İnsan Pankreas Kanserinde Borik Asit Uygulaması ile Bazı IncRNA’ların ve İnvazyonun Baskılanması

Amaç: Borik asidin bazı lncRNA'lar ve invazyon üzerine etkisinin PANC-1 ve MIA PaCa-2 pankreas kanseri hücrelerinde araştırılması amaçlanmıştır. Materyal ve Metot: Borik asitin hücre canlılığı ve invazyon üzerine etkileri sırası ile XTT testi ve invazyon kuyucukları kullanılarak araştırılmıştır. TCGA verileri kullanılarak pankreas kanserinde lncRNA H19 ve UCA1 ifadeleri değerlendirilmiştir. Bu lncRNA’ların ve invazyon genlerinin ifadeleri üzerine etkileri qRT-PZR ile belirlenmiştir. Bulgular: PANC-1 hücrelerinde borik asitin IC50 dozu 14.25 mM, MIA PaCa-2 de ise 15.71 mM olarak hesaplanmıştır. TCGA verileri H19 ve UCA1 ifadelerinin pankreas kanserinde yüksek olduğunu göstermiştir. Borik asit muamelesi ile H19 ve UCA1 lncRNA seviyeleri azalmıştır. Ayrıca borik asit iki hücre hattında CDH1 ve TIMP1 ifadesini arttırmıştır. CDH2 ifadesini baskılamıştır. Borik asit PANC-1 hücrelerinde TIMP2, MIA PaCa-2 hücrelerinde TIMP3 ifadesini arttırmıştır. İnvazyon testinde borik asit her iki hücrede invazyonu anlamlı derecede baskılanmıştır. Sonuç: Borik asit pankreas kanserinde ifadesinin yüksek olduğu görülen H19 ve UCA1 ifadelerini baskılamıştır. Ayrıca invazyonda önemli olan genlerin ifadelerini değiştirerek anti-invaziv etki göstermiştir.

Anahtar Kelimeler:

Borik asit, invazyon, lncRNA-H19, lncRNA-UCA1, pankreas kanseri

Suppression of some IncRNAs and Invasion by Boric Acid Treatment in Human Pancreatic Cancer

Objective: It was aimed to investigate the effects of boric acid on some lncRNAs and invasion in PANC-1 and MIA PaCa-2 pancreatic cancer cells. Materials and Methods: The effects of boric acid on cell viability and invasion were investigated using XTT test and invasion chambers, respectively. lncRNA H19 and UCA1 expressions were evaluated in pancreatic cancer using TCGA data. Its effects on expressions of these lncRNAs and invasion genes were determined by qRT-PCR analysis. Results: The IC50 doses of boric acid were calculated as 14.25 mM in PANC-1 cells and 15.71 mM in MIA PaCa-2. TCGA data showed that H19 and UCA1 expressions were elevated in pancreatic cancer. H19 and UCA1 lncRNA levels were decreased with boric acid treatment. In addition, boric acid increased CDH1 and TIMP1 in both cell lines. However, it suppressed CDH2 expression. Boric acid increased TIMP2 in PANC-1 cells and TIMP3 expression in MIA PaCa-2 cells. In the invasion test, boric acid significantly suppressed invasion in both cells. Conclusions: Boric acid suppressed H19 and UCA1 expressions, which were found to be high in pancreatic cancer. In addition, it showed an anti-invasive effect by changing the expressions of genes that are important in invasion.

Keywords:

Boric acid, invasion, lncRNA-H19, lncRNA-UCA1, pancreatic cancer,

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