Umut YİĞİT, Fatih KARAASLAN, Ahu DİKİLİTAŞ, Esra Özge AYDIN

PERİODONTİTİS DERECESİ İLE DİŞETİ OLUĞU SIVISI TNF-α VE MIP-1α SEVİYELERİ ARASINDAKİ İLİŞKİNİN DEĞERLENDİRİLMESİ: ÖN ÇALIŞMA

Amaç Periodontitisin patogenezindeki sitokin ve kemokinlerin rolü,periodontitisin başlaması ve ilerlemesinde önemli fonksiyonlara sahip olan ile Tümör Nekroz Faktör alfa (TNF-α) ve Makrofaj İnflamatuar Protein 1α ile (MIP 1α) gösterilmiştir. Bununla birlikte, farklı periodontitis derecelerinin sitokin ve kemokin profilleri hala belirsizdir ve periodontitisin ilerleme hızı ile ilişkili biyobelirteçler hakkında henüz kesin bilgiler bildirilmemiştir. Bu nedenle, bu çalışmanın amacı Derece A, B ve C'deki dişeti oluğu sıvısındaki (DOS) MIP-1α ve TNF-α'nın düzeylerini tahmin etmek ve periodontitis derecesini belirlemede güvenilir biyobelirteçler olarak rollerini değerlendirmektir. Gereç ve Yöntem Bireyler periodontitis derecelerine göre Evre IV periodontitis tanısı alan ve Derece A (Derece A, n = 21), Evre IV periodontitis tanısı alan ve Derece B (Derece B, n =21) ve Evre IV periodontitis tanısı alan Derece C bireyler (Derece C, n = 21) olmak üzere üç gruba ayrıldı. Bulgular Ortalama TNF-α seviyeleri açısından gruplar arasında anlamlı bir fark olmamasına rağmen, Derece C’deki ortalama MIP-1α seviyesi Derece B ve Derece A'dan anlamlı derecede yüksekti. Derece B'deki ortalama MIP-1α seviyesi Derece A'dan önemli ölçüde daha yüksek (p < 0.05, Kruskal–Wallis testi) bulundu. Sonuç: MIP-1α, periodontitis derecesi için tanımlayıcı bir biyobelirteç olarak klinik kullanıma sahip olabilir.

Anahtar Kelimeler:

kemokin, sitokin, dişeti oluğu sıvısı , , periodontitis

THE ASSOCIATION BETWEEN GRADE OF PERIODONTITIS AND GCF LEVELS OF TNF-α AND MIP-1α: A PRELIMINARY STUDY

Objective The role of cytokines and chemokines in the pathogenesis of periodontitis indicates that tumor necrosis factor alpha (TNF-α) and macrophage inflammatory protein 1α (MIP1α) have crucial functions in the initiation and progression of periodontitis. However, the cytokine and chemokine profiles of different grades of periodontitis are still unclear, and no conclusive information has yet been reported on biomarkers associated with the progression rate of periodontitis. Thus, the aim of the present study was to estimate the gingival crevicular fluid (GCF) levels of MIP-1α and TNF-α in Grades A, B, and C and to evaluate their role as reliable biomarkers in determining the grade of periodontitis. Materials and Methods Individuals were divided into three groups according to their grade of periodontitis: individuals diagnosed as Stage IV periodontitis with Grade A (Grade A, n = 21), individuals diagnosed as Stage IV periodontitis with Grade B (Grade B, n = 21), and individuals diagnosed as Stage IV periodontitis with Grade C (Grade C, n = 21). Results Although there were no significant differences between groups in terms of mean TNF-α levels, the mean MIP- 1α level of Grade C was significantly higher than that of Grade B and Grade A. The mean MIP-1α level of Grade B was significantly higher than that of Grade A (p < 0.05, Kruskal–Wallis test). Conclusion MIP-1α could have clinical utility as a screening biomarker for the grade of periodontitis.

Keywords:

Chemokines, cytokines, gingival crevicular fluid, periodontitis,

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