Askorbik Asit ve Sodyum Selenitin Renal İskemi-Reperfüzyon Hasarında İnflamatuar Cevap ve Apoptoz Üzerine Etkileri

Amaç: Bu çalışmanın amacı, renal iskemi ve reperfüzyon (IR) hasarında askorbik asit (AA) ve sodyum selenit (SS) uygulamasının anti-inflamatuar ve anti-apoptotik etkilerini araştırmaktır. Materyal ve Metot: Yirmi sekiz Wistar-albino sıçan dört gruba ayrıldı. Grup I; Kontrol grubu, Grup II; IR grubu, Grup III; IR+0,25mg/kg SS ve 100mg/kg AA ve Grup IV; IR+0,50mg/kg SS ve 200mg/kg AA. Kontrol grubuna sadece cerrahi stres oluşturuldu. IR grubunda, sağ nefrektomi yapıldıktan sonra sol böbrekte 60 dk iskemi ve 24 saat reperfüzyon sağlandı. Grup III ve Grup IV’e IR grubundaki işleme ek olarak IR işleminden 5 gün önce belirlenen dozlarda SS ve AA uygulandı. Bulgular: Grup I, Grup III ve Grup IV ile karşılaştırıldığında Grup II‘de glomerulus ve renal tübüllerin morfolojik yapılarında şiddetli hasar oluştuğu görüldü (p<0,05). Grup I, Grup III ve Grup IV ile karşılaştırıldığında Grup II'de TNF-α, IL-6, Kaspaz-3 ve Bcl-2 ekspresyonu ve pozitif hücre yüzdesi istatistiksel olarak arttı (p<0,001). Sonuç: Bu çalışma, askorbik asit ve sodyum selenit'in profilaktik olarak uygulanmasının akut böbrek hasarına karşı koruyucu etkiler gösterdiğini belirledi.

Anahtar Kelimeler:

Askorbik asit, inflamasyon, renal iskemi-reperfüzyon, sodyum selenit

Effects of Ascorbic Acid and Sodium Selenite on Inflammatory Response and Apoptosis in Renal Ischemia-Reperfusion Injury

Objective: The aim of this study is to investigate the anti-inflammatory and anti-apoptotic effects of ascorbic acid (AA) and sodium selenite (SS) administration in renal ischemia and reperfusion (IR) injury. Materials and Methods: Twenty-eight Wistar-albino rats were divided into four groups. Group I; Control group, Group II; IR group, Group III; IR+0.25mg/kg SS and 100mg/kg AA and Group IV; IR+0.50mg/kg SS and 200mg/kg AA. In the control group, only surgical stress was created. In the IR group, 60 minutes of ischemia and 24 hours of reperfusion were applied in the left kidney after right nephrectomy. SS and AA were administered to Groups III and IV at doses determined 5 days before the IR procedure. Results: In comparison of Group II with Group I, Group III and Group IV; severe damage was observed in the morphological structures of the glomerulus and renal tubules in Group II (p<0.05). Compared to Group I, Group III and Group IV the expression of TNF-α, IL-6, Caspase-3, and Bcl-2 and the percentage of positive cells increased statistically in Group II (p<0.001). Conclusion: This study determined that prophylactic administration of ascorbic acid and sodium selenite showed protective effects against acute kidney injury.

Keywords:

Ascorbic acid, inflammation, renal ischemia-reperfusion, sodium selenite,

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