Ratlarda deneysel spinal kord yaralanmasında Hesperidin’in nöroprotektif etkisi

Amaç: Bu çalışmada, ratlarda deneysel omurilik yaralanmasında farklı dozlarda hesperidinin (HSP) oksidatif hasara karşı koruyucu etkisini histopatolojik ve biyokimyasal değerlendirmeler kullanarak araştırmayı amaçladık.Gereç ve yöntem: Çalışma 4 grup ve her grupta 10 adet Wistar albino cinsi rat olacak şekilde planlandı. Grup 1 (kontrol grubu): laminektomi yapıldı. Laminektomi sonrası üç gruba (G2, G3, G4) travma uygulandı. Grup 2 (patoloji grubu): laminektomi yapıldı ve ekstradural klip uygulandı. Grup 3 (düşük doz HSP): laminektomi yapılıp, ekstradural klip uygulandı ve tek doz düşük doz HSP intraperitoneal olarak verildi (50 mg/kg). Grup 4 (yüksek doz HSP): laminektomi yapılıp, ekstradural klip uygulandı ve tek doz yüksek doz HSP intraperitoneal olarak verildi (100 mg/kg). Nöroprotektif etkiyi değerlendirmek için alınan doku örnekleri biyokimyasal ve histopatolojik olarak incelendi.Bulgular: Travma gruplarındaki inflamatuar bulgular ve nöron sayısının morfometrik sonuçları, Grup 4‘te diğer gruplara kıyasla istatiksel olarak anlamlı şekilde daha iyiydi. Klinik motor muayene ve eğik düzlem test sonuçları açısından gruplar arasında fark yoktu. Her iki tedavi grubunda (Grup3, Grup4), lipid peroksidasyonunun son ürünü olan malondialdehit anlamlı bir farklılık gözlemlenmezken (p>0,05); total antioksidan plazma seviyelerinde kontrol ve patoloji gruplarına kıyasla istatistiksel olarak anlamlı bir farklılık gözlemlendi (p:0,001). Sonuç: Sonuçlarımız, yüksek doz HSP’ nin spinal kord hasarında nöronların sayısındaki azalmaya olumlu etkisi ve hafif dejenerasyon bulguları ile neuroprotektif etki sağlayabileceğini düşündürmektedir.

Anahtar Kelimeler:

Hesperidin, spinal kord yaralanma, neuroprotektif etki

Neuroprotective effect of Hesperidin on experimental spinal cord injury in rats

Purpose: In this study, we aimed to investigate the protective effect of different doses of Hesperidin (HSP) against oxidative damage in experimental spinal cord injury in rats using histopathological and biochemical evaluations.Material and Methods: The study was planned as four groups and 10 Wistar albino rats in each group. Group 1 (control group): laminectomy was performed. Trauma was applied to three groups (G2, G3, G4) after laminectomy. Group 2 (pathology group): laminectomy was performed, and an extradural clip was applied. Group 3 (low dose HSP): laminectomy was performed, an extradural clip was applied, and a single low dose HSP was administered intraperitoneally (50 mg/kg). Group 4 (high-dose HSP): laminectomy was performed, an extradural clip was applied, and a single high-dose HSP was administered intraperitoneally (100 mg/kg). Tissue samples taken to evaluate the neuroprotective effect were examined biochemical and histopathological.Results: Morphometric results of inflammatory findings and neuron number in the trauma groups were significantly better in Group 4 than in the other groups. There was no difference between the groups regarding clinical motor examination and inclined plane test results. Malondialdehyde, the end product of lipid peroxidation, did not differ significantly in both treatment groups (Group3, Group4) (p>0.05); A statistically significant difference was observed in total antioxidant plasma levels compared to the control and pathology groups (p:0.001).Conclusion: Our results suggest that high-dose HSP may provide a neuroprotective effect with its positive impact on decreasing the number of neurons in spinal cord damage and mild degeneration findings.

Keywords:

Hesperidin, spinal cord injury, neuroprotective effect,

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