tested toothpastes was diluted with Dulbecco's Modified Eagle Medium. L929 were exposed to three different concentrations of the 25%, 12%, 6% toothpastes (n=12). Cell viability was evaluated with XTT assay. The mean values of control groups were set to represent 100% viability. Data were statistically evaluated with one-way ANOVA and post-hocTukey's HSD tests. Results: It was observed that the three different concentrations of 25%, 12%, 6% of the tested toothpastes had cytotoxic effects on L929 cells compared to the control group (p

PDF

___

1. Bekes K, John MT, Schaller HG, Hirsch C. Oral health- related quality of life in patients seeking care for dentin hypersensitivity. J. Oral Rehabil 2009;36:45-51.
2. Canadian Advisory Board on Dentin Hypersensitivity. Consensus-based recommendations for the diagnosis and management of dentin hypersensitivity. Journal of the Canadian Dental Association 2003;69(4):221-226.
3. Li Y. Innovations for combating dentin hypersensitivity: Current state of the art. Compend Contin Educ Dent. 2012;33(special number 2):10-16.
4. Splieth CH, Tachou A. Epidemiology of dentin hypersensitivity. Clin Oral Investig2013;17(1):3-8.
5. Taha S. The prevalence of Dentine Hypersensitivity in Dentine Hypersensitivity: Advances in Diagnosis, Management, and Treatment. Springer International Publishing 2015:41-47.
6. Holland GR, Nahri MN, Addy M, Gangarosa L, Orchardson R. Guidelines for the design and conduct of clinical trials on dentine hypersensitivity. J Clin Periodontol 1997;24: 808-813.
7. Grippo JO. Abfractions: A new classification of hard tissue lesions of teeth. J Esthet Dent 1991;3:14-19.
8. Branström M. Dentin sensitivity and aspiration of odontoblasts. Journal of the American Dental Association 1963;66:366-370.
9. Addy M, West NX. The role of toothpaste in the aetiology and treatment of dentine hypersensitivity. Monogr Oral Sci 2013;23:75-87.
10.Jacobsen PL, Bruce G. Clinical dentin hypersensitivity: Understanding the causes and prescribing a treatment. J Contemp Dent Pract 2001;2(1):1-12.
11.Cummins D. Advances in the clinical management of dentin hypersensitivity: A review of recent evidence for the efficacy of dentifrices in providing instant and lasting relief. J Clin Dent 2011;22(4):100-107.
12.Pashley DH. Dynamics of the pulpo-dentinal complex. Crit Rev Oral Biol Med 1996;7(2):104-133.
13.Irwin CR, McCusker P. Prevalence of dentine hypersensitivity in a general dental population. J Ir Dent Assoc 1997;43(1):7-9.
14.Hines D, Xu S,Stranick M,Lavender S,Pilch S,ZhangYP, Sullivan R, Montesani L, LR Mateo, WilliamsM, et al. Effect of a stannous fluoride toothpaste on dentinal hypersensitivity: In vitro and clinical evaluation. J Am Dent Assoc 2019;150:47-59.
15.Ajcharanukul O, Kraivaphan P, Wanachantararak S, Vongsavan V, Matthews B. Effects of potassium ions on dentine sensitivity in man. Arch Oral Biol 2007;52(7):625-663.
16.Lippert F. An introduction to toothpaste – its purpose, history and ingredients. Monogr Oral Sci 2013;23:1-14. 17.Van Loveren C. Toothpastes. Monographs in Oral Science 2014.
18.Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, Jeng JH, et al. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 2005;7:745-753.
19.Huang FM, Chang YC. Cytotoxicity of dentine bonding agents on human pulp cells in vitro. Int Endod J2002;35(11):905-909.
19.Huang FM, Chang YC. Cytotoxicity of dentine bonding agents on human pulp cells in vitro. Int Endod J2002;35(11):905-909.
20.Herlofson BB, Barkvoll P. The effect of two toothpaste detergents on the frequency of recurrent aphthous ulcers. Acta Odontol Scand 1996;54:150-153.
21.De Groot A. Contact allergy to (ıngredients of) toothpastes. Dermatitis 2017;28:95-114.
22.Whitford GM. Acute and chronic fluoride toxicity. J Dent Res 1992;71:1249-54.
23.Schmalz G, Schuster U, Nuetzel K, Schweikl H. An in vitro pulp chamber with three-dimensional cell cultures. Journal of Endodontics 1999;25(1):24-9.
24.ISO B. 10993-5: Biological evaluation of medical devices. Tests for in vitro cytotoxicity 1999
25.Healy CM, Cruchley AT, Thornhill MH, Williams DM. The effect of sodium lauryl sulphate, triclosan and zinc on the permeability of normal oral mucosa. Oral Dis 2000;6:118–123.
