Mustafa EFENDİOĞLU, Ezgi AKAR, Emine Emel KOÇMAN, Recep BAŞARAN, Gülbu IŞITMANGİL

Lomber Spinal Stenozun HLA İlişkisi Üzerine Ön Araştırma

Amaç: Biz bu çalışmada lumbar spinal stenosis (LSS) ile genetik HLA sistemi arasındaki ilişkiyi tespit etmeyi amaçladık.

Anahtar Kelimeler:

Human lökosit antijen, lomber spinal stenosis, sınıf I/II genler

A Preliminary Research on HLA-Association of Lumbar Spinal Stenosis

Objective: We aim to identify the relationship lumbar spinal stenosis (LSS) and genetic HLA system.Materials and Methods: The present study investigates 15 cases out of 41 (7 male, 8 female; average age: 60.27) who were studied for HLA class I ABC genes, as well as totally 41 cases (19 male; 22 female; average age: 60.46) who were studied for HLA class II DR.DQB1 genes among the cases with LSS. DNA extraction, the DNA was amplified with primers specific to HLA-A, B, C, DQ and DR, and studied using the SSP-PCR method. Allele identification was made using the official HLA Nomenclature Committee software.Results: HLA A2 allele in LSS group (%36.7) is statistically significantly higher than control group (%17.2) (p:0.039; p<0.05). The frequency of the B44 allele was statistically significantly higher in the LSS group (20%) than in the control group (5.1%) (p:0.010; p<0.05). Likewise, the frequency of the C6 allele was statistically significantly higher in the LSS group (26.7%) than in the control group (8.6%) (p:0.013; p<0.05). Conclusion: This preliminary study has suggested that LSS is associated with HLA class I genes. HLA A2, HLA B44, and HLA C6 alleles have been determined as predisposing factors for LSS.

Keywords:

Class I/II genes, human leukocyte antigen, lumbar spinal stenosis,

PDF

___

Karaeminoğulları O, Aydınlı U. Dejeneratif Lomber Spinal Stenoz. TOTBİD. 2004;3(4):105-113.
Akçam FZ. HLA System: Review. Turkiye Klinikleri J Med Sci. 2005;25(6):829-34.
Mosaad YM. Clinical Role of Human Leukocyte Antigen in Health and Disease. Scandind J Immunol. 2015;82(4):283–306.
Bingöl Ü. Etiopathogenesis in Ankylosing Spondylitis. Türkiye Klinikleri, J Immunol Rheumatol. 2011;4(1):7-15.
Alpsoy E. Behçet Hastalığı: Etyopatogenezde Güncel Bilgiler. Turk J Dermatol. 2013;7:41-45.
Noble JA, Valdes AM. Genetics of the HLA Region in the Prediction of Type 1 Diabetes. Curr Diab Rep. 2011;11(6):533–542.
Jiang H, Yang Q, Jiang J, Zhan X, Xiao Z. Association between COL11A1 (rs1337185) and ADAMTS5 (rs162509) gene polymorphisms and lumbar spine pathologies in Chinese Han population: an observational study. BMJ Open. 2017;7(5):1-8.
Hyun SJ, Park BG, Rhim SC, Jang JW, Jeon SR, Roh SW. Progression of lumbar spinal stenosis is influenced by polymorphism of thrombospondin 2 gene in the Korean population. Eur Spine J. 2014;23(1):57-63.
Hallioglu O, Mesci L, Ozer S. DRB1, DQA1, DQB1 genes in Turkish children with rheumatic fever. Clin Exp Rheumatol. 2005;23(1):117-120.
Zhang YG, Zhang F, Sun Z. A controlled case study of the relationship between environmental risk factors and apoptotic gene polymorphism and lumbar disc herniation. Am J Pathol. 2013;182:56–63.
Mayer JE, Iatridis JC, Chan D. Genetic polymorphisms associated with intervertebral disc degeneration. Spine J. 2013;13:299–317.
Atasoy M, Pirim I, Bayrak ÖF, et al. Association of HLA class I and class II alleles with psoriasis vulgaris in Turkish population. Inﬂuence of type I and II psoriasis. Saudi Med J. 2006;27(3): 373-376.
Doganay L, Katrinli S, Colak Y, et al. HLA DQB1 alleles are related with nonalcoholic fatty liver disease. Mol Biol Rep. Article in Molecular Biology Reports. 2014;41(12):7937-7943.
Tunca M, Musabak U, Sagkan RI, Koc E, Akar A. Association of human leukocyte antigen class II alleles with pemphigus vulgaris in a Turkish population. J Dermatol. 2010;37(3):246-250.
Zhang Y, Chen J, Zhong ZM, Yang D, Zhu Q. Is platelet-derived growth factor-BB expression proportional to fibrosis in the hypertrophied lumber ligamentum flavum? Spine (Phila Pa 1976). 2010;35(25):1479-1486.
Jirathanathornnukul N, Limthongkul W, Yingsakmongkol W, Singhatanadgige W, Parkpian V, Honsawek S. Increased expression of vascular endothelial growth factor is associated with hypertrophic ligamentum flavum in lumbar spinal canal stenosis. J Investig Med. 2016;64(4):882-887.