Biyoteknolojik uygulamalar için limon suyundan karbon noktalarının mikrodalga bazlı sentezi

Bu çalışmada, doğal bir sitrik asit ve azot/kükürt kaynağı olan limon suyundan karbon noktalarının (CD'ler) sentezi için basit bir mikrodalga yöntemi kullanılmıştır. Bilim yazınında, limon suyundan CD sentezi için hidrotermal temelli yöntemler kullanılmıştır. Daha basit ve hızlı olması açısından bu çalışmada mikrodalga yönteminden karbon noktalarının sentezi araştırılmıştır. Hidrotermal bazlı yöntemlerle karşılaştırıldığında, CD'lerin kuantum verimi oldukça düşük bulumuştur (%0.74). Bununla birlikte, sentezlenen CD'ler, biyoteknolojide kullanılan sığır serum albümin (BSA) proteinlerinin floresans ışımasını mükemmel şekilde sönümlediği göstermiştir. Ayrıca, S. epidermidis, C. albicans, S. aureus, P. aeruginosa, E. coli, E. feacalis, K. pneumonia, A. baumanii gibi klinik olarak önemli hastalık etkenleri üzerinde çok yüksek mikrop-kıran (antimikrobiyal) etkilere sahip olduğu bulunmuştur.

Anahtar Kelimeler:

Karbon noktalarının sentezi (karbon dot sentezi), mikrodalga yöntemi, Bovin Serum Albumin (BSA) sönümleme, saptama, antimikrobiyal aktivite.

Microwave-Based Synthesis of Carbon Dots From Lemon Juice For Biotechnological Applications

In this study, a simple microwave method was used for the synthesis of carbon dots (CDs) from lemon juice as a natural source of citric acid and nitrogen/sulfur. In the synthesis of CDs from lemon juice, hydrothermal-based methods were used in the literature. In terms of being simpler and faster, the synthesis of carbon dots from the microwave method was investigated in this study. Compared to the hydrothermal-based methods, the quantum yield of CDs obtained by this method was found to be quite low (0.74%). However, the synthesized CDs showed quenching features over bovine serum albumin (BSA) proteins, which can be used in biotechnology. Moreover, it has been found to have antimicrobial effects on clinically important pathogens such as S. epidermidis, C. albicans, S. aureus, P. aeruginosa, E. coli, E. feacalis, K. pneumonia, and A. baumanii.

Keywords:

Carbon dots (CDs), Micro-wave method, Micro-wave method , Bovine Serum Albumin (BSA, Bovine Serum Albumin (BSA), quenching/detection, Antimicrobial activity.,

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