Experimental 70% Hepatectomy Model: Apoptotic Index, Proliferative Index and Mitotic Index

Liver regeneration is known to begin after experimentally induced liver resection. At first 24- 48 hours, DNA synthesis reaches the maximum level after resection and significantly regeneration known to occur on the first 10 days. In this study, we were examined the regeneration of liver on the 1st and 14th days after the resection. In experiment 21 Wistar albino male rats were used. The rats were randomly divided into three groups. The first group was the control, the second group was sacrificed one day after hepatectomy, and the third group was sacrificed 14 days after hepatectomy. All experimentally groups realized midline incision with laparotomy for resection of liver. Pedicles of the left lateral and median lobes of the liver were applied 70% hepatectomy by 4/0 silk binding. At the end of 1st and 14th days, liver tissue removed for light misroscobic analysis. No histopathological findings were found in the control group. The tissue of all experimentally groups were showed some histopatological changes such as sinuzoidal dilatation, vacuolization in the hepatocytes. This histopatological findings were seem to increase at grup II more than group III and group I with routin H&E staining. Mitotic index, apoptotic index and proliferation index of values at group II was reached the maximum level. Group III dramatically reduced the value of these index and were seem to reach near to values of the control group. Relative liver weight that determined each of experimentally groups were statistically significant differences compared to the control group. As a result, in this study, liver regeneration was shown in liver transplants without any agent that would contribute to the clinic.

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