METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was evaluated for early diagnosis of OSCC by non-invasive methods. Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by statistical analysis. Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%. We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree and expression in OSCC. Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other mechanisms need to be explored to understand the differences in expression levels.
METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was evaluated for early diagnosis of OSCC by non-invasive methods. Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by statistical analysis. Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%. We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree and expression in OSCC. Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other mechanisms need to be explored to understand the differences in expression levels.
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- Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