26.Veys RJ, Baert JH, De Boever JA.Histological changes in the hamster cheek pouch epithelium induced by topical application of sodium lauryl sulphate. Int J Exp Pathol 1994;75(3):203-9.
27.Herlofson BB, Barkvoll P. Desquamative effect of sodium lauryl sulfate on oral mucosa: A preliminary study. Acta Odontol Scand 1993;51:39-43.
28.Shim YJ, Choi JH, Ahn HJ, Kwon JS. Effect of sodium lauryl sulfate on recurrent aphthous stomatitis: A randomized controlled clinical trial. Oral Dis 2012;18:655-660.
29.Moore C, Addy M, Moran J. Toothpaste detergents: A potential source of oral soft tissue damage? Int J Dent Hyg 2008;6:193-198.
30.Rantanen I, Tenovuo J, Pienihakkinen K, Soderling E. Effects of a betaine-containing toothpaste on subjective symptoms of dry mouth: A randomized clinical trial. J Contemp Dent Pract 2003;4:11-23.
31.BenassiL, Bertazzoni G, MagnoniC, Rinaldi M, Fontanesi C, Seidenari S. Decrease in toxic potential of mixed tensides maintained below the critical micelle concentration: An in vitro study. Skin Pharmacol Appl Skin Physiol 2003;16:156-164.
32.Cvikl B, Lussi A, Gruber R. The in vitro impact of toothpaste extracts on cell viability. Eur J Oral Sci 2015;123:179-185.
33.Cummins, D. Recent advances in dentin hypersensitivity: clinically proven treatments for instant and lasting sensitivity relief. Am J Dent 2010;23:3-13.
34.Petrou I, Heu R, Stranick M, Lavender S, Zaidel L, Cummins D. A breakthrough therapy for dentin hypersensitivity: How dental products containing 8% arginine and calcium carbonate work to deliver effective relief of sensitive teeth. J Clin Dent 2009;20:23-31.
35.GB Eyuboğlu, C Yesilyurt, M Ertürk. Evaluation of cytotoxicity of dentin desensitizing products. Operative Dentistry 2015;40-5:503-514.
36.Camargo SEA, Milhan NVM, Saraiva FO, Oliveira JR, Oliveira LD, Camargo CHR. Are desensitizing toothpastes equally biocompatible and effective against microorganisms? Braz Dent J 2017;28(5):604- 611.
37.Sadat-Shojai M, Khorasani MT, Dinpanah-Khoshdargi E, Jamshidi A. Synthesis methods for nanosized hydroxyapatite withdiverse structures. Acta Biomaterialia 2013;9:7591-7621.
38.Vano M, Derchi G, Barone A, Pinna R, Usai P, Covani U. Reducing dentine hypersensitivity with nano- hydroxyapatite toothpaste: A double-blind randomized controlled trial. Clinical Oral Investigations 2018;22:313-320.
39.Jena A, Kala S, Shashirekha G. Comparing the effectiveness of four desensitizing toothpastes on dentinal tubule occlusion: A scanning electron microscope analysis. Journal of Conservative Dentistry 2017;20:269-272.
40.Low SB, Allen EP, Kontogiorgos ED. Reduction in dental hypersensitivity with nano-hydroxyapatite, potassium nitrate,sodium monoflurophosphate and antioxidants. The Open Dentistry Journal2015:92-97.
41.Bossu M,Saccucci M, Salucci A, Di Giorgio G, Bruni E, Uccelletti D, Sarto MS, Familiari G, Relucenti M, PolimeniA,et al. Enamel remineralization and repair results of biomimetic hydroxyapatite toothpaste on deciduous teeth: Aneffectiveoption to fluoride toothpaste. Journal of Nanobiotechnology 2019;17:17.
42.Epple M. Review of potential health risks associated with nanoscopic calcium phosphate. Acta Biomaterialia 2018;77:1-14.
43.Coelho CC, Grenho L, Gomes PS, Quadros PA, Fernandes MH. Nano-hydroxyapatite in oral care cosmetics: Characterization and cytotoxicity assessment Scientific Reports 2019;9:11050.
44.Shiau HJ. Dentin hypersensitivity. J Evid Based Dent Pract 2012;12:220-228.
45.Addy M, Mostafa P. Dentine hypersensi¬tivity: Effects produced by the uptake in vitro of metal ions, fluoride and form¬aldehyde onto dentine. J Oral Rehab 1988; 15:575-585.
46.Lee JH, Jung JY, Jeong YJ. Involvement of both mitochondrial-and death receptor-dependent apoptotic pathways regulated by Bcl-2 family in sodium fluoride-induced apoptosis of the human gingival fibroblasts. Toxicology 2008;243:340-347.
47.Qu WJ, Zhong DB, Wu PF. Sodium fluoride modulates caprine osteoblast proliferation and differentiation. J Bone Miner Metab 2008;26:328-334.
48.Arakawa Y, Bhawal UK, Ikoma T. Low concentration flüoride stimulates cell motility of epithelial cells in vitro. Biomed Res 2009;30:271-277.
49.Kitchin PC, Graham WC. Sodiumalkylsulfate as a detergent in toothpaste (1937) J AmDentAssoc 24: 736-755
50.Schmalz G, Arenholt-Bindslev D. CHAPTER, In: Biocompatibility of DentalMaterials (Vol 1). Berlin: Springer; 2009, p. x