Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?

Purpose: To investigate of histopathologic and biochemical effects of caffeine citrate in newborn rats with hypoxia/reoxygenation (H/R)-induced intestinal injury. Materials and methods: One-day-old, 32 Wistar albino newborn rats (n=8) were randomly divided into four groups: control group (group1, n=8), caffeine group (group2, caffeine citrate administered subcutaneously, n=8), H/R group (group3, exposed to H/R, n=8), and caffeine + H/R group (group4, caffeine citrate administered and exposed to H/R, n=8). Caffeine citrate was initiated at a loading dose of 20 mg/kg, followed by a maintenance dose of 5 mg/kg/day, subcutaneously. On day 4th, all animals except for groups 1 and 2 were exposed to H/R and sacrificed 6 hours after H/R procedure. Histopathological injury scores (HISs), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and oxidative stress index (OSI: total oxidant status “TOS”/total antioxidant status “TAS”) levels were measured in intestinal samples. Results: As histopathological, the most severe damage was observed in H/R-induced groups (p

Kafein sıçan yavrularında bağırsak hasarı gelişiminde predispozan bir risk faktörü mü?

Amaç: Hipoksi/reoksijenizasyon (H/R) ile intestinal hasarlanma oluşturulan sıçan yavrularında kafein sitratın histopatolojik ve biyokimyasal etkilerini araştırmak. Gereç ve yöntem: Bir günlük 32 Wistar albino sıçan yavrusu rastgele dört gruba ayrıldı: kontrol grubu (grup1, n=8), kafein grubu (grup2, subkutan kafein sitrat uygulanan, n=8), H/R grubu (grup3, H/R uygulanan, n=8) ve kafein+H/R grubu (grup4, kafein sitrat verilen ve H/R uygulanan, n=8). Kafein sitrat, 20 mg/kg'lık bir yükleme dozunda başlatıldı, ardından subkutan 5 mg/kg/günlük idame dozu takip edildi. Dördüncü günde, grup 1 ve 2 dışındaki tüm hayvanlar H/R'ye maruz bırakıldı ve H/R prosedüründen 6 saat sonra öldürüldü. Histopatolojik hasarlanma skorları (HIS), interlökin-6 (IL-6), tümör nekroz faktörü-alfa (TNF-α) ve oksidatif stres indeksi (OSI: toplam oksidan durum “TOS”/toplam antioksidan durum “TAS”) seviyeleri bağırsak örneklerinde değerlendirildi. Bulgular: Histopatolojik olarak en ciddi hasar H/R uygulanan gruplarda görüldü (p

Kaynakça

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Kaynak Göster

  • ISSN: 1309-9833
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2008

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