Genipin ile çapraz bağlı nanokapsüllerin sentezi ve karakterizasyonu

Yapılan çalışmada doğal bir çapraz bağlayıcı olan genipin kullanılarak sığla esansiyel yağının jelatin ve Arap zamkı ile nanoenkapsülasyonu amaçlanmıştır. Sentez sürecinde kompleks koaservasyon yöntemi kullanılarak ilk olarak sığla yağı jelatin ve Arap zamkı duvar materyalleri ile kaplanmış ve daha sonrasında farklı miktarlarda (150, 250, 350 ve 450 mg) genipin kullanılarak çapraz bağlanması sağlanmıştır. Elde edilen nanokapsüllerin toplam yağ, yüzey yağı ve enkapsülasyon etkinlik değerleri analiz edilmiştir. Genipin miktarının artışı ile nanokapsüllerin yüzey yağ miktarlarında azalış gözlenirken, toplam yağ ve enkapsülasyon etkinlik değerlerinde artış gözlemlenmiştir. 450 mg genipin kullanımı ile %91.78 enkapsülasyon etkinlik değerine sahip nanokapsüller elde edilmiş olup, bu nanokapsüllerin yapısal, morfolojik ve termal özelliklerinin incelenmesi için sırasıyla FTIR, FESEM, TGA ve DSC analizleri gerçekleştirilmiştir. Elde edilen sonuçlar sığla yağının jelatin ve Arap zamkı ile nano boyutta başarılı bir şekilde kaplandığını göstermektedir.

Anahtar Kelimeler:

Nanoenkapsülasyon, Çapraz bağlanma, Genipin, Kompleks koaservasyon, Sığla yağı

Synthesis and characterization of genipin-crosslinked nanocapsules

In this study, it was aimed to nanoencapsulate sweetgum essential oil with gelatin and gum arabic using genipin, a natural cross-linker. In the synthesis process, firstly, sweetgum oil was coated with gelatin and gum Arabic wall materials using the complex coacervation method, and then cross-linked using different amounts of genipin (150, 250, 350 and 450 mg). Total oil, surface oil, and encapsulation efficiency of the obtained nanocapsules were analyzed. With the increase in the amount of genipin, a decrease was observed in the surface oil content of the nanocapsules, while an increase was observed in the total oil and encapsulation efficiency values. Nanocapsules with an encapsulation efficiency value of 91.78% were obtained by using 450 mg genipin, and FTIR, FESEM, TGA, and DSC analyzes were performed to examine the structural, morphological and thermal properties of these nanocapsules, respectively. The results show that sweetgum oil is successfully coated with gelatin and gum Arabic at nanoscale.

Keywords:

Nanoencapsulation, Cross-linking, Genipin, Complex coacervation, Sweetgum oil,

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